Life Science / Plant molecular biology and physiology

Life Science / Applied microbiology

Life Science / Applied biochemistry

Life Science / Genome biology

Energy transfer and primary charge separation upon selective femtosecond excitation at 810 nm in the reaction center complex from Heliobacterium modesticaldum Reviewed

Risa Kojima, Hayata Yamamoto, Chihiro Azai, Chiasa Uragami, Hideki Hashimoto, Daisuke Kosumi, Hirozo Oh-oka

Journal of Photochemistry and Photobiology A: Chemistry   401   112758   2020.10

Mutation of alanine-422 in KaiC leads to a low amplitude of rhythm in the reconstituted cyanobacterial circadian clock.

Kazuki Nagata, Katsuaki Oyama, Atsushi Ota, Chihiro Azai, Kazuki Terauchi

The Journal of general and applied microbiology   66 ( 2 )   140 - 146   2020.6

Synechocystis KaiC3 Displays Temperature- and KaiB-Dependent ATPase Activity and Is Important for Growth in Darkness Reviewed International coauthorship

Anika Wiegard, Christin Köbler, Katsuaki Oyama, Anja K. Dörrich, Chihiro Azai, Kazuki Terauchi, Annegret Wilde, Ilka M. Axmann

Journal of Bacteriology   202 ( 4 )   e00478-19   2020.1

Heliobacterium modesticaldum光合成反応中心への選択励起における励起エネルギー移動および電子移動反応 Invited

小島理沙, 山元颯太, 浅井智広, 小澄大輔, 大岡宏造

2019.12

X-ray structure of the type-I reaction center from Heliobacterium modesticaldum at 3.2 A resolution Invited

NAKANIWA Tetsuko, MUTOH Risa, FUSHIMI Kokoro, YASUDA Aya, MIZOGUCHI Tadashi, TAMIAKI Hitoshi, AZAI Chihiro, TANAKA Hideaki, ITOH Shigeru, OH-OKA Hirozo, KURISU Genji

生物物理(Web)   58 ( Supplement 1-2 )   2018.9

Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria Reviewed

Takahiro Teramoto, Chihiro Azai, Kazuki Terauchi, Masashi Yoshimura, Toshiaki Ohta

Plant Physiology   177 ( 1 )   52 - 61   2018.3

Light-Induced Electron Spin-Polarized (ESP) EPR Signal of the P800⁺ Menaquinone^– Radical Pair State in Oriented Membranes of Heliobacterium modesticaldum: Role/Location of Menaquinone in the Homodimeric Type I Reaction Center Reviewed

Toru Kondo, Masahiro Matsuoka, Chihiro Azai, Masami Kobayashi, Shigeru Itoh, Hirozo Oh-oka

The Journal of Physical Chemistry B   122 ( 9 )   2536 - 2543   2018.2

Rapid C8-vinyl reduction of divinyl-chlorophyllide a by BciA from Rhodobacter capsulatus Reviewed

Chihiro Azai, Manami Kobayashi, Tadashi Mizoguchi, Hitoshi Tamiaki, Kazuki Terauchi, Yusuke Tsukatani

Journal of Photochemistry and Photobiology A: Chemistry   353   661 - 666   2018.2

Phosphorylation at Thr432 induces structural destabilization of the CII ring in the circadian oscillator KaiC Reviewed

Katsuaki Oyama, Chihiro Azai, Jun Matsuyama, Kazuki Terauchi

FEBS Letters   592 ( 1 )   36 - 45   2017.12

Determination of carbon-to-nitrogen ratio in the filamentous and heterocystous cyanobacterium Anabaena sp. PCC 7120 with single-cell soft X-ray imaging Reviewed

T Teramoto, M Yoshimura, C Azai, K Terauchi, T Ohta

Journal of Physics: Conference Series   849   012005 - 012005   2017.6

BN-PAGE analysis of cyanobacterial clock protein KaiC Invited Reviewed

Kazuki Terauchi, Katsuaki Oyama, Chihiro Azai

Electrophoresis Letters   61 ( 2 )   107 - 110   2017

Conversion between two conformational states of KaiC is induced by ATP hydrolysis as a trigger for cyanobacterial circadian oscillation Reviewed

Katsuaki Oyama, Chihiro Azai, Kaori Nakamura, Syun Tanaka, Kazuki Terauchi

Scientific Reports   6 ( 1 )   32443   2016.9

Orientations of Iron–Sulfur Clusters F_A and F_B in the Homodimeric Type-I Photosynthetic Reaction Center of Heliobacterium modesticaldum Reviewed

Toru Kondo, Masahiro Matsuoka, Chihiro Azai, Shigeru Itoh, Hirozo Oh-oka

The Journal of Physical Chemistry B   120 ( 18 )   4204 - 4212   2016.5

Nitrogen mapping of the filamentous and heterocystous cyanobacterium Anabaena sp. PCC 7120 cells by soft X-ray microscopy Invited

T. Teramoto, M. Yoshimura, C. Azai, K. Terauchi, H. Namba, T. Ohta

Memoirs of the SR center Ritsumeikan University   18   125 - 126   2016.5

Rethinking about the evolution toward oxygenic photosynthesis and experiments to demonstrate it in the laboratory Invited Reviewed

C. Azai, Y. Tsukatani

News letter of the Japanese Society of Photosynthesis Research   26 ( 1 )   43 - 58   2016.4

Mutation-induced perturbation of the special pair P840 in the homodimeric reaction center in green sulfur bacteria Reviewed

Chihiro Azai, Yuko Sano, Yuki Kato, Takumi Noguchi, Hirozo Oh-oka

Scientific Reports   6 ( 1 )   19878   2016.1

Menaquinone as the Secondary Electron Acceptor in the Type I Homodimeric Photosynthetic Reaction Center of Heliobacterium modesticaldum Reviewed

Toru Kondo, Shigeru Itoh, Masahiro Matsuoka, Chihiro Azai, Hirozo Oh-oka

The Journal of Physical Chemistry B   119 ( 27 )   8480 - 8489   2015.6

Direct observation of nitrogen fixation in filamentous cyanobacteria by using soft X-ray microscopy Invited

T. Teramoto, M. Yoshimura, C. Azai, K. Terauchi, H. Namba, T. Ohta

Memoirs of the SR center Ritsumeikan University   17   147 - 148   2015.5

3P103 Isolation of Rieske/cytochrome b complex from a green sulfur bacterium Chlorobaculum tepidum(03. Membrane proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Oh-oka Hirozo, Yamamoto Kazuya, Mutoh Risa, Azai Chihiro, Kurisu Genji

Seibutsu Butsuri   54 ( 1 )   S266   2014

3P263 Two Disconnected Antenna Chlorophyll Pools in Type-1 Photosynthetic Reaction Center of Green Sulfur Bacteria(Photobiology: Photosynthesis,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Chihiro Azai, Toru Kondo, Shigeru Itoh, Hirozo Oh-oka

Seibutsu Butsuri   54 ( 1 )   S292   2014

1P250 Structural studies on the maturation mechanism of [FeFe] hydrogenase maturation from green alga Chlamydomonas reinhardtii(18B. Photobiology:Photosynthesis,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Kiyota Daiki, Mutoh Risa, Azai Chihiro, Oh-oka Hirozo, Kurisu Genji

Seibutsu Butsuri   54 ( 1 )   S182   2014

Gene Expression System in Green Sulfur Bacteria by Conjugative Plasmid Transfer

Chihiro Azai, Jiro Harada, Hirozo Oh-oka

PLoS ONE   8 ( 11 )   e82345 - e82345   2013.11

Reaction of A₁-menaquinone in Type I Reaction Center of Heliobacterium modesticaldum at Cryogenic Temperature Reviewed

Toru Kondo, Masahiro Matsuoka, Chihiro Azai, Hiroyuki Mino, Hirozo Oh-oka, Shigeru Itoh

Advanced Topics in Science and Technology in China   21 - 24   2013

3PT235 Local Structural Modifications around the Primary Electron Donor of the Green Sulfur Bacterial Photosynthetic Reaction Center(The 50th Annual Meeting of the Biophysical Society of Japan)

Azai Chihiro, Sano Yuko, Noguchi Takumi, Oh-oka Hirozo

Seibutsu Butsuri   52   S181   2012

A heterogeneous tag-attachment to the homodimeric type 1 photosynthetic reaction center core protein in the green sulfur bacterium Chlorobaculum tepidum Reviewed

Chihiro Azai, Kwang Kim, Toru Kondo, Jiro Harada, Shigeru Itoh, Hirozo Oh-oka

Biochimica et Biophysica Acta (BBA) - Bioenergetics   1807 ( 7 )   803 - 812   2011.7

The molecular biological method for construction of artificial heterodimeric photosynthetic reaction centers in green sulfur bacteria

C. Azai, T. Kondo, K. Kim, J. Harada, S. Itoh, H. Oh-oka

2011.7

Crystal Structure of the Electron Carrier Domain of the Reaction Center Cytochrome c_z Subunit from Green Photosynthetic Bacterium Chlorobium tepidum Reviewed

Yu Hirano, Makoto Higuchi, Chihiro Azai, Hirozo Oh-oka, Kunio Miki, Zheng-Yu Wang

Journal of Molecular Biology   397 ( 5 )   1175 - 1187   2010.4

C-type cytochromes in the photosynthetic electron transfer pathways in green sulfur bacteria and heliobacteria Invited Reviewed

Chihiro Azai, Yusuke Tsukatani, Shigeru Itoh, Hirozo Oh-oka

Photosynthesis Research   104 ( 2-3 )   189 - 199   2010.1

Sulfur oxidation in mutants of the photosynthetic green sulfur bacterium Chlorobium tepidum devoid of cytochrome c-554 and SoxB Reviewed

Chihiro Azai, Yusuke Tsukatani, Jiro Harada, Hirozo Oh-oka

Photosynthesis Research   100 ( 2 )   57 - 65   2009.5

3P-223 Spectroscopic characterization of the His-tagged homodimeric photosynthetic reaction center(Photobiology:Photosynthesis,The 47th Annual Meeting of the Biophysical Society of Japan)

Azai Chihiro, Kondo Toru, Harada Jiro, Itoh Shigeru, Oh-oka Hirozo

Seibutsu Butsuri   49   S188   2009

Accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence in the CT2256-deleted mutant of the green sulfur bacterium Chlorobium tepidum Reviewed

Jiro Harada, Shohei Miyago, Tadashi Mizoguchi, Chihiro Azai, Kazuhito Inoue, Hitoshi Tamiaki, Hirozo Oh-Oka

Photochemical & Photobiological Sciences   7 ( 10 )   1179 - 1187   2008.10

Parallel electron donation pathways to cytochrome c_z in the type I homodimeric photosynthetic reaction center complex of Chlorobium tepidum Reviewed

Yusuke Tsukatani, Chihiro Azai, Toru Kondo, Shigeru Itoh, Hirozo Oh-oka

Biochimica et Biophysica Acta (BBA) - Bioenergetics   1777 ( 9 )   1211 - 1217   2008.9

Bifurcated Electron Donations from Quinol Oxidoreductase and Soluble CycA to Cytochrome cz of the Photosynthetic Reaction Center Complex in the Green Sulfur Bacterium Chlorobium tepidum Reviewed

Chihiro Azai, Yusuke Tsukatani, Ryo Miyamoto, Toru Kondo, Hiroumi Murakami, Shigeru Itoh, Hirozo Oh-oka

Photosynthesis. Energy from the Sun   549 - 552   2008

Detection of Quinone Function in the Homodimeric Type-I Reaction Center of Heliobacterium modesticaldum Reviewed

Toru Kondo, Hiroyuki Mino, Masahiro Matsuoka, Chihiro Azai, Hirozo Ohoka, Shigeru Itoh

Photosynthesis. Energy from the Sun   123 - 126   2008

3P-273 Arrangements of the electron transfer cofactor A_1 (menaquinone) molecule in the homodimer reaction center of Heliobacterium modesticaldum(The 46th Annual Meeting of the Biophysical Society of Japan)

Kondo Toru, Matsuoka Masahiro, Azai Chihiro, Yoshioka Tetsunari, Mino Hiroyuki, Oh-oka Hirozo, Itoh Shigeru

Seibutsu Butsuri   48   S169   2008

Electron transfer in the homodimeric reaction center core complex isolated from Heliobacterium modesticaldum Reviewed

Kondo Toru, Miyamoto Ryo, Mino Hiroyuki, Matsuoka Masahiro, Azai Chihiro, Ohoka Hirozo, Itoh Shigeru

Plant and Cell Physiology   48   S126   2007

2P329 Arrangements of the electron transfer cofactors in the homodimer reaction center of Heliobacterium modesticaldum(Photobiology-photosynthesis,Poster Presentations)

Kondo Toru, Matsuoka Masahiro, Azai Chihiro, Miyamoto Ryo, Mino Hiroyuki, Ohoka Hirozo, Itoh Shigeru

Seibutsu Butsuri   47   S195   2007

2P349 Fast electron donation from CycA to the homodimeric type-I reaction center of the green sulfur bacterium Chlorobium tepidum(43. Photosynthesis,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Azai Chihiro, Tsukatani Yusuke, Itoh Shigeru, Oh-oka Hirozo

Seibutsu Butsuri   46 ( 2 )   S383   2006

3P278 Sulfur oxidation pathways linked with photosynthetic electron transfer pathway in the green sulfur bacterium Chlorobium tepidum

Azai C., Tsukatani Y., Itoh S., Oh-oka H.

Seibutsu Butsuri   45   S273   2005

In vitro analysis of the cyanobacterial circadian oscillator composed of three Kai proteins

K. Oyama, C. Azai, K. Terauchi

News letter of the Japanese Society of Photosynthesis Research   25 ( 3 )   175-180 - 180   2015.12

Molecular Construction and Reaction Mechanism of Homodimeric Photosynthetic Reaction Center

C. Azai, H. Oh-oka

BIOINDUSTRY   30 ( 12 )   40-47 - 47   2013.12

Site-directed mutagenesis on the photosynthetic reaction center of green sulfur bacteria

C. Azai, H. Oh-oka

News letter of the Japanese Society of Photosynthesis Research   23 ( 2 )   44-47 - 47   2013.8

Expression systems of exogenous gene in the obligatory anaerobic photosynthetic bacterium Chlorobaculum tepidum

C. Azai, H. Oh-oka

News letter of the Japanese Society of Photosynthesis Research   21 ( 3 )   95-101 - 101   2011.12

The photosynthetic linear electron transfer pathway in green sulfur bacteria

Y. Tsukatani, C. Azai, H. Oh-oka

News letter of the Japanese Society of Photosynthesis Research   20 ( 2 )   100-108 - 108   2010.8

Preparations of membrances from anoxygenic photosynthetic bacteria

Low temperature science   67   205 - 208   2008

Light harvesting mechanism of the smallest type-I reaction center of green sulfur bacteria: mutational modifications and theoretical analysis

Chihiro Azai, Hirozo Oh-oka, Shigeru Itoh, Hirotaka Kito, Akihiro Kimura

The 64th Annual Meeting of the Japanese Society of Plant Physiologists  2023.3 

原核生物のゲノム情報から紐解く光合成の起源と進化 Invited

西原 亜理沙, 塚谷 祐介, 浅井 智広, 延 優

第2回原核光合成生物シンポジウム  ( 東北大学・川内キャンパス（ハイブリッド） )   2023.3 

緑色硫黄細菌で見つかった嫌気的な光エネルギー散逸過程 Invited

浅井 智広

藍藻の分子生物学2022  ( かずさアカデミアホール )   2022.12 

Revisiting the function of special pair chlorophylls in type-1 reaction centers Invited International conference

Chihiro Azai

The International Symposium on Photosynthesis and Chloroplast Regulation  ( Kobe Fashion Mart, Kobe )   2022.11  The Grant-in-Aid for Scientific Research on Innovative Areas, New Photosynthesis

昨日の帰納はキノンの機能：タイプ１光合成反応中心にキノンは要るのか？ Invited

浅井智広

第二回光合成タンパク質勉強会  ( オンライン )   2022.10 

緑色硫黄細菌の光合成反応中心複合体での三重項カロテノイドの生成

浅井 智広, 木田 雅俊, 稲垣 知実, 小澄 大輔

第34回カロテノイド研究談話会  ( 関西学院大学・西宮上ヶ原キャンパス（ハイブリッド） )   2022.9  日本カロテノイド研究会

Charge separation induced by a lower-energy bacteriochlorophyll in the reaction center complex of Heliobacterium modesticaldum International conference

Chihiro Azai, Risa Kojima, Kazuki Hinago, Masatoshi Kida, Tetsuya Yamamoto, Hirozo Oh-oka, Daisuke Kosumi, Yutaka Nagasawa

The 17th International Symposium on Phototrophic Prokaryotes (ISPP2022)  ( ACC Liverpool, Liverpool )   2022.8 

緑色硫黄細菌Ⅰ型反応中心の励起状態の理論計算と主要BChl-a除去実験

伊藤 繁, 木村 明洋, 鬼頭 宏任, 浅井 智広, 大岡 宏造

第12回日本光合成学会年会  ( オンライン )   2022.5  日本光合成学会

緑色硫黄細菌の光合成反応中心複合体の穏和な解体

稲垣 知実, 寺内 一姫, 浅井 智広

第12回日本光合成学会年会  ( オンライン )   2022.5  日本光合成学会

緑色硫黄細菌とメタン生成菌の人工共生系の構築 Invited

浅井 智広

新学術領域研究「革新的光物質変換」最終公開シンポジウム  ( 淡路夢舞台国際会議場（ハイブリッド） )   2022.3  新学術領域研究「革新的光物質変換」総括班

Photosynthetic hydrogen production in green sulfur bacteria

3rd International Solar Fuels Conference & International Conference on Artificial Photosynthesis-2019  2019.11 

Time-Resolved Spectroscopy of Electron Transfer Processes upon Selective Excitation in the Reaction Center Complex from Heliobacterium modesticaldum

3rd International Solar Fuels Conference & International Conference on Artificial Photosynthesis-2019  2019.11 

Energy Transfer Pathways from Carotenoids in The Photosynthetic Reaction Center Complex of Green Sulfur Bacteria

3rd International Solar Fuels Conference & International Conference on Artificial Photosynthesis-2019  2019.11 

Femtosecond Time-Resolved Transient Absorption Spectroscopy of Reaction Center of Green Sulfur Bacteria: Elucidation of Primary Dynamics of Photosynthesis

3rd International Solar Fuels Conference & International Conference on Artificial Photosynthesis-2019  2019.11 

Functional implication of carotenoid glucosides in the anaerobic photosynthesis of green sulfur bacteria

Young meeting of 3rd International Solar Fuels Conference  2019.11 

Anaerobic energy dissipation by carotenoid glycoside in photosynthetic green sulfur bacteria

1st US-Japan Binational Meeting  2019.10 

Improved preparation of the reaction center complex from the green sulfur bacterium Chlorobaculum tepidum

The 57th Annual Meeting of the Biophysical Society of Japan  2019.9 

Carotenoid glycoside quenches bacteriochlorophyll a fluorescence in the photosynthetic reaction center complex of green sulfur bacteria

The 57th Annual Meeting of the Biophysical Society of Japan  2019.9 

Isolation of the Rieske/cytochrome b complex from green sulfur bacteria and interaction of the Rieske protein with cytochrome c-556

The 57th Annual Meeting of the Biophysical Society of Japan  2019.9 

緑色硫黄細菌の光合成反応中心に結合したカロテノイドのアンテナ機能

第33回カロテノイド研究談話会  2019.9 

Functional Consequence of Glycosyl Transfer to Carotenoid in Photosynthesis of Green Sulfur Bacteria

15th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP19)  2019.6 

緑⾊硫⻩細菌のRieske/cytb複合体の精製検討とcyt c-556との相互作⽤部位の解析

第10回 日本光合成学会年会  2019.5 

緑⾊硫⻩細菌から均⼀な反応中⼼複合体標品を得るための⼯夫

第10回 日本光合成学会年会  2019.5 

緑⾊硫⻩細菌反応中⼼の光合成初期過程ダイナミクス

第10回 日本光合成学会年会  2019.5 

酸素によるシアノバクテリア時計タンパク質KaiCのATPase活性阻害

第10回 日本光合成学会年会  2019.5 

光応答性CheAタンパク質の創⽣ 〜異なる２つのクラスのヒスチジンキナーゼのキメラ化〜

第10回 日本光合成学会年会  2019.5 

カロテノイド配糖体エステルの緑⾊硫⻩細菌における光合成機能

第10回 日本光合成学会年会  2019.5 

合成生物学時代のタンパク質の“ファイン”アート：ドメイン構造を超えたヒスチジンキナーゼのキメラ化

圧力が拓く生命科学の新領域「圧力生命科学」研究成果報告会 〜あっと驚くタンパク質研究〜  2019.3 

Primary dynamics of photosynthetic process in the reaction center of green sulfur bacteria

The Chemical Society of Japan  2019.3 

Defect of photosynthetic activity in the cruC gene mutant of the green sulfur bacterium Chlorobaculum tepidum, which lost the ability to synthesize carotenoid glucoside ester

第60回 日本植物生理学会年会  2019.3 

Interaction analysis between the Rieske/cyt b complex and c-type cytochromes in green sulfur bacteria

第60回 日本植物生理学会年会  2019.3 

Enhancement of charge separation in the photosynthetic reaction center of the green sulfur bacterium Chlorobaculum tepidum with external phylloquinones

第60回 日本植物生理学会年会  2019.3 

Creation of blue-light-responsive CheA by fusion of two different-class histidine kinases

第60回 日本植物生理学会年会  2019.3 

Circadian oscillation of cyanobacterial clock protein KaiC under anaerobic conditions

第60回 日本植物生理学会年会  2019.3 

緑色硫黄光合成細菌におけるシアノバクテリア生物時計の細胞内再構成

第13回日本ゲノム微生物学会年会  2019.3 

概日振動発振における時計タンパク質KaiCのC末端領域の機能解析

第13回日本ゲノム微生物学会年会  2019.3 

[FeFe]型ヒドロゲナーゼHydA1 の緑⾊硫⻩細菌Chlorobaculum tepidum細胞内での成熟化に対する鉄イオン添加の促進効果

新学術領域研究「⾰新的光物質変換」第2回公開シンポジウム  2019.1 

緑⾊硫⻩細菌の光合成反応中⼼コアアンテナ系におけるカロテノイド配糖体エステルによる超⾼速消光

新学術領域研究「⾰新的光物質変換」第2回公開シンポジウム  2019.1 

Solubilization and purification of the Rieske/cytochrome b complex in green sulfur bacteria

The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

The orientation of menaquinone in the heliobacterial reaction center analyzed with the EPR spectroscopy

The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

Symmetry or asymmetry? - Site-specific structural modification of the homodimeric photosynthetic reaction center of green sulfur bacteria

The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

X-ray structure of the type-I reaction center from Heliobacterium modesticaldum at 3.2 Å resolution

The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

緑色硫黄細菌Chlorobaculum tepidumのカロテノイド配糖体エステル欠損の光合成活性への影響

第32回カロテノイド研究談話会  2018.9 

Fluorescence lifetime analysis of the green sulfur bacterium Chlorobium tepidum mutant devoid of a reaction center specific carotenoid glycoside

ISPR Conference on Microbial Photosynthesis  2018.8 

Glycolipid and chlorophyll a biosyntheses are required for the heterologous assembly of the type-I photochemical reaction center in purple bacteria

16th International Symposium on Phototrophic Prokaryotes  2018.8 

Reconstitution of circadian oscillation of KaiC from the nonheterocystous nitrogen-fixing cyanobacterium Leptolyngbya boryana

16th International Symposium on Phototrophic Prokaryotes  2018.8 

BciA works as an auxiliary but highly active divinyl reductase in the purple photosynthetic bacterium Rhodobacter capsulatus

16th International Symposium on Phototrophic Prokaryotes  2018.8 

Menaquinone (MQ) in the reaction center of Heliobacterium modesticaldum studied by ESP-EPR signal of P800+MQ–: its implication in the 3D structure

16th International Symposium on Phototrophic Prokaryotes  2018.8 

Creation of phototactic CheA - Chimerization of two different classes of histidine kinases -

14th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP18)  2018.6 

緑色硫黄細菌のRieske/cyt b型シトクロム複合体の可溶化・精製の試み

第9回 日本光合成学会年会  2018.5 

紅色光合成細菌Rhodobactor capsulatusのBciAによる迅速なクロロフィリドaのC8ビニル基還元

第9回 日本光合成学会年会  2018.5 

シアノバクテリアの時計タンパク質KaiCにおけるCII六量体リングのリン酸化と構造変化

第59回 日本植物生理学会年会  2018.3 

異なるクラスのヒスチジンキナーゼを融合した人工青色光受容体タンパク質の作製

第59回 日本植物生理学会年会  2018.3 

光化学系I型反応中心の紅色光合成細菌への導入

第59回 日本植物生理学会年会  2018.3 

軟X線顕微鏡を用いた糸状性シアノバクテリアにおける光合成発生酸素の直接観察

第59回 日本植物生理学会年会  2018.3 

光応答性CheA タンパク質の設計：異なるクラスのヒスチジンキナーゼのキメラ化

第12回日本ゲノム微生物学会年会  2018.3 

Analyses of electron transfer reactions on donor and acceptor sides in heliobacterial photosynthetic reaction center

The 55th Annual Meeting of the Biophysical Society of Japan  2017.9 

Visualization of intracellular element concentration in cyanobacteria with soft x-ray live cell imaging

The 55th Annual Meeting of the Biophysical Society of Japan  2017.9 

Conformational change by phosphorylation and ATP hydrolysis in the cyanobacterial circadian oscillator KaiC

The 55th Annual Meeting of the Biophysical Society of Japan  2017.9 

緑色硫黄細菌Chlorobaculum tepidumの異種遺伝子発現系による、緑藻由来[FeFe]型ヒドロゲナーゼの高発現と水素生産

第8回 日本光合成学会年会  2017.5 

ヘリオバクテリア反応中心コアタンパクとFd1/Fd2の再構成実験

第8回 日本光合成学会年会  2017.5 

分子状酸素の直接観察を目指したシアノバクテリア細胞内の元素分布の軟X線顕微鏡イメージング

第8回 日本光合成学会年会  2017.5 

緑⾊硫⻩光合成細菌におけるシアノバクテリア時計タンパク質の強制発現

第8回 日本光合成学会年会  2017.5 

環境の酸素レベルによるシアノバクテリア⽣物時計再構成系の周期⻑変化

第8回 日本光合成学会年会  2017.5 

シアノバクテリア時計タンパク質KaiC のCIドメインのATPase 活性に依存した2状態変換

第58回 日本植物生理学会年会  2017.3 

軟X線顕微鏡による糸状性シアノバクテリアAnabaena sp. PCC 7120内の窒素分布の可視化

第58回 日本植物生理学会年会  2017.3 

絶対嫌気性の光合成細菌Chlorobaculum tepidumの異種遺伝子発現系を利用した緑藻[FeFe]型ヒドロゲナーゼの細胞内成熟化

第58回 日本植物生理学会年会  2017.3 

Reconstitution experiment of the PshB protein with the photosynthetic reaction center core protein of Heliobacteriaum modesticaldum

第58回 日本植物生理学会年会  2017.3 

N-terminal nucleotide sequence dependency of anaerobic protein overexpression in the photosynthetic green sulfur bacterium Chlorobaculum tepidum

2017 International Conference on Artificial Photosynthesis  2017.3 

窒素固定性シアノバクテリアLeptolyngbya boryanaにおける時計タンパク質KaiCの生化学的解析

第11回 ゲノム微生物学会年会  2017.3 

光合成反応中心複合体に潜む構造機能相関の実験的探索

第9回 BKCバイオインフォマティクス研究会  2017.2 

透過型軟X線顕微鏡によるシアノバクテリアの元素選択的な細胞内構造の観察

PF研究会「PF挿入光源ビームラインBL-19の戦略的利用に関する研究会」  2017.1 

Analyses of transient absorption changes and low-temperature fluorescence in the photosynthetic reaction center of heliobacteria

第54回 日本生物物理学会  2016.11 

Conversion between two hexameric states of KaiC for cyanobacterial circadian oscillation

IGER International Symposium on Biological Rhythms  2016.11 

Circadian Clock in the Nonheterocystous Nitrogen Fixing Cyanobacterium Leptolyngbya boryana

4th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation  2016.10 

シアノバクテリア時計タンパク質KaiCにおけるATP加水分解に伴う構造変換

生物資源研究センター第1回シンポジウム  2016.9 

Determination of Carbon-to-Nitrogen Ratio in the Filamentous and Heterocystous Cyanobacterium Anabaena sp. PCC 7120 with Single-cell Soft X-ray Imaging

13th International Conference on X-ray Microscopy  2016.8 

Functions of Evolutionally Conserved Antenna Chlorophylls in Type-1 Photosynthetic Reaction Centers

Photosynthetic and Respiratory Complexes: From Structure to Function  2016.8 

Mutagenic analysis of conserved antenna chlorophylls in type-1 photosynthetic reaction centers

The 17th International Congress on Photosynthesis Research  2016.8 

Orientations of FA/FB and temperature dependencies of their signal intensities in the homodimeric type 1 reaction center of Heliobacterium modesticaldum

The 17th International Congress on Photosynthesis Research  2016.8 

Structural homology around the special pair chlorophylls among type-1 reaction centers

Satellite Meeting on Photosynthesis  2016.7 

Influence of codon bias on the anaerobic protein overproduction in the phototrophic green sulfur bacterium Chlorobaculum tepidum

Satellite Meeting on Photosynthesis  2016.7 

The conformational change and protomer exchange of KaiC hexamer in the cyanobacterial circadian clock

The 16th Annual Meeting of the Protein Science Society of Japan  2016.6 

ヘリオバクテリア光合成反応中心のエネルギー移動経路に関する解析

第7回 日本光合成学会年会  2016.5 

緑色硫黄細菌での異種遺伝子発現におけるコドン使用頻度の影響

第7回 日本光合成学会年会  2016.5 

ホモダイマー型光合成反応中心の構造と機能：光合成の古くて新しいお話

第16回光科学若手研究会  2016.4 

ヘリオバクテリア光合成反応中心の低温蛍光解析

第57回 日本植物生理学会年会  2016.3 

ヘリオバクテリア由来の1 型光合成反応中心の活性測定

第57回 日本植物生理学会年会  2016.3 

緑色硫黄細菌Chlorobaculum tepidumにおけるGFP 発現の塩基配列依存性

第10回 日本ゲノム微生物学会年会  2016.3 

シアノバクテリア生物時計再構成系における周期長決定機構の解析

第10回 日本ゲノム微生物学会年会  2016.3 

緑藻型[FeFe]ヒドロゲナーゼとH クラスター生合成系の緑色硫黄光合成細菌における嫌気的共発現

第4回公開シンポジウム 新学術領域研究「人工光合成による太陽光エネルギーの物質変換：実用化に向けての異分野融合」  2016.1 

軟Ｘ線顕微鏡による糸状性シアノバクテリアの炭素窒素比の決定

第29回 日本放射光学会年会・放射光科学合同シンポジウム  2016.1 

Stability and spectroscopic characterization of heliobacterial reaction center core protein incorporated into nanoporous silica particles

The 53rd Annual Meeting of the Biophysical Society of Japan  2015.9 

Direct observation of nitrogen fixation in filamentous cyanobacteria by using soft X-ray microscopy

The 53rd Annual Meeting of the Biophysical Society of Japan  2015.9 

Construction of artificial heterodimeric photosynthetic reaction center in the green sulfur bacterium Chlorobaculum tepidum

15th International Symposium on Phototrophic Prokaryotes  2015.8 

Bacteriochlorophyll a synthesis in the green sulfur bacterium Chlorobaculum tepidum mutant lacking BchX, a subunit of chlorophyllide a oxidoreductase

15th International Symposium on Phototrophic Prokaryotes  2015.8 

A Rieske/Cytochrome b Complex in the Green Sufur Bacterium Chlorobaculum tedium

15th International Symposium on Phototrophic Prokaryotes  2015.8 

Correlation between hexameric states and phosphorylation levels of the cyanobacterial circadian clock protein KaiC

15th International Symposium on Phototrophic Prokaryotes  2015.8 

Heliobacterial type-I homodimer reaction center uses menaquinone as the secondary electron acceptor, at a little different location from that of phylloquinone in PSI

15th International Symposium on Phototrophic Prokaryotes  2015.8 

The roles of two structural states of the KaiC hexamer in the cyanobacterial clock protein complex

第15回 日本蛋白質科学会年会  2015.6 

Characterization of C8-vinyl reduction activities of BciA from Rhodobacter capsulatus

11th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP15)  2015.5 

Ion exchange chromatography and gel filtration chromatography analyses of cyanobacterial clock protein KaiA

11th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP15)  2015.5 

Insight into the function of alanine-422 residue of KaiC involved in autonomous synchronization of circadian phosphorylation rhythm

11th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP15)  2015.5 

Temperature compensation of KaiC phosphorylation in cyanobacterial circadian clock: Biochemical analysis of two homologous domains of KaiC

11th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP15)  2015.5 

ホモダイマー型光合成反応中心の分子生物学的な構造機能解析

第6回 日本光合成学会年会およびシンポジウム  2015.5 

ホモダイマー光合成反応中心複合体の人工的ヘテロダイマー化

第62回 日本生化学会近畿支部例会  2015.5 

シアノバクテリア生物時計再構成系におけるKaiCの二状態変化とATPase活性

第62回 日本生化学会近畿支部例会  2015.5 

タイプ1 光合成反応中心の保存されたアンテナクロロフィルの役割

第56回 日本植物生理学会年会  2015.3 

A cytochrome bc complexe is Rieske/cytochrome b-type in photosynthetic green sulfur bacteria

第56回 日本植物生理学会年会  2015.3 

光合成細菌Rhodobacter capsulatusにおけるジビニル還元酵素BciAのC8ビニル基還元活性解析

第56回 日本植物生理学会年会  2015.3 

大腸菌におけるシアノバクテリア時計遺伝子の発現とその制御解析

第9回 日本ゲノム微生物学会年会  2015.3 

緑色硫黄細菌におけるシアノバクテリア時計遺伝子の異種発現

第9回 日本ゲノム微生物学会年会  2015.3 

糸状シアノバクテリアの元素選択的観察

第28回 日本放射光学会年会  2015.1 

Discrimination between Two Structural States of the KaiC Hexamer in the Cyanobacterial Clock Protein Complex

The 3rd International Symposium "Dynamical ordering of biomolecular systems for creation of integrated functions"  2015.1 

Isolation of Rieske/cytochrome b complex from a green sulfur bacterium Chlorobaculum tepidum

The 52nd Annual Meeting of the Biophysical Society of Japan  2014.9 

Two Disconnected Antenna Chlorophyll Pools in Type-1 Photosynthetic Reaction Center of Green Sulfur Bacteria

The 52nd Annual Meeting of the Biophysical Society of Japan  2014.9 

Structural studies of [FeFe] hydrogenase maturation from green alga Chlamydomonas reinhardtii

The 52nd Annual Meeting of the Biophysical Society of Japan  2014.9 

Effect of redox state of KaiA on the cyanobacterial circadian clock in vitro

10th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP14)  2014.5 

ヘリオバクテリアI型反応中心のキノン反応・部位はPSIと異なる

第5回 日本光合成学会年会  2014.5 

シアノバクテリア生物時計再構成系におけるKaiCの周期的構造変化解析

第5回 日本光合成学会年会  2014.5 

緑色硫黄細菌のタイプI型RC はPS Iとは異なるエネルギー移動系をもつ

第5回 日本光合成学会年会  2014.5 

緑色硫黄細菌Chlorobaculum tepidumにおいてbchX遺伝子はバクテリオクロロフィルa生合成に必須ではない

第55回 日本植物生理学会年会  2014.3 

多孔質ガラス中のナノ空間における光化学系IIコア複合体からcyt c6への電子伝達

第55回 日本植物生理学会年会  2014.3 

多孔性シリカ粒子に吸着したヘリオバクテリア反応中心コアタンパクの分光学的諸性質

第55回 日本植物生理学会年会  2014.3 

Site-specific structural modification around the special pair of homodimeric photosynthetic reaction center in green sulfur bacteria

The 16th International Congress on Photosynthesis Research  2013.8 

Studies on the photosynthetic reaction centre from the green sulfur bacterium Chlorobaculum tepidum

The 16th International Congress on Photosynthesis Research  2013.8 

Heliobacterial Reaction Center Core Complex in Nanoporous Silica Material

The 16th International Congress on Photosynthesis Research  2013.8 

Inactivation of BchX, a subunit of chlorophyllide a oxidoreductase, in the green sulfur bacterium Chlorobaculum tepidum

Light Harvesting Satellite Meeting 2013  2013.8 

Studies on the photosynthetic reaction centre from the green sulphur bacterium Chlorobaculum tepidum

Light Harvesting Satellite Meeting 2013  2013.8 

光合成反応中心複合体における非対称な電子移動

CREST有機太陽電池シンポジウム  2013.7 

緑色硫黄細菌の一次電子供与体P840の酸化還元差スペクトルの帰属再検討

第4回 日本光合成学会年会  2013.5 

部位特異的変異導入によるホモダイマ―型光合成反応中心の構造改変

第54回 日本植物生理学会年会  2013.3 

光合成反応中心の構造機能相関の解明に向けた分子生物学的なアプローチ

第1回 立命館大学BKC 生体分子ネットワークセミナー  2013.1 

緑色硫黄細菌のホモダイマ—光合成反応中心において部位特異的変異が一次電子供与体周辺の構造に与える影響

第50回 日本生物物理学会年会  2012.9 

Studies on the reduction processes from chlorophyll a estrified with geranylgeraniol to that with ∆2,6-phytadienol in the green sulfur bacterium Chlorobaculum tepidum

14th International Symposium on Phototrophic Prokaryotes  2012.8 

The gene expression system in the Green Sulfur Bacterium Chlorobaculum tepidum by Conjugative Plasmid Transfer

14th International Symposium on Phototrophic Prokaryotes  2012.8 

緑色硫黄細菌の光合成反応中心の部位特異的変異体：一次電子供与体P840周辺の水素結合構造の改変を例として

第3回 日本光合成学会大会  2012.6 

人工ヘテロダイマー光合成反応中心複合体の作製と精製

第53回 日本植物生理学会年会  2012.3 

The molecular biological method for the construction of artificial heterodimeric photosynthetic reaction center in green sulfur bacteria

The 5th Asia and oceania Conference on Photobiology  2011.8 

絶対嫌気性の光合成細菌Chlorobaculum tepidumの外来遺伝子発現系

第2回 日本光合成学会大会  2011.6 

Reaction of A1-menaquinone in type I reaction center of Heliobacterium modesticaldum at cryogenic temperature

15th International Congress of Photosynthesis (PS2010)  2010.8 

The His-tagged reaction center complex of the green sulfur bacterium Chlorobaculum tepidum

15th International Congress of Photosynthesis (PS2010)  2010.8 

人工的ヘテロダイマー化を可能にするホモダイマー型光合成反応中心への部位特異的変異導入法

第18回 光合成の色素系と反応中心に関するセミナー  2010.7 

コアタンパク質の偽二倍体化によるホモダイマー型光合成反応中心のヘテロダイマー化

第1回 日本光合成学会大会  2010.6 

ホモダイマー型光合成反応中心のアフィニティ精製と分光学的性質

第47回 日本生物物理学会年会  2009.10 

The role of cytochrome c-554 in the electron transfer pathways in the green sulfur bacterium Chlorobium tepidum

13th International Symposium on Phototrophic Prokaryotes  2009.8 

緑色硫黄細菌Chlorobium tepidumの光合成反応中心複合体のアフィニティクロマトグラフィによる調製

第50回 日本植物生理学会年会  2009.3 

緑色硫黄細菌Chlorobium tepidumのホモダイマー型光化学系反応中心への変異導入法の検討

第49回 日本植物生理学会年会  2008.3 

緑色硫黄光合成細菌の電子伝達経路の分岐とその生理的意義

第14回 日本光生物学協会年会  2007.7 

Detection of quinone function in the homodimeric type-I reaction center of Heliobacterium modesticaldum

14th International Congress on Photosynthesis (PS2007)  2007.7 

Bifurcated electron donations from quinol oxidoreductase and soluble CycA to cytochrome cz of the photosynthetic reaction center complex in the green sulfur bacterium Chlorobium tepidum

14th International Congress on Photosynthesis (PS2007)  2007.7 

緑色硫黄細菌における反応中心への競合的な電子供与反応

第15回 光合成の色素系と反応中心に関するセミナー  2007.6 

Fast electron donation from CycA to the homodimeric type-I reaction center of the green sulfur bacteria Chlorobium tepidum

5th East Asian Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan  2006.11 

Involvement of CycA in thiosulfate oxidatiton in the green sulfur bacteria Chlorobium tepidum

12th International Symposium on Phototrophic Prokaryotes  2006.8 

緑色硫黄細菌Chlorobium tepidumの光合成電子伝達経路とリンクした硫黄酸化経路

第43回 日本生物物理学会年会  2005.11 

紅藻オキツノリ着生藻の形態的、生理的特性

第29回 日本藻類学会大会  2005.3 

Development of photosynthetic methane production by hydrogen production in green sulfur bacteria

Grant number：18H05181  2018.4 - 2020.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Ritsumeikan University

Unveiling proton-motive force generation by bacterial type-1 photosynthetic reaction ceters

Grant number：17H05731  2017.4 - 2019.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Ritsumeikan University

Construction of artificial blue light receptor for synthetic phototaxis of purple photosynthetic bacteria

Grant number：16K14907  2016.4 - 2018.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research  Ritsumeikan University

Azai Chihiro, SHIMOJI Mamiko

The mechanisms to control electron transfer reactions in the homodimeric photosynthetic reaction center based on its molecular structure

Grant number：15K07026  2015.4 - 2018.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)  Osaka University

Oh-oka Hirozo, Itoh Shigeru, Kurisu Genji, Azai Chihiro

高い水素発生活性を実現する[FeFe]型ヒドロゲナーゼの構造デザイン

Grant number：15H00886  2015.4 - 2017.3

日本学術振興会  科学研究費助成事業  新学術領域研究(研究領域提案型)  立命館大学

浅井 智広

Functional analysis of quinone in the photosynthetic reaction center complex of green sulfur bacteria

Grant number：25891025  2013.8 - 2015.3

Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity Start-up  Ritsumeikan University

AZAI Chihiro

分子生物学実験

Institution：立命館大学

Experiments in Basic Biology

Institution：Ritsumeikan University

生物学基礎実験

Institution：立命館大学