Updated on 2025/06/06

写真a

 
MUNEYUKI Eiro
 
Organization
Faculty of Science and Engineering Professor
Other responsible organization
Physics Course of Graduate School of Science and Engineering, Master's Program
Physics Course of Graduate School of Science and Engineering, Doctoral Program
Contact information
The inquiry by e-mail is 《here
External link

Degree

  • 理学博士 ( 東京大学 )

  • 理学修士 ( 東京大学 )

Education

  • 1989.3
     

    The University of Tokyo   Graduate School, Division of Science   doctor course   completed

  • 1989.3
     

    The University of Tokyo   Graduate School, Division of Science   doctor course   completed

  • 1986.3
     

    The University of Tokyo   Graduate School, Division of Science   master course   completed

  • 1984.3
     

    The University of Tokyo   Faculty of Science   graduated

  • 1980.3
     

    洛星ヴィアトール学園高等学校   graduated

Research History

  • 2009.4 - 2020.3

    Gakushuin University   理学部物理学科   非常勤講師

  • 2008.4 -  

    Chuo University   理工学部   教授

  • 2007.4 - 2008.3

    Chuo University   理工学部   准教授

  • 2005.4 - 2007.3

    Chuo University   理工学部   助教授

  • 2004.4 - 2005.3

    Waseda University   教育学部   非常勤講師

  • 1990.3 - 2005.3

    Tokyo Institute of Technology   資源化学研究所   助手

  • 1991.4 - 1993.3

    Chiba University   園芸学部   非常勤講師

  • 1989.4 - 1990.3

    Saitama University   工学部   助手

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Professional Memberships

  • 日本生化学会

  • 日本生物物理学会

  • 日本物理学会

Research Interests

  • Biophysics

  • Biological energy transduction

Research Areas

  • Life Science / Biophysics  / 生物物理学

Papers

  • Conformational change mechanisms driving the rotation of F1-ATPase Reviewed

    Masahiro Motohashi, Mao Oide, Chigusa Kobayashi, Jaewoon Jung, Eiro Muneyuki, Yuji Sugita

    BioNano 8   8   2023.6

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  • Tight Chemomechanical Coupling of the F1 Motor Relies on Structural Stability. Reviewed International journal

    Mana Tanaka, Tomohiro Kawakami, Tomoaki Okaniwa, Yohei Nakayama, Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki

    Biophysical journal   119 ( 1 )   48 - 54   2020.7

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    The F1 motor is a rotating molecular motor that ensures a tight chemomechanical coupling between ATP hydrolysis/synthesis reactions and rotation steps. However, the mechanism underlying this tight coupling remains to be elucidated. In this study, we used electrorotation in single-molecule experiments using an F1βE190D mutant to demonstrate that the stall torque was significantly smaller than the wild-type F1, indicating a loose coupling of this mutant, despite showing similar stepping torque as the wild-type. Experiments on the ATPase activity after heat treatment and gel filtration of the α3β3-subcomplex revealed the unstable structure of the βE190D mutant. Our results suggest that the tight chemomechanical coupling of the F1 motor relies on the structural stability of F1. We also discuss the difference between the stepping torque and the stall torque.

    DOI: 10.1016/j.bpj.2020.04.039

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  • Ratchet model of motor proteins and its energetics

    Yohei Nakayama, Eiro Muneyuki

    The Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery   231 - 243   2018.5

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    Language:English   Publishing type:Part of collection (book)   Publisher:Springer Singapore  

    DOI: 10.1007/978-981-10-8459-1_14

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  • Characterization of Conformational Ensembles of Protonated N-glycans in the Gas-Phase Reviewed

    Re S, Watabe S, Nishima W, Muneyuki E, Yamaguchi Y, MacKerell AD Jr, Sugita Y

    Scientific Reports   8   1644   2018.1

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    DOI: 10.1038/s41598-018-20012-0

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  • Simultaneous Formation and Spatial Patterning of ZnO on ITO Surfaces by Local Laser-Induced Generation of Microbubbles in Aqueous Solutions of [Zn(NH3)4]2+ Reviewed

    Sho Fujii, Ryuta Fukano, Yoshihito Hayami, Hiroaki Ozawa, Eiro Muneyuki, Noboru Kitamura, Masa-aki Haga

    ACS Appl. Mater. Interfaces   9   8413 - 8419   2017.2

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  • Crystal structures of the ATP-binding and ADP-release dwells of the V1 rotary motor. Reviewed

    Suzuki K, Mizutani K, Maruyama S, Shimono K, Imai FL, Muneyuki E, Kakinuma Y, Ishizuka-Katsura Y, Shirouzu M, Yokoyama S, Yamato I, Murata T

    Nat Commun.   27 ( 7 )   13235   2016.9

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  • Formation of M-Like Intermediates in Proteorhodopsin in Alkali Solutions (pH >= similar to 8.5) Where the Proton Release Occurs First in Contrast to the Sequence at Lower pH Reviewed

    Jun Tamogami, Keitaro Sato, Sukuna Kurokawa, Takumi Yamada, Toshifumi Nara, Makoto Demura, Seiji Miyauchi, Takashi Kikukawa, Eiro Muneyuki, Naoki Kamo

    BIOCHEMISTRY   55 ( 7 )   1036 - 1048   2016.2

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    DOI: 10.1021/acs.biochem.5b01196

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  • Hidden Entropy Production in F1-ATPase

    Nakayama Yohei, Tanaka Mana, Muneyuki Eiro

    Meeting Abstracts of the Physical Society of Japan   71   2645 - 2645   2016

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    Language:Japanese   Publisher:The Physical Society of Japan  

    DOI: 10.11316/jpsgaiyo.71.2.0_2645

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  • Single molecule thermodynamics of ATP synthesis by F-1-ATPase Reviewed

    Shoichi Toyabe, Eiro Muneyuki

    NEW JOURNAL OF PHYSICS   17   15008   2015.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1088/1367-2630/17/1/015008

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  • Torque Generation ofEnterococcus hiraeV-ATPase Reviewed

    Hiroshi Ueno, Yoshihiro Minagawa, Mayu Hara, Suhaila Rahman, Ichiro Yamato, Eiro Muneyuki, Hiroyuki Noji, Takeshi Murata, Ryota Iino

    Journal of Biological Chemistry   289 ( 45 )   31212 - 31223   2014.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Biochemistry & Molecular Biology (ASBMB)  

    DOI: 10.1074/jbc.m114.598177

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  • CHARMM Force-Fields with Modified Polyphosphate Parameters Allow Stable Simulation of the ATP-Bound Structure of Ca2+-ATPase Reviewed

    Yasuaki Komuro, Suyong Re, Chigusa Kobayashi, Eiro Muneyuki, Yuji Sugita

    JOURNAL OF CHEMICAL THEORY AND COMPUTATION   10 ( 9 )   4133 - 4142   2014.9

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    DOI: 10.1021/ct5004143

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  • 3P154 Rotor-Stator Interactions in V1 and Vo from Enterococcus hirae V-ATPase(Molecular motor,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Hiroshi Ueno, Yoshihiro Minagawa, Mayu Hara, Ichiro Yamato, Hiroyuki Noji, Takeshi Murata, Ryota Iino, Eiro Muneyuki

    Seibutsu Butsuri   54 ( 1 )   S274   2014

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.54.S274_4

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  • 1P105 Molecular dynamics simulations of ATP/ADP bound forms of SR Ca^<2+>-ATPase using CHARMM force field with modified polyphosphate parameters(03. Membrane proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Komuro Yasuaki, Re Suyong, Kobayashi Chigusa, Muneyuki Eiro, Sugita Yuji

    Seibutsu Butsuri   54 ( 1 )   S158   2014

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    DOI: 10.2142/biophys.54.S158_3

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  • Thermodynamic Analyses of Nucleotide Binding to an Isolated Monomeric beta Subunit and the alpha(3)beta(3)gamma Subcomplex of F-1-ATPase Reviewed

    Yohsuke Kikuchi, Yusuke Naka, Hidemitsu Osakabe, Tetsuaki Okamoto, Tomoko Masaike, Hiroshi Ueno, Shoichi Toyabe, Eiro Muneyuki

    BIOPHYSICAL JOURNAL   105 ( 11 )   2541 - 2548   2013.12

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    DOI: 10.1016/j.bpj.2013.10.018

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  • Basic Properties of Rotary Dynamics of the Molecular Motor Enterococcus hirae V-1-ATPase Reviewed

    Yoshihiro Minagawa, Hiroshi Ueno, Mayu Hara, Yoshiko Ishizuka-Katsura, Noboru Ohsawa, Takaho Terada, Mikako Shirouzu, Shigeyuki Yokoyama, Ichiro Yamato, Eiro Muneyuki, Hiroyuki Noji, Takeshi Murata, Ryota Iino

    JOURNAL OF BIOLOGICAL CHEMISTRY   288 ( 45 )   32700 - 32707   2013.11

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    DOI: 10.1074/jbc.M113.506329

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  • Properties of the electrogenic activity of bacteriorhodopsin. Reviewed International journal

    Shizuma Miyazaki, Makoto Matsumoto, Søren Bo Brier, Toshihiro Higaki, Takumi Yamada, Tetsuaki Okamoto, Hiroshi Ueno, Shoichi Toyabe, Eiro Muneyuki

    European biophysics journal : EBJ   42 ( 4 )   257 - 65   2013.4

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    In this study, we analyzed the photoelectric current generated by bacteriorhodopsin adsorbed on a polymer film, "Lumirror" (Muneyuki et al. in FEBS Lett 427:109-114, 1998). We could examine the photoelectric current over a wide range of light intensity and pH values using the same membrane owing to the mechanical and chemical stability of the thin polymer film. We analyzed the photoelectric current by comparison with a simple equivalent electric circuit. Analysis of experimental results obtained at different light intensities suggested that the electromotive force of the bacteriorhodopsin was independent of light intensity. The pH dependence of the photoelectric current suggested that the bacteriorhodopsin could generate a maximum electromotive force at approximately pH 6.

    DOI: 10.1007/s00249-012-0870-0

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  • Free Energy Analysis on the Tom20-Presequence Complex in Solution for Understanding a Dynamic-Equilibrium Model Reviewed

    Komuro Yasuaki, Miyashita Naoyuki, Mori Takaharu, Muneyuki Eiro, Saitoh Takashi, Kohda Daisuke, Sugita Yuji

    BIOPHYSICAL JOURNAL   104 ( 2 )   665A   2013.1

  • 3P314 Conformational analysis of PA-glycans by replica-exchange molecular dynamics simulations(30.Miscellaneous topics,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

    Watanabe Shigehisa, Re Suyong, Muneyuki Eiro, Sugita Yuji

    Seibutsu Butsuri   53 ( 1 )   S264   2013

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    DOI: 10.2142/biophys.53.S264_1

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  • Energetics of the presequence-binding poses in mitochondrial protein import through Tom20. Reviewed

    Komuro Y, Miyashita N, Mori T, Muneyuki E, Saitoh T, Kohda D, Sugita Y

    J Phys Chem B   117 ( 10 )   2864 - 2871   2013

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  • 3P111 Molecular dynamics simulations of SR Ca^<2+>-ATPase using improved ATP force field(03. Membrane proteins,Poster)

    Komuro Yasuaki, Kobayashi Chigusa, Re Suyong, Muneyuki Eiro, Sugita Yuji

    Seibutsu Butsuri   53 ( 1 )   S230   2013

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    DOI: 10.2142/biophys.53.S230_3

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  • Recovery of state-specific potential of molecular motor from single-molecule trajectory Reviewed

    Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki

    EPL   97 ( 4 )   40004   2012.2

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    DOI: 10.1209/0295-5075/97/40004

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  • 1PS022 Effect of external torque on the rotation of TFβ E190D mutant(The 50th Annual Meeting of the Biophysical Society of Japan)

    Kawakami Tomohiro, Toyabe Shoici, Ueno Hiroshi, Kudo Seishi, Muneyuki Eiro

    Seibutsu Butsuri   52   S77 - S78   2012

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    DOI: 10.2142/biophys.52.S77_5

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  • 2PT139 Molecular dynamics simulations of SR Ca2+-pump in the ATP bound forms(The 50th Annual Meeting of the Biophysical Society of Japan)

    Komuro Yasuaki, Kobayashi Chigusa, Muneyuki Eiro, Sugita Yuji

    Seibutsu Butsuri   52   S128   2012

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    DOI: 10.2142/biophys.52.S128_5

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  • Thermodynamic efficiency and mechanochemical coupling of F-1-ATPase Reviewed

    Shoichi Toyabe, Takahiro Watanabe-Nakayama, Tetsuaki Okamoto, Seishi Kudo, Eiro Muneyuki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   108 ( 44 )   17951 - 17956   2011.11

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    DOI: 10.1073/pnas.1106787108

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  • 理解したいということ

    宗行 英朗

    生物物理   51 ( 4 )   159 - 159   2011.7

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    Language:Japanese   Publisher:一般社団法人 日本生物物理学会  

    DOI: 10.2142/biophys.51.159

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  • Fabrication and Placement of a Ring Structure of Nanoparticles by a Laser-Induced Micronanobubble on a Gold Surface Reviewed

    Sho Fujii, Katsuhiko Kanaizuka, Shoichi Toyabe, Katsuaki Kobayashi, Eiro Muneyuki, Masa-aki Haga

    LANGMUIR   27 ( 14 )   8605 - 8610   2011.7

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    DOI: 10.1021/la201616s

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  • Direct Observation of the Myosin Va Recovery Stroke That Contributes to Unidirectional Stepping along Actin Reviewed

    Katsuyuki Shiroguchi, Harvey F. Chin, Diane E. Hannemann, Eiro Muneyuki, Enrique M. De La Cruz, Kazuhiko Kinosita

    PLOS BIOLOGY   9 ( 4 )   e1001031   2011.4

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    DOI: 10.1371/journal.pbio.1001031

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  • 3G1446 Molecular dynamics simulation of outer mitochondrial membrane protein Tom20-presequence complex(3G Protein: Structure 4,The 49th Annual Meeting of the Biophysical Society of Japan)

    Komuro Yasuaki, Mori Takaharu, Muneyuki Eiro, Saitoh Takashi, Kohda Daisuke, Sugita Yuji

    Seibutsu Butsuri   51   S131 - S132   2011

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    DOI: 10.2142/biophys.51.S131_6

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  • 2Q1636 The fast proton release of proteorhodopsin at low pH(Photobiology: Vision & Photoreception 2,The 48th Annual Meeting of the Biophysical Society of Japan)

    Tamogami Jun, Kikukawa Takashi, Shimono Kazumi, Nara Toshifumi, Muneyuki Eiro, Kamo Naoki

    Seibutsu Butsuri   51   S103   2011

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    DOI: 10.2142/biophys.51.S103_4

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  • Experimental demonstration of information-to-energy conversion and validation of the generalized Jarzynski equality Reviewed

    Shoichi Toyabe, Takahiro Sagawa, Masahito Ueda, Eiro Muneyuki, Masaki Sano

    NATURE PHYSICS   6 ( 12 )   988 - 992   2010.12

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    DOI: 10.1038/NPHYS1821

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  • Observation of DNA pinning at laser focal point on Au surface and its application to single DNA nanowire and cross-wire formation Reviewed

    Sho Fujii, Katsuaki Kobayashi, Katsuhiko Kanaizuka, Tetsuaki Okamoto, Shoichi Toyabe, Eiro Muneyuki, Masa-aki Haga

    BIOELECTROCHEMISTRY   80 ( 1 )   26 - 30   2010.11

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    DOI: 10.1016/j.bioelechem.2010.04.004

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  • Nonequilibrium energetics of a single F1-ATPase molecule. Reviewed

    Toyabe S, Okamoto T, Watanabe-Nakayama T, Taketani H, Kudo S, Muneyuki E

    Physical Review Letters   104 ( 19 )   198103   2010.5

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    Publisher:American Physical Society  

    DOI: 10.1103/PhysRevLett.104.198103

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  • Chemo-Mechanical Coupling in F-1-ATPase Revealed by Catalytic Site Occupancy during Catalysis Reviewed

    Rieko Shimo-Kon, Eiro Muneyuki, Hiroshi Sakai, Kengo Adachi, Masasuke Yoshida, Kazuhiko Kinosita

    BIOPHYSICAL JOURNAL   98 ( 7 )   1227 - 1236   2010.4

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    DOI: 10.1016/j.bpj.2009.11.050

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  • Manipulation of Single DNA Using a Micronano Bubble Formed by Local Laser Heating on an Au-coated Surface Reviewed

    Sho Fujii, Katsuaki Kobayashi, Katsuhiko Kanaizuka, Tetsuaki Okamoto, Shoichi Toyabe, Eiro Muneyuki, Masa-aki Haga

    Chemistry Letters   39 ( 2 )   92 - 93   2010.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Chemical Society of Japan  

    We report a method for manipulating sequential single DNA strands by using a micronanobubble formed by local laser heating on a Au surface. DNA strands near the laser focal point were quickly pulled toward the focal point. This DNA pull-in phenomenon can be explained by Marangoni convection due to a micronanobubble generated by laser heating on the Au surface. The thickness of Au film plays a crucial role for absorbing the IR laser light.

    DOI: 10.1246/cl.2010.92

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  • Allosteric model of an ion pump Reviewed

    Eiro Muneyuki, Ken Sekimoto

    PHYSICAL REVIEW E   81 ( 1 )   011137   2010.1

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    DOI: 10.1103/PhysRevE.81.011137

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  • 2P175 Application of Simple Dark-Field Microscopy with High spatiotemporal resolution(The 48th Annual Meeting of the Biophysical Society of Japan)

    Ueno Hiroshi, Iino Ryota, Tabata Kazuhito V., Sakakihara Shouichi, Eiro Muneyuki, Noji Hiroyuki

    Seibutsu Butsuri   50 ( 2 )   S113   2010

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    DOI: 10.2142/biophys.50.S113_2

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  • 2P123 Molecular dynamics simulation of outer mitochondrial membrane protein Tom20-presequence complex(The 48th Annual Meeting of the Biophysical Society of Japan)

    Komuro Yasuaki, Mori Takaharu, Muneyuki Eiro, Saitoh Takashi, Kohda Daisuke, Sugita Yuji

    Seibutsu Butsuri   50 ( 2 )   S103 - S104   2010

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    DOI: 10.2142/biophys.50.S103_6

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  • 1P191 1B1450 Angle distribution analysis of hybrid F1-ATPase indicates another sub-substep rotation between two catalytic dwells(Molecular motor,Oral Presentations,The 48th Annual Meeting of the Biophysical Society of Japan)

    Ariga Takayuki, Muneyuki Eiro, Yoshida Masasuke

    Seibutsu Butsuri   50 ( 2 )   S53   2010

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    DOI: 10.2142/biophys.50.S53_1

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  • Modulation of nucleotide binding to the catalytic sites of thermophilic F-1-ATPase by the epsilon subunit: Implication for the role of the epsilon subunit in ATP synthesis Reviewed

    Taichi Yasuno, Eiro Muneyuki, Masasuke Yoshida, Yasuyuki Kato-Yamada

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   390 ( 2 )   230 - 234   2009.12

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    DOI: 10.1016/j.bbrc.2009.09.092

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  • A tin oxide transparent electrode provides the means for rapid time-resolved pH measurements: application to photoinduced proton transfer of bacteriorhodopsin and proteorhodopsin. Reviewed

    Tamogami J, Kikukawa T, Miyauchi S, Muneyuki E, Kamo N

    Photochemistry and photobiology   85 ( 2 )   578 - 589   2009.3

  • Watching 'ankle' action of myosin V

    Shiroguchi, Katsuyuki, Chin, Harvey, Muneyuki, Eiro, De La Cruz, Enrique M, Kinosita, Kazuhiko, Jr

    BIOPHYSICAL JOURNAL   96 ( 3 )   546A - 546A   2009.2

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  • 1TA4-10 Single molecule kinetic analysis of the temperature sensitive dwell of F1-ATPase(The 47th Annual Meeting of the Biophysical Society of Japan)

    Okamoto Tetsuaki, Toyabe Shouichi, Muneyuki Eiro

    Seibutsu Butsuri   49   S30 - S31   2009

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    DOI: 10.2142/biophys.49.S30_6

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  • 3P-127 Temperature dependence of nucleotide binding to β-subunit of F1-ATPase revealed by fluorescence measurement.(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Naka Yusuke, Muneyuki Eiro, Osakabe Hidemitsu, Masaike Tomoko

    Seibutsu Butsuri   49   S172   2009

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    DOI: 10.2142/biophys.49.S172_4

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  • 2TA4-03 Stall torque of F1-ATPase(The 47th Annual Meeting of the Biophysical Society of Japan)

    Toyabe Shoichi, Watanabe-Nakayama Takahiro, Okamoto Tetsuaki, Kudo Seishi, Muneyuki Eiro

    Seibutsu Butsuri   49   S43   2009

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    DOI: 10.2142/biophys.49.S43_4

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  • 3P-126 Stepping rotation of F1-ATPase at 2 mM ATP in the presence of DMSO (dimethylsulfoxide).(Molecular motor,The 47th Annual Meeting of the Biophysical Society of Japan)

    Nishizuka Kazuhiro, Toyabe Shoiti, Okamoto Tetsuaki, Muneyuki Eiro

    Seibutsu Butsuri   49   S172   2009

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    DOI: 10.2142/biophys.49.S172_3

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  • Temperature dependence of the rotation and hydrolysis activities of F-1-ATPase Reviewed

    Shou Furuike, Kengo Adachi, Naoyoshi Sakaki, Rieko Shimo-Kon, Hiroyasu Itoh, Eiro Muneyuki, Masasuke Yoshida, Kazuhiko Kinosita

    BIOPHYSICAL JOURNAL   95 ( 2 )   761 - 770   2008.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1529/biophysj.107.123307

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  • Effect of external torque on the ATP-driven rotation of F-1-ATPase Reviewed

    Takahiro Watanabe-Nakayama, Shoichi Toyabe, Selshi Kudo, Shigeru Suglyama, Masasuke Yoshida, Eiro Muneyuki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   366 ( 4 )   951 - 957   2008.2

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    DOI: 10.1016/j.bbrc.2007.12.049

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  • 2P-180 The role of the axle in F_1-ATPase revealed by rotation of a mutant containing a twisted γ subunit(The 46th Annual Meeting of the Biophysical Society of Japan)

    Hirakawa Takayuki, Masaike Tomoko, Iai Kentaro, Muneyuki Eiro, Yoshida Masasuke, Nishizaka Takayuki

    Seibutsu Butsuri   48   S103   2008

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    DOI: 10.2142/biophys.48.S103_1

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  • 3P-214 Electrogenic activity of bacteriorhodopsin adsorbed on Lumirror membrane(The 46th Annual Meeting of the Biophysical Society of Japan)

    Muneyuki Eiro, Higaki Toshihiro, Matsumoto Makoto, Yamada Takumi, Okamoto Tetsuaki, Toyabe Shoichi

    Seibutsu Butsuri   48   S160   2008

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.48.S160_5

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  • 3P-162 How F1 catches ATP from the environment at low temperature(The 46th Annual Meeting of the Biophysical Society of Japan)

    Okamoto Tetsuaki, Toyabe Shouichi, Muneyuki Eiro

    Seibutsu Butsuri   48   S152   2008

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    DOI: 10.2142/biophys.48.S152_4

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  • 1P-167 Three steps of GT mutant (the mutant of F_1) at high ATP concentrations(The 46th Annual Meeting of the Biophysical Society of Japan)

    Taketani Hiroshi, Watanabe-Nakayama Takahiro, Okamoto Tetsuaki, Toyabe Shoichi, Muneyuki Eiro

    Seibutsu Butsuri   48   S47   2008

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    DOI: 10.2142/biophys.48.S47_3

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  • 1P-154 Watching 'ankle' action of myosin V in the absence of actin(The 46th Annual Meeting of the Biophysical Society of Japan)

    Shiroguchi Katsuyuki, Chin Harvey, Muneyuki Eiro, De La Cruz Enrique M., Kinosita Jr.

    Seibutsu Butsuri   48   S45   2008

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    DOI: 10.2142/biophys.48.S45_2

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  • 2P-310 Measurement of Violation of Fluctuation Dissipation Theorem of Single Molecule F_1-ATPase(The 46th Annual Meeting of the Biophysical Society of Japan)

    Toyabe Shoichi, Watanabe-Nakayama Takahiro, Okamoto Tetsuaki, Taketani Hiroshi, Kudo Seishi, Sugiyama Shigeru, Muneyuki Eiro

    Seibutsu Butsuri   48   S122   2008

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    DOI: 10.2142/biophys.48.S122_7

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  • 1P-163 Asymmetric rotation of the hybrid F_1 containing one mutant a subunit(The 46th Annual Meeting of the Biophysical Society of Japan)

    Ariga Takayuki, Muneyuki Eiro, Yoshida Masasuke

    Seibutsu Butsuri   48   S46   2008

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    DOI: 10.2142/biophys.48.S46_5

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  • 3P-156 Temperature dependency of the nucleotide binding to F1-ATPase(The 46th Annual Meeting of the Biophysical Society of Japan)

    Osakabe Hidemitsu, Toyabe Shoichi, Okamoto Tetuaki, Muneyuki Eiro

    Seibutsu Butsuri   48   S151   2008

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    DOI: 10.2142/biophys.48.S151_5

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  • F-1-ATPase rotates by an asymmetric, sequential mechanism using all three catalytic subunits Reviewed

    Takayuki Ariga, Eiro Muneyuki, Masasuke Yoshida

    NATURE STRUCTURAL & MOLECULAR BIOLOGY   14 ( 9 )   841 - 846   2007.9

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    DOI: 10.1038/nsmb1296

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  • カーボンナノチューブ表面で自己組織的に生ずるポリベプチドαヘリックス構造のβシート構造転移--AFMおよびTEMによる解析

    杉山 幸宏, 井上 雄嗣, 宗行 英朗, 羽田 肇, 藤本 正之

    静岡大学大学院電子科学研究科研究報告   28 ( 28 )   53 - 57   2007.3

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  • Single molecule energetics of F-1-ATPase motor Reviewed

    Eiro Muneyuki, Takahiro Watanabe-Nakayama, Tetsuya Suzuki, Masasuke Yoshida, Takayuki Nishizaka, Hiroyuki Noji

    BIOPHYSICAL JOURNAL   92 ( 5 )   1806 - 1812   2007.3

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    DOI: 10.1529/biophysj.106.097170

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  • 3P241 Photo-induced proton transfer of proteorhodopsin (PR) measured by a SnO_2 transparent electrode(Photobiology- vision and photoreception,Poster Presentations)

    Tamogami Jun, Kikukawa Takashi, Miyauchi Seiji, Muneyuki Eiro, Kamo Naoki

    Seibutsu Butsuri   47   S263   2007

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    DOI: 10.2142/biophys.47.S263_2

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  • 3P157 Catalytic Site-Occupancy during ATP Hydrolysis of F_1-ATPase That Lacks Non-Catalytic Nucleotide Binding Site(Molecular motors,Poster Presentations)

    Shimo-Kon Rieko, Muneyuki Eiro, Adachi Kengo, Furuike Shou, Sakai Hiroshi, Yoshida Masasuke, Kinoshita Kazuhiko

    Seibutsu Butsuri   47   S242   2007

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    DOI: 10.2142/biophys.47.S242_2

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  • 3P156 F_1-ATPase rotates by an asymmetric, sequential three-site mechanism(Molecular motors,Oral Presentations)

    Ariga Takayuki, Muneyuki Eiro, Yoshida Masasuke

    Seibutsu Butsuri   47   S242   2007

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    DOI: 10.2142/biophys.47.S242_1

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  • AFM and TEM observations of alpha-helix to beta-sheet conformational change occurring on carbon nanotubes Reviewed

    Yukihiro Sugiyama, Yuji Inoue, Eiro Muneyuki, Hajime Haneda, Masayuki Fujimoto

    JOURNAL OF ELECTRON MICROSCOPY   55 ( 3 )   143 - 149   2006.6

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    DOI: 10.1093/jmicro/dfl024

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  • Kinetic mechanism of quinol oxidation by cytochrome bd studied with ubiquinone-2 analogs Reviewed

    Y Matsumoto, E Muneyuki, D Fujita, K Sakamoto, H Miyoshi, M Yoshida, T Mogi

    JOURNAL OF BIOCHEMISTRY   139 ( 4 )   779 - 788   2006.4

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    DOI: 10.1093/jb/mvj087

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  • An alternative reaction pathway of F-1-ATPase suggested by rotation without 80 degrees/40 degrees substeps of a sluggish mutant at low ATP Reviewed

    K Shimabukuro, E Muneyuki, M Yoshida

    BIOPHYSICAL JOURNAL   90 ( 3 )   1028 - 1032   2006.2

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  • 2P193 Characterization of interaction between β- and γ-subunit in F_1-ATPase using twisted γ mutant(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Shibano Satoko, Muneyuki Eiro, Masaike Tomoko, Okada Kaoru, Yoshida Masasuke, Nishizaka Takayuki

    Seibutsu Butsuri   46 ( 2 )   S344   2006

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    DOI: 10.2142/biophys.46.S344_1

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  • 1P284 The behavior of F_1 motor in the presense of the uniform external torque applied with electrorotation(9. Molecular motor (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

    Nakayama-Watanabe Takahiro, Muneyuki Eiro, Kudo Seishi, Sugiyama Shigeru, Yoshida Masasuke

    Seibutsu Butsuri   46 ( 2 )   S217   2006

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    DOI: 10.2142/biophys.46.S217_4

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  • Rotation of F1-ATPase Reviewed

    Eiro Muneyuki, Masasuke Yoshida

    Handbook of ATPases: Biochemistry, Cell Biology, Pathophysiology   261 - 287   2005.12

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    DOI: 10.1002/3527606122.ch10

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  • One rotary mechanism for F1-ATPase over ATP concentrations from millimolar down to nanomolar Reviewed

    N Sakaki, R Shimo-Kon, K Adachi, H Itoh, S Furuike, E Muneyuki, M Yoshida, K Kinosita

    BIOPHYSICAL JOURNAL   88 ( 3 )   2047 - 2056   2005.3

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    DOI: 10.1529/biophysj.104.054668

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  • The characteristics of the (alpha(VC)-C-371)(3)(beta(RC)-C-337)(3 gamma) double mutant subcomplex of the TF1-ATPase indicate that the catalytic site at the alpha(TP)-beta(TP) interface with bound MgADP in crystal structures of MF1 represents a catalytic site containing inhibitory MgADP Reviewed

    S Bandyopadhyay, E Muneyuki, WS Allison

    BIOCHEMISTRY   44 ( 7 )   2441 - 2448   2005.2

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    DOI: 10.1021/bi047694z

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  • 2SA05Ratchet model, single molecule observation of F_1 motor, and energetics

    Muneyuki E.

    Seibutsu Butsuri   45   S18   2005

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    DOI: 10.2142/biophys.45.S18_2

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  • 3P166 A mechanism for the ATPase activity of F_1-ATPase that lacks non catalytic sites

    Shimo-Kon R., Muneyuki E., Sakaki N., Adachi K., Fruike S., Sakai H., Yoshida M., Kinosita K. Jr.

    Seibutsu Butsuri   45   S245   2005

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    DOI: 10.2142/biophys.45.S245_2

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  • Chemomechanical coupling in F-1-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation Reviewed

    T Nishizaka, K Oiwa, H Noji, S Kimura, E Muneyuki, M Yoshida, K Kinosita

    NATURE STRUCTURAL & MOLECULAR BIOLOGY   11 ( 2 )   142 - 148   2004.2

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    DOI: 10.1038/nsmb721

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  • Rotation of F-1-ATPase at very low ATP concentrations

    N Sakaki, R Shimo-Kon, H Itoh, K Adachi, S Furuike, E Muneyuki, M Yoshida, K Kinosita

    BIOPHYSICAL JOURNAL   86 ( 1 )   476A - 476A   2004.1

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  • 3P147 Site occupancy and ATP hydrolysis activity of F_1-ATPase without noncatalytic nucleotide binding site

    Shimo-Kon R., Muneyuki E., Sakaki N., Adachi K., Furuike S., Itoh H., Yoshida M., Kinosita Jr. K.

    Seibutsu Butsuri   44   S226   2004

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    DOI: 10.2142/biophys.44.S226_3

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  • 3P148 Single molecule analysis of the rotation in F_1-ATPase mutant: the effect of ATP-binding dwell length on the catatytic dwell

    Shimabukuro K., Muneyuki E., Yoshida M.

    Seibutsu Butsuri   44   S226   2004

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    DOI: 10.2142/biophys.44.S226_4

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  • 3P145 Rotation and torque of F_1-ATPase at extremely low ATP concentrations

    Sakaki N., Shimo-Kon R., Itoh H., Adachi K., Muneyuki E., Yoshida M., Kinosita Jr. K., Furuike S.

    Seibutsu Butsuri   44   S226   2004

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    DOI: 10.2142/biophys.44.S226_1

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  • Catalysis and rotation of F-1 motor: Cleavage of ATP at the catalytic site occurs in 1 ms before 40 degrees substep rotation Reviewed

    K Shimabukuro, R Yasuda, E Muneyuki, KY Hara, K Kinosita, M Yoshida

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   100 ( 25 )   14731 - 14736   2003.12

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    DOI: 10.1073/pnas.2434983100

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  • Origin of apparent negative cooperativity of F-1-ATPase Reviewed

    S Ono, KY Hara, J Hirao, B Matsui, H Noji, M Yoshida, E Muneyuki

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   1607 ( 1 )   35 - 44   2003.10

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    DOI: 10.1016/j.bbabio.2003.08.006

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  • Evidence for rotation of V-1-ATPase Reviewed

    H Imamura, M Nakano, H Noji, E Muneyuki, S Ohkuma, M Yoshida, K Yokoyama

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   100 ( 5 )   2312 - 2315   2003.3

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    DOI: 10.1073/pnas.0436796100

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  • Measurement of site occupancy and rotation speed of F1-ATPase without noncatalytic nucleotide binding site

    Kon-Shimo R., Muneyuki E., Iai K., Sakaki N., Adachi K., Itoh H., Furuike S., Maki Y., Yoshida M., Kinosita Jr. K.

    Seibutsu Butsuri   43   S134   2003

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    DOI: 10.2142/biophys.43.S134_2

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  • Relationship between the free energy of ATP hydrolysis and step rotation of F_1-ATPase

    Muneyuki E., Watanabe T., Noji H., Nishizaka T., Yoshida M.

    Seibutsu Butsuri   43   S134   2003

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    DOI: 10.2142/biophys.43.S134_3

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  • Analysis of rotation mechanism of F_1-ATPase using twisted γ mutant

    Iai K., Muneyuki E., Yoshida M.

    Seibutsu Butsuri   43   S134   2003

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    DOI: 10.2142/biophys.43.S134_4

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  • Single molecule analysis of cooperative rotation mechanism using hybrid F_1-ATPase

    Ariga T., Shimabukura K., Muneyuki E., Yoshida M.

    Seibutsu Butsuri   43   S133   2003

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    DOI: 10.2142/biophys.43.S133_3

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  • Cl- concentration dependence of photovoltage generation by halorhodopsin from Halobacterium salinarum Reviewed

    E Muneyuki, C Shibazaki, Y Wada, M Yakushizin, H Ohtani

    BIOPHYSICAL JOURNAL   83 ( 4 )   1749 - 1759   2002.10

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  • F1-ATPase changes its conformations upon phosphate release. Reviewed International journal

    Masaike,T, Muneyuki,E, Noji,H, Kinosita,K.Jr, Yoshida,M

    J.Biol.Chem.   277 ( 24 )   21643 - 21649   2002.6

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    Motor proteins, myosin, and kinesin have gamma-phosphate sensors in the switch II loop that play key roles in conformational changes that support motility. Here we report that a rotary motor, F1-ATPase, also changes its conformations upon phosphate release. The tryptophan mutation was introduced into Arg-333 in the beta subunit of F1-ATPase from thermophilic Bacillus PS3 as a probe of conformational changes. This residue interacts with the switch II loop (residues 308-315) of the beta subunit in a nucleotide-bound conformation. The addition of ATP to the mutant F1 subcomplex alpha3beta(R333W)3gamma caused transient increase and subsequent decay of the Trp fluorescence. The increase was caused by conformational changes on ATP binding. The rate of decay agreed well with that of phosphate release monitored by phosphate-binding protein assays. This is the first evidence that the beta subunit changes its conformation upon phosphate release, which may share a common mechanism of exerting motility with other motor proteins.

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  • The presence of phosphate at a catalytic site suppresses the formation of the MgADP-inhibited form of F-1-ATPase Reviewed

    N Mitome, S Ono, T Suzuki, K Shimabukuro, E Muneyuki, M Yoshida

    EUROPEAN JOURNAL OF BIOCHEMISTRY   269 ( 1 )   53 - 60   2002.1

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  • 2O1515 Torque measurement of F1-motor using magnetic tweezers

    Noji H., Itoh H., Muneyuki E., Adachi K., Yoshida M., Kinoshita K.

    Seibutsu Butsuri   42 ( 2 )   S146   2002

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    DOI: 10.2142/biophys.42.S146_2

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  • 2O1400 A mutant F1-ATPase resistant to ADP inhibition (2)

    Muneyuki E., Suzuki T., Iai K., Noji H., Nishizaka T., Yoshida M

    Seibutsu Butsuri   42 ( 2 )   S145   2002

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    DOI: 10.2142/biophys.42.S145_1

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  • 2O1500 Rotation analysis of F_1-ATPase mutant αзβ(E190D)зγ

    Shimabukuro K., Muneyuki E., Yoshida M.

    Seibutsu Butsuri   42 ( 2 )   S146   2002

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    DOI: 10.2142/biophys.42.S146_1

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  • 2O1330 Origin of substeps in 120°-stepping rotation of F_1-ATPase

    Nishizaka T., Oiwa K., Kimura S., Kinosita K., Yoshida M., Muneyuki E., Noji H.

    Seibutsu Butsuri   42 ( 2 )   S144   2002

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    DOI: 10.2142/biophys.42.S144_3

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  • 2O1445 Rotation and site occupancy of F_1-ATPase at low ATP concentrations

    Sakaki N., Shimo R., Itoh H., Adachi K., Muneyuki E., Yoshida M., Kinosita K.

    Seibutsu Butsuri   42 ( 2 )   S145   2002

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    DOI: 10.2142/biophys.42.S145_4

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  • ATP synthase - A marvellous rotary engine of the cell Reviewed

    M Yoshida, E Muneyuki, T Hisabori

    NATURE REVIEWS MOLECULAR CELL BIOLOGY   2 ( 9 )   669 - 677   2001.9

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  • Alkane derivative-bacteriorhodopsin interaction: proton transport and protein structure Reviewed

    A Shibata, H Ikema, S Ueno, E Muneyuki, T Higuti

    COLLOIDS AND SURFACES B-BIOINTERFACES   22 ( 1 )   31 - 38   2001.9

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  • <視点/動く>分子が動く--新しい機能性材料アプローチ:熱ゆらぎの中で働くナノ分子モーター

    宗行 英朗, 吉田 賢右

    未来材料   1 ( 5 )   2 - 5   2001.5

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  • Pause and rotation of F1-ATPase during catalysis Reviewed

    Yoko Hirono-Hara, Hiroyuki Noji, Masaya Nishiura, Eiro Muneyuki, Kiyotaka Y.Hara, Ryohei Yasuda, Kazuhiko Kinosita,Jr, Masasuke Yoshida

    Proc.Natl. Acad.Sci.USA   98 ( 24 )   13649 - 13654   2001.1

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    DOI: 10.1073/pnas.241365698

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  • Rotation analysis of beta(E190D)F1-ATPase mutant.

    Shimabukuro K., Muneyuki E., Yasuda R., Hara K.Y., Kinosita K., Yoshida M.

    Seibutsu Butsuri   41   S199   2001

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    DOI: 10.2142/biophys.41.S199_1

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  • Expression of the proton pump activity of bacteriorhodopsin with hydrophobic cations : the effect of tertiary amine derivatives

    Yorimitsu A., Shibata A., Ueno S., Higuchi T., Muneyuki E., Kamo N.

    Seibutsu Butsuri   41   S62   2001

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    DOI: 10.2142/biophys.41.S62_2

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  • Analysis of ADP-Mg inhibition of F1-ATPase

    Hirono-Hara Y, Noji H, Muneyuki E, Kinosita K Jr, Yoshida M

    Seibutsu Butsuri   41   S198   2001

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    DOI: 10.2142/biophys.41.S198_3

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  • Direct measurement of rotary potentials of F_1 motor using magnetic tweezers

    Noji H., Itoh H., Shio M., Adachi K., Muneyuki E., Yoshida M., Kinosita K.Jr..

    Seibutsu Butsuri   41   S199   2001

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    DOI: 10.2142/biophys.41.S199_4

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  • Kinetic analysis about MgADP inhibition of F1-ATPase

    Kawashima K., Muneyuki E., Kinosita K.Jr

    Seibutsu Butsuri   41   S198   2001

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    DOI: 10.2142/biophys.41.S198_2

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  • F0F1-ATP synthase: general structural features of 'ATP-engine' and a problem on free energy transduction Reviewed

    E Muneyuki, H Noji, T Amano, T Masaike, M Yoshida

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   1458 ( 2-3 )   467 - 481   2000.5

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  • Properties of the stochastic energization-relaxation channel model for vectorial ion transport Reviewed

    E Muneyuki, TA Fukami

    BIOPHYSICAL JOURNAL   78 ( 3 )   1166 - 1175   2000.3

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  • Rotation of F-1-ATPase and the hinge residues of the beta subunit Reviewed

    T Masaike, N Mitome, H Noji, E Muneyuki, R Yasuda, K Kinosita, M Yoshida

    JOURNAL OF EXPERIMENTAL BIOLOGY   203 ( 1 )   1 - 8   2000.1

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  • A stochastic channel model for free energy transduction

    Muneyuki E., Fukami T.A.

    Seibutsu Butsuri   40   S208   2000

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    DOI: 10.2142/biophys.40.S208_3

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  • alpha(3)beta(3)gamma complex of F-1-ATPase from thermophilic Bacillus PS3 can maintain steady-state ATP hydrolysis activity depending on the number of non-catalytic sites Reviewed

    T Amano, T Matsui, E Muneyuki, H Noji, K Hara, M Yoshida, T Hisabori

    BIOCHEMICAL JOURNAL   343 ( 1 )   135 - 138   1999.10

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  • The noncatalytic site-deficient alpha(3)beta(3)gamma subcomplex and F0F1-ATP synthase can continuously catalyse ATP hydrolysis when P-i is present Reviewed

    D Bald, E Muneyuki, T Amano, J Kruip, T Hisabori, M Yoshida

    EUROPEAN JOURNAL OF BIOCHEMISTRY   262 ( 2 )   563 - 568   1999.6

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  • Chloride concentration dependency of the electrogenic activity of halorhodopsin Reviewed

    D Okuno, M Asaumi, E Muneyuki

    BIOCHEMISTRY   38 ( 17 )   5422 - 5429   1999.4

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  • Cross-linking of two beta subunits in the closed conformation in F-1-ATPase Reviewed

    SP Tsunoda, E Muneyuki, T Amano, M Yoshida, H Noji

    JOURNAL OF BIOLOGICAL CHEMISTRY   274 ( 9 )   5701 - 5706   1999.2

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  • Time-resolved measurements of photovoltage generation by bacteriorhodopsin and halorhodopsin adsorbed on a thin polymer film Reviewed

    E Muneyuki, C Shibazaki, H Ohtani, D Okuno, M Asaumi, T Mogi

    JOURNAL OF BIOCHEMISTRY   125 ( 2 )   270 - 276   1999.2

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  • Direct observation of the rotation of the mutant F1-ATPase without noncatalytic binding site

    Hirono Y., Nishiura M., Muneyuki E., Noji H., Kinoshita K., Yoshida M.

    Seibutsu Butsuri   39   S145   1999

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    DOI: 10.2142/biophys.39.S145_4

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  • Crystallization of F1-ATPase α_3β_3γ sub-assembly

    Shirakihara Y., Kambara M., Amano T., Tsunoda S., Muneyuki E., Shirakihara C., Shiratori A., Yoshida M.

    Seibutsu Butsuri   39   S104   1999

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    DOI: 10.2142/biophys.39.S104_4

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  • Excitation wavelength dependence of the fluorescence spectra of bacteriorhodopsin and halorhodopsin.

    Ohtani H., Muneyuki E.

    Seibutsu Butsuri   39   S73   1999

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    DOI: 10.2142/biophys.39.S73_1

    CiNii Books

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  • V-ATPase from Thermus thermophilus is inactivated during ATP hydrolysis but can synthesis ATP. Reviewed

    Yokoyama, K, Muneyuki, E, Amano, T, Mizutani, S, Yoshida, M, Ohkuma, S

    The Journal of Biological Chemistry   273   12248 - 12252   1998.11

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    DOI: 10.1074/jbc.273.32.20504

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  • V-ATPase of Thermus thermophilus is inactivated during ATP hydrolysis but can synthesize ATP Reviewed

    K Yokoyama, E Muneyuki, T Amano, S Mizutani, M Yoshida, M Ishida, S Ohkuma

    JOURNAL OF BIOLOGICAL CHEMISTRY   273 ( 32 )   20504 - 20510   1998.8

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  • A new system for the measurement of electrogenicity produced by ion pumps using a thin polymer film: examination of wild type bacteriorhodopsin and the D96N mutant over a wide pH range Reviewed

    E Muneyuki, D Okuno, M Yoshida, A Ikai, H Arakawa

    FEBS LETTERS   427 ( 1 )   109 - 114   1998.5

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  • ATP synthesis by F0F1-ATP synthase independent of noncatalytic nucleotide binding sites and insensitive to azide inhibition Reviewed

    D Bald, T Amano, E Muneyuki, B Pitard, JL Rigaud, J Kruip, T Hisabori, M Yoshida, M Shibata

    JOURNAL OF BIOLOGICAL CHEMISTRY   273 ( 2 )   865 - 870   1998.1

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  • Thermophilic F-1-ATPase is activated without dissociation of an endogenous inhibitor, epsilon subunit Reviewed

    Y Kato, T Matsui, N Tanaka, E Muneyuki, T Hisabori, M Yoshida

    JOURNAL OF BIOLOGICAL CHEMISTRY   272 ( 40 )   24906 - 24912   1997.10

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  • A single mutation at the catalytic site of TF1-alpha(3)beta(3)gamma complex switches the kinetics of ATP hydrolysis from negative to positive cooperativity Reviewed

    E Muneyuki, M Odaka, M Yoshida

    FEBS LETTERS   413 ( 1 )   55 - 59   1997.8

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  • Catalytic activity of the alpha(3)beta(3)gamma complex of F-1-ATPase without noncatalytic nucleotide binding site Reviewed

    T Matsui, E Muneyuki, M Honda, WS Allison, C Dou, M Yoshida

    JOURNAL OF BIOLOGICAL CHEMISTRY   272 ( 13 )   8215 - 8221   1997.3

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  • The heterogeneous interaction of substoichiometric TNP-ATP and F-1-ATPase from Escherichia coli Reviewed

    E Muneyuki, T Hisabori, T Sasayama, K Mochizuki, M Yoshida

    JOURNAL OF BIOCHEMISTRY   120 ( 5 )   940 - 945   1996.11

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  • ΔμH+ Dependency of proton translocation by bacteriorhodopsin and a stochastic energization-relaxation channel model Reviewed

    Muneyuki,E, Ikematsu,M, Yoshida,M

    J.Phys.Chem.   100 ( 50 )   19687 - 19691   1996.8

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  • Catalytic activities of alpha(3)beta(3)gamma complexes of F-1-ATPase with 1, 2, or 3 incompetent catalytic sites Reviewed

    T Amano, T Hisabori, E Muneyuki, M Yoshida

    JOURNAL OF BIOLOGICAL CHEMISTRY   271 ( 30 )   18128 - 18133   1996.7

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  • The alpha(3)beta(3)gamma complex of the F-1-ATPase from thermophilic Bacillus PS3 containing the alpha D261N substitution fails to dissociate inhibitory MgADP from a catalytic site when ATP binds to noncatalytic sites Reviewed

    JM Jault, T Matsui, FM Jault, C Kaibara, E Muneyuki, M Yoshida, Y Kagawa, WS Allison

    BIOCHEMISTRY   34 ( 50 )   16412 - 16418   1995.12

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  • ANALYSIS OF TIME-DEPENDENT CHANGE OF ESCHERICHIA-COLI F1-ATPASE ACTIVITY AND ITS RELATIONSHIP WITH APPARENT NEGATIVE COOPERATIVITY Reviewed

    Y KATO, T SASAYAMA, E MUNEYUKI, M YOSHIDA

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   1231 ( 3 )   275 - 281   1995.10

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  • Direct reconstitution of bacteriorhodopsin into planar phospholipid bilayers - detergent effect Reviewed

    Mineo Ikematsu, Yukihiro Sugiyama, Masahiro Iseki, Eiro Muneyuki, Atsuo Mizukami

    Biophysical Chemistry   54 ( 2 )   155 - 164   1995

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    DOI: 10.1016/0301-4622(94)00111-V

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  • CATALYTIC COOPERATIVITY OF BEEF-HEART MITOCHONDRIAL F1-ATPASE REVEALED BY USING 2',3'-O-(2,4,6-TRINITROPHENYL)-ATP AS A SUBSTRATE - AN INDICATION OF MUTUALLY ACTIVATING CATALYTIC SITES Reviewed

    E MUNEYUKI, T HISABORI, WS ALLISON, JM JAULT, T SASAYAMA, M YOSHIDA

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   1188 ( 1-2 )   108 - 116   1994.11

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  • TYR-341 OF THE BETA-SUBUNIT IS A MAJOR K-M-DETERMINING RESIDUE OF TF1-ATPASE - PARALLEL EFFECT OF ITS MUTATIONS ON K-D((ATP)) OF THE BETA-SUBUNIT AND ON K-M((ATP)) OF THE ALPHA(3)BETA(3)GAMMA COMPLEX Reviewed

    M ODAKA, C KAIBARA, T AMANO, T MATSUI, E MUNEYUKI, K OGASAHARA, K YUTANI, M YOSHIDA

    JOURNAL OF BIOCHEMISTRY   115 ( 4 )   789 - 796   1994.4

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  • COMPARISON OF THE ATPASE ACTIVITIES OF BOVINE HEART AND LIVER MITOCHONDRIAL ATP SYNTHASES WITH DIFFERENT TISSUE-SPECIFIC GAMMA-SUBUNIT ISOFORMS Reviewed

    C MATSUDA, E MUNEYUKI, H ENDO, M YOSHIDA, Y KAGAWA

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   200 ( 2 )   671 - 678   1994.4

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  • Direct transmembraneous reconsitution of Bacteriorhodopsin into planar phospholipid bilayers Reviewed

    Muneyuki,E, Ikematsu,M, Iseki,M, Sugiyama,Y, Mizukami,A, Ohno,K, Yoshida,M, Hirata,H

    Biochim.Biophys.Acta   1183   171 - 179   1993.8

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  • INHIBITORY EFFECT OF NAN3 ON THE F0F1 ATPASE OF SUBMITOCHONDRIAL PARTICLES AS RELATED TO NUCLEOTIDE-BINDING Reviewed

    E MUNEYUKI, M MAKINO, H KAMATA, Y KAGAWA, M YOSHIDA, H HIRATA

    BIOCHIMICA ET BIOPHYSICA ACTA   1144 ( 1 )   62 - 68   1993.8

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  • A chaperonin from a thermophilic bacterium, Thermus thermophilus. Reviewed

    M. Yoshida, N. Ishii, E. Muneyuki, H. Taguchi

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences   339 ( 1289 )   305 - 312   1993.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Royal Society(UK,London)  

    DOI: 10.1098/rstb.1993.0029

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  • SINGLE SITE HYDROLYSIS OF 2',3'-O-(2,4,6-TRINITROPHENYL)-ATP BY THE F1-ATPASE FROM THE THERMOPHYLIC BACTERIUM PS3 IS ACCELERATED BY THE CHASE-ADDITION OF EXCESS ATP Reviewed

    T HISABORI, E MUNEYUKI, M ODAKA, K YOKOYAMA, K MOCHIZUKI, M YOSHIDA

    JOURNAL OF BIOLOGICAL CHEMISTRY   267 ( 7 )   4551 - 4556   1992.3

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  • HETEROGENEOUS HYDROLYSIS OF SUBSTOICHIOMETRIC ATP BY THE F1-ATPASE FROM ESCHERICHIA-COLI Reviewed

    E MUNEYUKI, M YOSHIDA, DA BULLOUGH, WS ALLISON

    BIOCHIMICA ET BIOPHYSICA ACTA   1058 ( 2 )   304 - 311   1991.6

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  • AROMATIC RINGS OF TYROSINE RESIDUES AT ADENINE-NUCLEOTIDE BINDING-SITES OF THE BETA-SUBUNITS OF F1-ATPASE ARE NOT NECESSARY FOR ATPASE ACTIVITY Reviewed

    M ODAKA, H KOBAYASHI, E MUNEYUKI, M YOSHIDA

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   168 ( 1 )   372 - 378   1990.4

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  • STEADY-STATE KINETICS OF PROTON TRANSLOCATION CATALYZED BY THERMOPHILIC F0F1-ATPASE RECONSTITUTED IN PLANAR BILAYER-MEMBRANES Reviewed

    E MUNEYUKI, Y KAGAWA, H HIRATA

    JOURNAL OF BIOLOGICAL CHEMISTRY   264 ( 11 )   6092 - 6096   1989.4

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  • KINETIC-ANALYSIS OF PROTON TRANSLOCATION CATALYZED BY F0F1 ATPASE Reviewed

    E MUNEYUKI, H HIRATA

    FEBS LETTERS   234 ( 2 )   455 - 458   1988.7

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  • RECONSTITUTION OF THE PROTON TRANSLOCATING ATPASE FROM BOVINE HEART-MITOCHONDRIA INTO PLANAR PHOSPHOLIPID-BILAYER MEMBRANES Reviewed

    E MUNEYUKI, K OHNO, Y KAGAWA, H HIRATA

    JOURNAL OF BIOCHEMISTRY   102 ( 6 )   1433 - 1440   1987.12

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  • AN ACTIN-DEPOLYMERIZING PROTEIN (DESTRIN) FROM PORCINE KIDNEY - ITS ACTION ON F-ACTIN CONTAINING OR LACKING TROPOMYOSIN Reviewed

    E NISHIDA, E MUNEYUKI, S MAEKAWA, Y OHTA, H SAKAI

    BIOCHEMISTRY   24 ( 23 )   6624 - 6630   1985

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  • PURIFICATION OF COFILIN, A 21,000 MOLECULAR-WEIGHT ACTIN-BINDING PROTEIN, FROM PORCINE KIDNEY AND IDENTIFICATION OF THE COFILIN-BINDING SITE IN THE ACTIN SEQUENCE Reviewed

    E MUNEYUKI, E NISHIDA, K SUTOH, H SAKAI

    JOURNAL OF BIOCHEMISTRY   97 ( 2 )   563 - 568   1985

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  • ACTION OF A 19K-PROTEIN FROM PORCINE BRAIN ON ACTIN POLYMERIZATION - A NEW FUNCTIONAL CLASS OF ACTIN-BINDING PROTEINS Reviewed

    E NISHIDA, S MAEKAWA, E MUNEYUKI, H SAKAI

    JOURNAL OF BIOCHEMISTRY   95 ( 2 )   387 - 398   1984

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Books

  • 化学フロンティアシリーズ 23 1分子ナノバイオ計測

    鳥谷部祥一, 宗行英朗, 編者, 野地博行(第10章 分子モーターの1分子実験熱力学)

    化学同人  2014 

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    Responsible for pages:131-138   Language:Japanese  

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  • DOJIN BIOSCIENCE SERIES 17 一分子生物学

    上野博史, 鳥谷部祥一, 宗行英朗, 編者, 原田 慶恵, 石渡, 信一(第7章FoF1モーター)

    化学同人  2014 

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    Responsible for pages:88-101   Language:Japanese  

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  • 中央大学学術シンポジウム研究叢書 9 ナノスケール・ミクロスケールから見える ビックな世界 (新藤 斎 編著 )

    鳥谷部祥一, 宗行英朗( Role: Joint author第一章 マクスウェルの悪魔の実現|rn| ―― ミクロの世界で情報を使って熱エネルギーを自由エネルギーに変換する ――)

    中央大学出版部  2013 

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    Total pages:19   Responsible for pages:19   Language:Japanese   Book type:Scholarly book

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  • 石渡信一,桂勲,桐野豊,美宅成樹編, 生物物理学ハンドブック, 朝倉書店, 東京, 2007, xv+662p, 26.5×19cm, 本体28,000円[大学院向], ISBN978-4-254-17122-8

    宗行 英朗

    一般社団法人日本物理学会  2008.2 

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  • Handbook of ATPases, Capter 10 Rotation of F1-ATPase(Eiro Muneyuki and Masasuke Yoshida)

    Eiro Muneyuki, Masasuke Yoshida, ed. M.Futai, Y.Wada, J.Kaplan( Role: Sole authorCapter 10 Rotation of F1-ATPase)

    WILEY-VCH Verlag GmbH & Co. Weinheim  2004.8 

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    Responsible for pages:261-287   Language:English   Book type:Scholarly book

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  • 光合成辞典

    日本光合成研究会編( Role: Sole author「回転触媒説」ほか7項目)

    学会出版センター  2003.11 

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    Total pages:430   Language:Japanese   Book type:Scholarly book

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  • シリーズ・ニューバイオフィジックスⅡ-10 生物物理学とはなにか

    宗行英朗, 吉田賢右, 編者, 曽我部正博, 郷信広( Role: Sole author第1章7.エネルギー転換:ATP合成酵素)

    共立出版  2003.9 

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    Responsible for pages:88-100   Language:Japanese   Book type:Scholarly book

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  • 新ミトコンドリア学

    宗行英朗, 吉田賢右, 編者, 内海耕造, 井上正康( Role: Sole author第一章1.3エネルギー転換系と分子モーター)

    共立出版  2001.11 

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    Responsible for pages:14-21   Language:Japanese   Book type:Scholarly book

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  • シリーズ・ニューバイオフィジックスⅡ-3 ポンプとトランスポーター

    久堀徹, 宗行英朗, 野地博行, 吉田賢右, 編者, 平田肇, 茂木立志( Role: Sole author第1章1. ATP合成酵素)

    共立出版  2000.6 

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    Responsible for pages:19-40   Language:Japanese   Book type:Scholarly book

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MISC

  • Thermodynamic role of main reaction pathway and multi-body information flow in membrane transport

    Satoshi Yoshida, Yasushi Okada, Eiro Muneyuki, Sosuke Ito

    2021.12

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    The two classes of membrane transport, namely, secondary active and passive
    transport, are understood as different reaction pathways in the same protein
    structure, modeled by the 16-state model in this paper. To quantify the
    thermodynamic difference between secondary active transport and passive
    transport, we extend the second law of information thermodynamics of the
    autonomous demon in the four-state model composed of two subsystems to the
    16-state model composed of four subsystems representing the membrane transport.
    We reduce the 16 states to 4 states and derive the coarse-grained second law of
    information thermodynamics, which provides an upper bound of the free energy
    transport by the coarse-grained information flow. We also derive an upper bound
    on the free energy transport by the multi-body information flow representing
    the two-body or four-body correlations in the 16-state model by exploiting the
    cycle decomposition. The coarse-grained information flow and the multi-body
    information flows express the quantitative difference between secondary active
    and passive transport. The numerical analysis shows that the coarse-grained
    information flow is positive for secondary active transport and negative for
    passive transport. The four-body correlation is dominant in the multi-body
    information flows for secondary active transport. In contrast, the two-body
    correlation is dominant for passive transport. This result shows that both the
    sign of the coarse-grained information flow and the difference of the four-body
    correlation and the two-body correlation explain the difference of the free
    energy transport in secondary active and passive transport.

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    Other Link: http://arxiv.org/pdf/2112.04024v1

  • F1‐ATPaseのP‐loop変異体TF1(βG158A)のリン酸解離角度の解明

    成田宏夏, 星名仁志, 吉田光, 中山洋平, 鳥谷部祥一, 上野博史, 宗行英朗

    日本物理学会講演概要集(CD-ROM)   71 ( 2 )   ROMBUNNO.14pBE‐9 - 3051   2016.9

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    Language:Japanese   Publisher:一般社団法人 日本物理学会  

    &lt;p&gt;ATP合成酵素であるF1-ATPaseの反応スキームについて生成物のADPとPiの解離の関係におけるモデルが2つ考えられている。2010年に渡邊らはADPよりもPiが先に解離することを報告したが、下らはADPはPiよりも後に解離することを報告した。そこで、本研究で、私たちは変異体を用いてこの問題を議論することにした。P-loop変異体TF1(βG158A)を用いた一分子回転観察実験を行い、ATP加水分解とPi解離が遅いことを確認した。更に、ハイブリッドF1変異体を作成し、同様に回転実験から得られたdwell timeの解析を行うことで、PiはADPよりも先に解離するモデルを示唆する結果を得た。&lt;/p&gt;

    DOI: 10.11316/jpsgaiyo.71.2.0_3051

    J-GLOBAL

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  • 28pBD-6 Biological molecular motor and fluctuation

    Muneyuki Eiro

    Meeting abstracts of the Physical Society of Japan   69 ( 1 )   340 - 340   2014.3

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    Language:Japanese   Publisher:The Physical Society of Japan (JPS)  

    CiNii Books

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  • 1P163 The response of TF1 βE190D mutant to the external torque(11. Molecular motor,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Tanaka Mana, Kawakami Tomohiro, Toyabe Shoichi, Ueno Hiroshi, Kudo Seishi, Muneyuki Eiro

    Seibutsu Butsuri   54 ( 1 )   S168   2014

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.54.S168_1

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  • 3P152 Nucleotide binding to TF1 β subunit in relation to the effect of Pi(Molecular motor,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Riku Nagano, Kiyoshi Obara, Hiroshi Ueno, Eiro Muneyuki

    Seibutsu Butsuri   54 ( 1 )   S274   2014

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.54.S274_2

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  • 1P161 Observation of the rotation of F1-ATPase under the constant external torque(11. Molecular motor,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Kikuchi Yohsuke, Ueno Hiroshi, Nakayama Takahiro, Muneyuki Eiro, Toyabe Shouichi

    Seibutsu Butsuri   54 ( 1 )   S167   2014

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.54.S167_5

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  • 1P160 Inhibitory effect of Pi on F_1-ATPase P-loop mutant TF_1(βG158A)(11. Molecular motor,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

    Hoshina Hitoshi, Yoshida Hikaru, Ito Ayumi, Ito Jotaro, Toyabe Shoichi, Ueno Hiroshi, Muneyuki Eiro

    Seibutsu Butsuri   54 ( 1 )   S167   2014

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.54.S167_4

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  • 1P174 Direct observation of the rotation of V_1-ATPase from Enterococcus hirae and its torque(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

    Ueno Hiroshi, Minagawa Yoshihiro, Yamato Ichiro, Murata Takeshi, Iino Ryota, Muneyki Eiro

    Seibutsu Butsuri   53 ( 1 )   S134   2013

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    DOI: 10.2142/biophys.53.S134_5

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  • 1P175 The relationship between F_1-ATPase P-loop mutants and Pi release(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

    Yoshida Hikaru, Ito Ayumi, Ito Jotaro, Masaike Tomoko, Nishizaka Takayuki, Toyabe Shoichi, Ueno Hiroshi, Muneyuki Eiro

    Seibutsu Butsuri   53 ( 1 )   S134   2013

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    DOI: 10.2142/biophys.53.S134_6

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  • Experimental thermodynamics of single molecular motor.

    Shoichi Toyabe, Eiro Muneyuki

    Biophysics (Nagoya-shi, Japan)   9   91 - 8   2013

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    Molecular motor is a nano-sized chemical engine that converts chemical free energy to mechanical motions. Hence, the energetics is as important as kinetics in order to understand its operation principle. We review experiments to evaluate the thermodynamic properties of a rotational F1-ATPase motor (F1-motor) at a single-molecule level. We show that the F1-motor achieves 100% thermo dynamic efficiency at the stalled state. Furthermore, the motor reduces the internal irreversible heat inside the motor to almost zero and achieves a highly-efficient free energy transduction close to 100% during rotations far from quasistatic process. We discuss the mechanism of how the F1-motor achieves such a high efficiency, which highlights the remarkable property of the nano-sized engine F1-motor.

    DOI: 10.2142/biophysics.9.91

    PubMed

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  • Information-to-free-energy conversion: Utilizing thermal fluctuations.

    Shoichi Toyabe, Eiro Muneyuki

    Biophysics (Nagoya-shi, Japan)   9   107 - 12   2013

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    Maxwell's demon is a hypothetical creature that can convert information to free energy. A debate that has lasted for more than 100 years has revealed that the demon's operation does not contradict the laws of thermodynamics; hence, the demon can be realized physically. We briefly review the first experimental demonstration of Maxwell's demon of Szilard's engine type that converts information to free energy. We pump heat from an isothermal environment by using the information about the thermal fluctuations of a Brownian particle and increase the particle's free energy.

    DOI: 10.2142/biophysics.9.107

    PubMed

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  • 1P177 Comparison of the nucleotide binding to the isolated βsubunit and the F1-ATPase using the Sopped-Flow method(11.Molecular motor,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

    Nagano Riku, Obara Kiyoshi, Masaike Tomoko, Ueno Hiroshi, Muneyuki Eiro

    Seibutsu Butsuri   53 ( 1 )   S135   2013

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    DOI: 10.2142/biophys.53.S135_2

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  • 熱ゆらぎを利用する―情報熱機関の実現―

    鳥谷部 祥一, 宗行 英朗

    生物物理   52 ( 3 )   136 - 139   2012.5

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  • 27aPS-27 Recognition Mechanism of Presequence by Mitochondrial Outer Membrane Protein Tom20 using Replica-exchange Molecular Dynamics Simulation

    Komuro Yasuaki, Mori Takaharu, Miyashita Naoyuki, Muneyuki Eiro, Saitoh Takashi, Kohda Daisuke, Sugita Yuji

    Meeting abstracts of the Physical Society of Japan   67 ( 1 )   429 - 429   2012.3

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  • 1PS024 Inhibitory effect of Pi on F_1-ATPase P-loop mutant(The 50th Annual Meeting of the Biophysical Society of Japan)

    Yoshida Hikaru, Ito Ayumi, Ito Jotaro, Toyabe Syoichi, Ueno Hiroshi, Muneyuki Eiro

    Seibutsu Butsuri   52 ( 0 )   S78   2012

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    DOI: 10.2142/biophys.52.S78_2

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  • 2SI-02 Single molecule energetics of F_1-ATPase motor(2SI The art of energetic and functional efficiency in biomoiecular machines on the single molecule level,Symposium,The 50th Annual Meeting of the Biophysical Society of Japan)

    Toyabe Shoichi, Watanabe-Nakayama Takahiro, Ueno Hiroshi, Okamoto Tetsuaki, Taketani Hiroshi, Kudo Seishi, Muneyuki Eiro

    Seibutsu Butsuri   52 ( 0 )   S17   2012

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    DOI: 10.2142/biophys.52.S17_3

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  • 1PS032 Simultaneous observation of fluorescent-nucleotides and rotation of rotary molecular motor on plasmonic metal nanostructures(The 50th Annual Meeting of the Biophysical Society of Japan)

    Ueno Hiroshi, Toyabe Shoichi, Muneyuki Eiro

    Seibutsu Butsuri   52 ( 0 )   S79   2012

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    DOI: 10.2142/biophys.52.S79_4

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  • 1L1348 Simultaneous observation of fluorescent-nucleotides and rotation of F1-ATPase using plasmonic gold nanoparticle(Molecular motor 1,The 49th Annual Meeting of the Biophysical Society of Japan)

    Ueno Hiroshi, Toyabe Shoichi, Muneyuki Eiro

    Seibutsu Butsuri   51 ( 0 )   S60   2011

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    DOI: 10.2142/biophys.51.S60_4

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  • 金微粒子によるプラズモン増強電場を利用した蛍光ATPとF1‐ATPaseの回転の同時観察

    上野博史, 鳥谷部祥一, 宗行英朗

    日本生体エネルギー研究会討論会講演要旨集   37th   56 - 57   2011

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    J-GLOBAL

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  • 3A1422 pH dependency of photoelectric current of bacteriorhodopsin absorbed on Lumirror membrane(3A Biol & Artifi memb 4: Transport, Signal transduction,The 49th Annual Meeting of the Biophysical Society of Japan)

    Miyazaki Shiduma, Toyabe Shoichi, Ueno Hiroshi, Muneyuki Eiro

    Seibutsu Butsuri   51 ( 0 )   S107   2011

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    DOI: 10.2142/biophys.51.S107_1

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  • 1L1548 P05 1YE1000 Single-molecule nonequilibrium thermodynamics of molecular motor(Molecular motor 1,Early Research in Biophysics Award Candidate Presentations,Early Research in Biophysics Award,The 49th Annual Meeting of the Biophysical Society of Japan

    Toyabe Shoichi, Ueno Hiroshi, Muneyuki Eiro

    Seibutsu Butsuri   51 ( 0 )   S62   2011

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    DOI: 10.2142/biophys.51.S62_2

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  • 26aTF-9 Estimating potential profile of molecular motor from single-molecule trajectory

    Toyabe Shoichi, Ueno Hiroshi, Muneyuki Eiro

    Meeting Abstracts of the Physical Society of Japan   66 ( 0 )   308 - 308   2011

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  • Single-molecule nonequilibrium energetics of a molecular-motor F1-ATPase

    物性研究   95 ( 3 )   320 - 322   2010.12

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    Other Link: http://dl.ndl.go.jp/info:ndljp/pid/10942999

  • 23aTF-3 Converting information to energy by feedback control

    Toyabe Shoichi, Sagawa Takahiro, Ueda Masahito, Muneyuki Eiro, Sano Masaki

    Meeting abstracts of the Physical Society of Japan   65 ( 2 )   212 - 212   2010.8

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  • Nonequilibrium Energetics of a Single F-1-ATPase Molecule

    Shoichi Toyabe, Takahiro Watanabe-Nakayama, Tetsuaki Okamoto, Seishi Kudo, Eiro Muneyuki

    BIOPHYSICAL JOURNAL   98 ( 3 )   167A - 168A   2010.1

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  • 高時間・空間分解能を持つシンプルな暗視野顕微鏡の蛍光同時観察への応用

    上野博史, 飯野亮太, 田端和仁, 榊原昇一, 宗行英朗, 野地博行

    日本生体エネルギー研究会討論会講演要旨集   36th   2010

  • 3P169 Thermodynamic parameters of nucleotide binding to the catalytic sites of Fl-ATPase revealed by fluorescence measurement.(Molecular motor,The 48th Annual Meeting of the Biophysical Society of Japan)

    Kikuchi Yosuke, Naka Yusuke, Osakabe Hidemitsu, Toyabe Shoichi, Ueno Hiroshi, Muneyuki Eiro

    Seibutsu Butsuri   50 ( 2 )   S174   2010

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    DOI: 10.2142/biophys.50.S174_5

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  • レーザ光による金表面でのナノ物質操作=DNAの伸張固定およびナノ微粒子の収集を中心に=

    藤井翔, 芳賀正明, 宗行英朗

    光アライアンス   21 ( 12 )   19 - 23   2010

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  • 27pQE-6 Stall torque of F_1-ATPase

    Toyabe Shoichi, Watanabe-Nakayama Takahiro, Okamoto Tetsuaki, Kudo Seishi, Muneyuki Eiro

    Meeting Abstracts of the Physical Society of Japan   64 ( 0 )   287 - 287   2009

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  • 23pYD-11 Molecular dynamics study of ε subunit of F_1-ATPase

    Muneyuki Eiro, Tsuji Yosuke, Sugita Yuji

    Meeting abstracts of the Physical Society of Japan   63 ( 1 )   359 - 359   2008.2

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  • 外部トルク存在下でのF1モーターの回転(Rotation of F1-motor in the presence of external torque)

    中山 隆宏, 渡部, 吉田 賢右, 杉山 滋, 工藤 成史, 宗行 英朗

    生物物理   47 ( Suppl.1 )   S242 - S242   2007.11

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  • F1-ATPase rotates by an asymmetric, sequential mechanism using all three catalytic subunits(vol 14, pg 841, 2007)

    Takayuki Ariga, Eiro Muneyuki, Masasuke Yoshida

    NATURE STRUCTURAL & MOLECULAR BIOLOGY   14 ( 10 )   984 - 984   2007.10

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  • 18pTC-9 A model of autonomous free energy transducer based on coupled ratchet subsystems.

    Muneyuki Eiro, Sekimoto Ken

    Meeting abstracts of the Physical Society of Japan   62 ( 1 )   331 - 331   2007.2

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  • 3P158 Rotation of F_1-motor in the presence of external torque(Molecular motors,Poster Presentations)

    Nakayama-Watanabe Takahiro, Yoshida Masasuke, Sugiyama Shigeru, Kudo Seishi, Muneyuki Eiro

    Seibutsu Butsuri   47 ( 0 )   S242   2007

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    DOI: 10.2142/biophys.47.S242_3

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  • 生体膜における水素イオン濃度勾配の利用

    宗行 英朗

    化学と教育   55 ( 12 )   610 - 613   2007

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    DOI: 10.20665/kakyoshi.55.12_610

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  • 26pYW-7 Direct observation of rotation with interim state in Fi-ATPase

    Tsumuraya M., Oiwa K., Muneyuki E., Nishizaka T.

    Meeting abstracts of the Physical Society of Japan   60 ( 1 )   367 - 367   2005.3

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  • 2P176 Application of external force with traveling wave electric field to F_1 motor

    Watanabe-Nakayama T, Muneyuki E, Sugiyama S, Kudo S, Yoshida M

    Seibutsu Butsuri   45 ( 0 )   S163   2005

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    DOI: 10.2142/biophys.45.S163_4

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  • 14aPS-85 Site-Occupancy and ATPase Activity of F_1 ATPase (ΔNC mutant)

    Kon Rieko, Muneyuki Eiro, Sakaki Naoyoshi, Adachi Kengo, Yoshida Masasuke, Kinosita Kazuhiko Jr

    Meeting abstracts of the Physical Society of Japan   59 ( 2 )   258 - 258   2004.8

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  • "Chemomechanical coupling in F1-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation"

    Takayuki Nishizaka, Kazuhiro Oiwa, Hiroyuki Noji, Shigeki Kimura, Eiro Muneyuki, Masasuke Yoshida, Kazuhiko Kinosita, J

    Nature Structural & Molecular biology   11 ( 2 )   142 - 148   2004

  • Analysis of the two catalytic events that induce a 30 degrees substep rotation of F-1-ATPase

    K Shimabukuro, E Muneyuki, M Yoshida

    BIOPHYSICAL JOURNAL   84 ( 2 )   456A - 457A   2003.2

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  • 2O1345 F_1-ATPase Changes its COnformations upon Phosphate Release

    Masaike T, Muneyuki E, Noji H, Kinosita K, Yoshida M

    Seibutsu Butsuri   42 ( 2 )   S144   2002

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    DOI: 10.2142/biophys.42.S144_4

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  • F1-ATPase変異体α3β(E190D)3γの回転解析

    島袋勝弥, 宗行英朗, 吉田賢右

    日本生物物理学会年会講演予稿集   40th   2002

  • Effect of free energy of ATP hydrolysis on the rotation of rsubunit in F1-ATPase

    Noji H., Yasuda R., Muneyuki E., Yoshida M., Kinoshita K. Jr

    Biophysics   38 ( 2 )   S43   1998.9

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  • Transport properties of halorhodopsin

    Muneyuki E., Okuno D., Asaumi M., Yoshida M., Mogi T.

    Biophysics   38 ( 2 )   S126   1998.9

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  • バクテリオロドプシンによる光プロトンポンプの輸送特性とイオンポンプの確率的モデル-イオンポンプとイオンチャンネルの接点-

    宗行英朗

    膜   22 ( 6 )   322 - 330   1997.6

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  • Delta mu H+ dependency of proton translocation by bacteriorhodopsin and a stochastic energization-relaxation channel model (vol 100, pg 19688, 1996)

    E Muneyuki, M Ikematsu, M Yoshida

    JOURNAL OF PHYSICAL CHEMISTRY B   101 ( 5 )   846 - 846   1997.1

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  • 地上最小のモーター,ATP合成酵素 Invited

    吉田 賢右, 野地 博行, 宗行 英朗

    蛋白質 核酸 酵素   42 ( 9 )   1396 - 1406   1997

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  • 地上最小のモーター,ATP合成酵素 Invited

    吉田 賢右, 野地 博行, 宗行 英朗

    蛋白質 核酸 酵素   42 ( 9 )   1396 - 1406   1997

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  • 触媒部位の微小変化とサブユニットの全体変化

    天野 豊己, 柳沢 諭, 野地 博行, 宗行 英朗, 久堀 徹, 吉田 賢右

    日本分子生物学会年会プログラム・講演要旨集   19   266 - 266   1996.8

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  • バクテリオロドプシンによるプロトン輸送の△pH及び△Ψ依存性

    宗行 英朗, 吉田 賢右

    日本分子生物学会年会プログラム・講演要旨集   19   271 - 271   1996.8

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  • F_1-ATPase分子内でのβサブユニット同士の接触

    野地 博行, 天野 豊己, 宗行 英朗, 久堀 徹, 吉田 賢右

    日本分子生物学会年会プログラム・講演要旨集   19   267 - 267   1996.8

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  • 好熱菌 F1-ATPaseにおけるヌクレオチドの結合と加水分解活性の解析

    平尾 潤, 宗行 英朗, 吉田 賢右

    日本分子生物学会年会プログラム・講演要旨集   19   266 - 266   1996.8

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  • 平面リン脂質二重層膜を使ったイオンチャンネルの測定

    浜本敏郎, 宗行英朗, 平田肇

    細胞工学   7 ( 1 )   69 - 78   1988.1

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  • 平面リン脂質二重層膜を使ったイオンチャネルの測定

    浜本 敏郎, 宗行 英朗, 平田 肇

    細胞工学   7 ( 1 )   69 - 78   1988

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Presentations

  • F1-ATPaseの回転機構に関する分子動力学解析

    本橋昌大, 大出真央, 小林千草, Jaewoon Jung, 宗行英朗, 杉田有治

    SCBPJ最終報告会  2025.1 

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  • ライオ電顕データ駆動によるATPase動作過程の状態遷移サンプリング

    本橋昌大, 大出真央, 小林千草, Jaewoon Jung, 宗行英朗, 杉田有治

    第11回HPCI成果報告会  2024.10 

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  • 全原子分子動力学による回転分子モーターF1-ATPaseの自発的回転機構解明”

    本橋昌大, 大出真央, 小林千草, Jaewoon Jung, 宗行英朗, 杉田有治

    第13回分子モーター討論会  2024.9 

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  • Spontaneous γ subunit rotation upon conformational changes of the α β subunits in F1-ATPase

    Masahiro Motohashi, Mao Oide, Chigusa Kobayashi, Jaewoon Jung, Eiro Muneyuki, Yuji Sugita

    ACS Fall 2024  2024.8 

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  • Spontaneous γ subunit rotation upon conformational changes of the α β subunits in F1-ATPase

    Masahiro Motohashi, Mao Oide, Chigusa Kobayashi, Jaewoon Jung, Eiro Muneyuki, Yuji Sugita

    IUPAB2024  2024.6 

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  • Conformational change mechanisms driving the rotation of F1-ATPase

    Masahiro Motohashi, Mao Oide, Chigusa Kobayashi, Jaewoon Jung, Eiro Muneyuki, Yuji Sugita, ”

    BIE2024  2024.2 

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  • Conformational change mechanisms driving the rotation of F1-ATPase

    2023.11 

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  • Conformational change mechanisms driving the rotation of F1-ATPase

    Masahiro Motohashi, Mao Oide, Chigusa Kobayashi, Jaewoon Jung, Eiro Muneyuki, Yuji Sugita

    CCP2023  2023.8 

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  • F1-ATPaseのエネルギー変換効率と熱安定性

    岡庭有明, 田中真奈, 中山洋平, 宗行英朗

    日本物理学会 第73回年次大会  2018.9 

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  • F1-ATPaseにゆらぎの熱力学を適用する上での困難

    飛田直通, 中山洋平, 宗行英朗

    日本物理学会 第73回年次大会  2018.9 

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  • On the timing of phosphate release in the ATPase reaction by TF1

    Eiro Muneyuki, Yohei Nakayama, Shoichi Toyabe, Hiroshi Ueno

    日本生物物理学会第56回年会  2018.9 

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  • Thermodynamic Efficiency of F1-ATPase at High Temperature

    Tomoaki Okaniwa, Yohei Nakayama, Eiro Muneyuki

    日本生物物理学会第56回年会  2018.9 

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  • F1-ATPaseのエネルギー変換効率と熱安定性

    岡庭 有明, 田中 真奈, 中山 洋平, 宗行 英朗

    日本物理学会講演概要集  2018  一般社団法人 日本物理学会

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  • F1-ATPaseにゆらぎの熱力学を適用する上での困難

    飛田 直通, 中山 洋平, 宗行 英朗

    日本物理学会講演概要集  2018  一般社団法人 日本物理学会

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  • 回転するF1モーターからの熱の散逸

    飛田直通, 中山洋平, 宗行英朗

    生体運動研究合同班会議  2018.1 

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  • F1-ATPaseの一分子回転観察に基づくATP加水分解化学ポテンシャル差の測定

    飛田 直通, 宗行 英朗, 中山 洋平

    日本物理学会講演概要集  2017  一般社団法人 日本物理学会

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    <p>ATPの加水分解によって駆動される回転分子モーターF1-ATPaseのエネルギー論を調べた。特に駆動力である化学ポテンシャルの大きさを一分子実験によって決定するために、回転1自由度の有効ポテンシャルによる記述に基づき、回転の角度の時系列に局所詳細釣り合いを当てはめた。その結果、360°回転で解放される化学自由エネルギーの大きさがATP3分子分に相当することが明らかになった。</p>

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  • 12-6-4 Lennard-Jones力場を用いた金属蛋白質の分子動力学計算

    湯浅 拓也, 八木 清, 宗行 英朗, 杉田 有治

    日本物理学会講演概要集  2017  一般社団法人 日本物理学会

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    <p>従来の古典力場では,r^-12^項とr^-6^項からなる12-6 Lennard-Jones (LJ)モデルが用いられてきた.しかし,このモデルは,電荷-誘起双極子,双極子-誘起双極子間相互作用を考慮していないため,金属蛋白質における金属イオンの配位構造を正確に表現できない.最近,この欠点を克服するため,12-6 LJモデルにr^-4^項を加えた12-6-4 LJモデルが提案された.本研究では,12-6-4 LJモデルと12-6 LJモデルを用いて,金属蛋白質の構造と結合自由エネルギーを計算し,両モデルを比較する.</p>

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  • F1-ATPaseのP-loop変異体βG158Aにおけるリン酸解離の機構

    成田宏夏, 星名仁志, 吉田光, 中山洋平, 鳥谷部祥一, 上野博史, 宗行英朗, 中大理工, 東北大工, 東大工

    生体運動研究合同班会議  2017.1 

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  • The kinetics of Pi release in F1-ATPase investigated with P-loop mutations

    Hiroka Narita, Hitoshi Hoshina, Hikaru Yoshida, Yohei Nakayama, Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki

    日本生物物理学会第54回年会  2016.11 

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  • F1-ATPaseのP-loop変異体TF1(βG158A)のリン酸解離角度の解明

    成田宏夏, 星名仁志, 吉田光, 中山洋平, 鳥谷部祥一, 上野博史, 行英, 中大, 理工, 東北大, 応物

    生体運動研究合同班会議  2016.1  一般社団法人 日本物理学会

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    <p>ATP合成酵素であるF1-ATPaseの反応スキームについて生成物のADPとPiの解離の関係におけるモデルが2つ考えられている。2010年に渡邊らはADPよりもPiが先に解離することを報告したが、下らはADPはPiよりも後に解離することを報告した。そこで、本研究で、私たちは変異体を用いてこの問題を議論することにした。P-loop変異体TF1(βG158A)を用いた一分子回転観察実験を行い、ATP加水分解とPi解離が遅いことを確認した。更に、ハイブリッドF1変異体を作成し、同様に回転実験から得られたdwell timeの解析を行うことで、PiはADPよりも先に解離するモデルを示唆する結果を得た。</p>

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  • F1-ATPaseにおける隠れたエントロピー生成

    中山 洋平, 田中 真奈, 宗行 英朗

    日本物理学会講演概要集  2016  一般社団法人 日本物理学会

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    <p>回転分子モーターF_1-ATPaseは、回転角度の他に化学状態の自由度を持つが、通常の一分子実験では回転角度のみの観察しか行えない隠れた変数を持つ系であり、そのために熱ゆらぎの顕著な系におけるエネルギー収支を評価する枠組みであるゆらぎのエネルギー論は素朴には適用することができない。本講演では、F_1-ATPaseの回転の駆動力であるヌクレオチドの化学ポテンシャル差を回転角度の時系列のみから求める方法について議論する。</p>

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  • F1-ATPaseのP-loop変異体TF1(βG158A)のリン酸解離角度の解明

    成田宏夏, 星名仁志, 吉田光, 中山洋平, 鳥谷部祥一, 上野博史, 行英, 中大, 理工, 東北大, 応物

    生体運動研究合同班会議  2016.1 

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  • 好熱菌F1のβE190残基は構造を安定化することにより,F1の高効率エネルギー変換に貢献する

    田中真奈, 河上知広, 中山洋平, 鳥谷部祥一, 上野博史, 工藤成史, 行英, 中大, 理工, 東北大, 応物

    生体運動研究合同班会議  2016.1 

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  • pH dependence of the photoinduced proton transfer in proteorhodopsin under alkaline conditions

    田母神 淳, 佐藤 慶太郎, 黒川 少名, 山田 匠, 奈良 敏文, 出村 誠, 菊川 峰志, 宗行 英朗, 加茂 直樹, 松山大・薬, 中央大, 理工, 北大, 生命科学院

    第53回日本生物物理学会年会  2015.9 

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  • The relationship between F1-ATPase P-loop mutant TF1(βG158A) and Pi release

    Hiroka Narita, Hitoshi Hoshina, Hikaru Yoshida, Ayumi Ito, Jotaro Ito, Yohei Nakayama, Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki, Dept. of, Physics. Chuo Univ, Faculty of, Physics. Tohoku Univ, School of Engineering, The University of Tokyo

    第53回日本生物物理学会年会  2015.9 

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  • Rotation of β E190D Mutant of F1-ATPase

    E. Muneyuki, M. Tanaka, T. Kawakami, H. Ueno, Y. Nakayama, S. Toyabe

    International Symposium on Fluctuation and Structure out of Equilibrium 2015  2015.8 

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  • 回転分子モーターF1-ATPaseの機能解析

    宗行 英朗

    回転分子モーターF1-ATPaseの機能解析  2015.6 

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  • F1-ATPase βE190D変異体の回転特性

    宗行 英朗, 田中 真奈, 河上 知宏, 鳥谷部 祥一, 上野 博史

    新学術領域「ゆらぎと構造」第2回公開シンポジウム  2015.5 

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  • 外力存在下のF1-ATPaseの回転観察

    菊池洋輔, 工, 鳥谷部祥一, 工学研究科, 上野博史

    2015年 生体運動研究合同班会議  2015.1 

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  • 改良した多リン酸力場を用いた ATP/ADP 結合状態の筋小胞体カルシウムポンプの分子動力学計算

    小室 靖明, 李 秀栄, 小林 千草, 宗行 英朗, 杉田 有治

    第52回日本生物物理学会年会  2014.9 

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  • F1-ATPase のP-loop変異体TF1(βG158A)に対するリン酸の阻害効果

    星名 仁志, 吉田 光, 伊藤 亜由美, 伊藤 丈太郎, 鳥谷部 祥一, 上野 博史, 宗行 英朗, 中央大, 物理, 東北大, 物理, 教職員, 東大, 院, 学

    第52回日本生物物理学会年会  2014.9 

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  • Rotor-Stator Interactions in V1 and Vo from Enterococcus hirae V-ATPase

    上野 博史, 皆川 慶嘉, 原 舞雪, 山登 一郎, 野地 博行, 村田 武志, 飯野 亮太, 宗行 英朗, 中大, 理工, 東大, 院応化, 東京理科大, 基礎工, 千葉大, 院理, 自然科学研究機構, 岡崎統合バイ

    第52回日本生物物理学会年会  2014.9 

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  • 一定外力下でのF1の回転の観察

    菊池 洋輔, 上野 博史, 中山 隆宏, 宗行 英朗, 鳥谷部 祥一, 中央大学物理学科, ミュンヘン大学物理

    第52回日本生物物理学会年会  2014.9 

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  • TF1 &beta;E190D変異体の外部トルク依存性

    田中 真奈, 河上 知広, 鳥谷部 祥一, 上野 博史, 工藤 正史, 宗行 英朗, 中央大, 物理, 東北大

    第52回日本生物物理学会年会  2014.9 

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  • 28pBD-6 生物分子モーターと揺らぎ(28pBD 領域11,領域12合同シンポジウム:量子・生命・ソフトマターにおける動的揺らぎの普遍法則の現在と未来,領域11(物性基礎論・統計力学・流体物理・応用数学・社会経済物理))

    宗行 英朗

    日本物理学会講演概要集  2014  一般社団法人 日本物理学会

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  • Single molecule study of a molecular motor: from biochemistry to physics

    Solvay Workshop on THERMODYNAMICS OF SMALL SYSTEMS  2013.12 

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  • 回転電場を用いた外力存在下でのF1-ATPaseの回転観察

    Yohsuke Kikuchi, Takahiro Nakayama, Shoichi Toyabe, Eiroh Muneyuki, Dept. Phys, Univ. Chuo, Fac. Phys, Univ. Munchen

    第51回日本生物物理学会年会  2013.10 

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  • Sopped-Flow法を用いたβサブユニット単体とF1-ATPaseへのヌクレオチド結合の比較

    Riku Nagano, Kiyoshi Obara, Tomoko Masaike, Hiroshi Ueno, Eiro Muneyuki, Dept. of, Physics. Chuo Univ, Tokyo University of science

    第51回日本生物物理学会年会  2013.10 

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  • 改良したATP分子力場を用いた筋小胞体カルシウムポンプの分子動力学計算

    Yasuaki Komuro, Chigusa Kobayashi, Suyong Re, Eiro Muneyuki, Yuji Sugita, Chuo Univ, Dept. Phys, RIKEN, RIKEN AICS, RIKEN QBiC

    第51回日本生物物理学会年会  2013.10 

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  • レプリカ交換分子動力学計算によるPA化糖鎖の立体構造解析

    Shigehisa Watabe, Suyong Re, Eiro Muneyuki, Yuji Sugita, Dept. Phys, Univ. Chuo, Riken, ASI, Riken, AICS, Riken, QBi

    第51回日本生物物理学会年会  2013.10 

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  • F1-ATPaseのP-loop変異体とリン酸解離の関係

    Hikaru Yoshida, Ayumi Ito, Jotaro Ito, Tomoko Masaike, Takayuki Nishizaka, Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki, Dept, of, Physics. Chuo Univ, School of Engineering, The university of Tokyo, Faculty of Science, Technology, Tokyo, Univercity of Science, Dept. of Phys, Univ. Gakushuin, Faculty of Physics, LMU Munich

    第51回日本生物物理学会年会  2013.10 

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  • Free Energy Analysis on the Tom20-Presequence Complex in Solution for Understanding a Dynamic-Equilibrium Model

    Komuro, Yasuaki, Miyashita, Naoyuki, Mori, Takaharu, Muneyuki, Eiro, Saitoh, Takashi, Kohda, Daisuke, Sugita, Yuji

    BIOPHYSICAL JOURNAL  2013.1 

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  • F1ATPaseβサブユニットへのヌクレオチド結合の解析

    菊池 洋輔

    2013年 生体運動研究合同班会議  2013.1 

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  • 分子モーター F1-ATPase によるエネルギー変換特性

    第26回分子シミュレーション討論会  2012.11 

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  • 筋小胞体カルシウムポンプのATP/ADP結合状態の分子動力学計算

    小室靖明, 小林千草, 宗行英朗, 杉田有治

    第26回分子シミュレーション討論会  2012.11 

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  • レプリカ交換分子動力学計算による糖鎖力場の検証

    渡部茂久, 李秀栄, 二島渉, 宗行英朗, 杉田有治

    第26回分子シミュレーション討論会  2012.11 

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  • F1-ATPase モーターの一分子エネルギー論

    Shoichi Toyabe, TakahiroWatanabe-Nakayama, Hiroshi Ueno, Tetsuaki Okamoto, Hiroshi Taketani, Seishi Kudo, Eiro Muneyuki

    第50回日本生物物理学会年会  2012.9 

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  • TF1βE190D変異体の外部トルクに対する影響

    Tomohiro Kawakami, Shoici Toyabe, Hiroshi Ueno, Seishi Kudo, Eiro Muneyuki

    第50回日本生物物理学会年会  2012.9 

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  • Effect of nucleotide structure on nucleotide binding and rotationof F1-ATPase

    Yohsuke Kikuchi, Masaike Tomoko, Shoichi Toyabe, Hiroshi Ueno, Eiroh Muneyuki

    第50回日本生物物理学会年会  2012.9 

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  • F1-ATPaseのP-loop変異体に対するリン酸の阻害効果

    Hikaru Yoshida, Ayumi Ito, Jotaro Ito, Syoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki

    第50回日本生物物理学会年会  2012.9 

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  • プラズモン増強電場を利用した蛍光ATPと回転分子モーターの回転の同時観察

    Hiroshi Ueno, Shoichi Toyabe, Eiro Muneyuki

    第50回日本生物物理学会年会  2012.9 

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  • 筋小胞体カルシウムポンプのATP結合状態の分子動力学計算

    Yasuaki Komuro, Chigusa Kobayashi, Eiro Muneyuki, Yuji Sugita

    第50回日本生物物理学会年会  2012.9 

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  • 2SI-02 F_1-ATPaseモーターの一分子エネルギー論(2SI 一分子レベルで生体分子機械のエネルギーと機能効率を考える,シンポジウム,日本生物物理学会第50回年会(2012年度))

    Toyabe Shoichi, Watanabe-Nakayama Takahiro, Ueno Hiroshi, Okamoto Tetsuaki, Taketani Hiroshi, Kudo Seishi, 宗行 英朗

    生物物理  2012  一般社団法人 日本生物物理学会

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  • 27aPS-27 ミトコンドリアへの輸送蛋白質に付加されたプレ配列認識機構のレプリカ交換分子動力学計算(27aPS 領域12ポスターセッション,領域12(ソフトマター物理,化学物理,生物物理))

    小室 靖明, 森 貴治, 宮下 尚之, 宗行 英朗, 齊藤 貴士, 神田 大輔, 杉田 有治

    日本物理学会講演概要集  2012  一般社団法人 日本物理学会

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  • F1-ATPase の回転ポテンシャル

    鳥谷部祥一, 上野博史, 宗行英朗

    2012年生体運動研究合同班会議  2012.1 

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  • ミトコンドリア外膜タンパク質Tom20-プレ配列ペプチド複合体の分子動力学計算

    小室靖明, 森貴治, 宗行英朗, 齊藤貴士, 神田大輔, 杉田有治

    第49回日本生物物理学会年会  2011.9 

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  • 分子モーターの1分子非平衡熱力学

    鳥谷部祥一, 上野博史, 宗行英朗

    第49回日本生物物理学会年会  2011.9 

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  • 表面プラズモン共鳴を引き起こす金微粒子を用いた蛍光ヌクレオチドとF1-ATPaseの回転の同時観察

    上野博史, 鳥谷部祥一, 宗行英朗

    第49回日本生物物理学会年会  2011.9 

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  • F1-ATPaseの触媒部位に対するヌクレオチド結合の温度依存性

    菊池洋輔, 中裕佑, 小酒部秀光

    第49回日本生物物理学会年会  2011.9 

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  • 低pH条件下におけるプロテオロドプシンの速いプロトン放出について

    田母神淳, 菊川峰志, 下野和実, 奈良敏文, 宗行英朗, 加茂直樹

    第49回日本生物物理学会年会  2011.9 

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  • pH dependency of photoelectric current of bacteriorhodopsin absorbed on Lumirror membrane

    宮崎師夫, 鳥谷部祥一, 上野博史, 宗行英朗

    第49回日本生物物理学会年会  2011.9 

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  • 1分子トラジェクトリから分子モーターのポテンシャルを推定する

    鳥谷部祥一, 上野博史, 宗行英朗

    日本物理学会  2011.3 

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  • 1L1548 P05 1YE1000 分子モーターの1分子非平衡熱力学(分子モーター1,日本生物物理学会若手奨励賞選考会,若手招待講演,第49回日本生物物理学会年会)

    鳥谷部 祥一, 上野 博史, 宗行 英朗

    生物物理  2011  一般社団法人 日本生物物理学会

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  • 26aTF-9 1分子トラジェクトリから分子モーターのポテンシャルを推定する(26aTF 生体分子機械・非平衡定常系,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))

    鳥谷部 祥一, 上野 博史, 宗行 英朗

    日本物理学会講演概要集  2011  一般社団法人 日本物理学会

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  • ミトコンドリア外膜タンパク質Tom20-プレ配列複合体の分子動力学計算

    Yasuaki Komuro, Takaharu Mori, Eiro Muneyuki, Takashi Saito, Daisuke Kohda, Yuji Sugita

    日本生物物理学会第48回年会  2010.9 

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  • ハイブリッド F1-ATPaseの角度解析によって見出されたもう一つのサブ-サブステップ回転

    Takayuki Ariga, Eiro Muneyuki, Masasuke Yoshida

    日本生物物理学会第48回年会  2010.9 

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  • Information-heat engine as a model system of molecular motors

    Shoichi Toyabe, Takahiro Sagawa, Masahito Ueda, Eiro Muneyuki, Masaki Sano

    日本生物物理学会第48回年会  2010.9 

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  • ヌクレオチドとF1-ATPaseとの結合の熱力学パラメータの測定

    Yosuke Kikuchi, Yusuke Naka, Hidemitsu Osakabe, Shoichi Toyabe, Hiroshi Ueno, Eiro Muneyuki

    日本生物物理学会第48回年会  2010.9 

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  • F1-ATPaseのβサブユニット(Y341W)単体へのヌクレオチド結合の温度依存性

    Yusuke Naka, Hidemitsu Osakabe, Tomoko Masaike, Tetsuaki Okamoto, Eiro Muneyuki

    日本生物物理学会第48回年会  2010.9 

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  • 2 mM ATPのDMSO存在下におけるF1-ATPaseのステップ運動

    Kazuhiro Nishizuka, Shoiti Toyabe, Tetuaki Okamoto, Eiro Muneyuki

    日本生物物理学会第48回年会  2010.9 

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  • 23aTF-3 非平衡フィードバック制御による情報-エネルギー変換(23aTF 生体分子機械,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))

    鳥谷部 祥一, 沙川 貴大, 上田 正仁, 宗行 英朗, 佐野 雅己

    日本物理学会講演概要集  2010  一般社団法人 日本物理学会

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  • F1-ATPaseのATP結合イベントと温度依存性の高いイベントの関係

    岡本哲明, 鳥谷部祥一, 宗行英朗

    生体運動研究合同班会議  2010.1 

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  • 27pQE-6 回転分子モーターF_1-ATPaseのストールトルク(生物物理,領域12,ソフトマター物理,化学物理,生物物理)

    鳥谷部 祥一, 中山 隆宏, 岡本 哲明, 工藤 正史, 宗行 英朗

    日本物理学会講演概要集  2009  一般社団法人 日本物理学会

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  • 23pYD-11 F_1-ATPase εサブユニットの分子動力学計算による研究(生物物理,領域12,ソフトマター物理,科学物理,生物物理)

    宗行 英朗, 辻 洋介, 杉田 有治

    日本物理学会講演概要集  2008  一般社団法人 日本物理学会

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  • 18pTC-9 二つのラチェットを連結した,自律的な自由エネルギー変換モデル(生物物理,領域12,ソフトマター物理,化学物理,生物物理)

    宗行 英朗, 関本 謙

    日本物理学会講演概要集  2007  一般社団法人 日本物理学会

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  • 3P241 SnO_2透明電極を用いた測定によるプロテオロドプシン(PR)の光誘起プロトン移動について(光生物(視覚・光受容),ポスター発表,第45回日本生物物理学会年会)

    田母神 淳, 菊川 峰志, 宮内 正二, 宗行 英朗, 加茂 直樹

    生物物理  2007  一般社団法人 日本生物物理学会

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  • 3P158 外部トルク存在下でのF1モーターの回転(分子モーター,ポスター発表,第45回日本生物物理学会年会)

    中山(渡部) 隆宏, 吉田 賢右, 杉山 滋, 工藤 成史, 宗行 英朗

    生物物理  2007  一般社団法人 日本生物物理学会

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  • 3P157 F_1-ATPaseのATP加水分解中のヌクレオチド結合数、非触媒部位にヌクレオチドが結合できない変異体を使って(分子モーター,ポスター発表,第45回日本生物物理学会年会)

    下-昆 理恵子, 宗行 英朗, 足立 健吾, 古池 晶, 酒井 坦, 吉田 賢右, 木下 一彦

    生物物理  2007  一般社団法人 日本生物物理学会

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  • 3P156 F_1-ATPaseは3つの部位での非対称で連続的な機構で回転する(分子モーター,口頭発表,第45回日本生物物理学会年会)

    有賀 隆行, 宗行 英朗, 吉田 賢右

    生物物理  2007  一般社団法人 日本生物物理学会

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  • 3P166 △NC変異を導入したF_1-ATPaseのATP加水分解機構(分子モーター))

    下-昆 理恵子, 宗行 英朗, 榊 直由, 足立 健吾, 古池 晶, 酒井 坦, 吉田 賢右, 木下 一彦

    生物物理  2005  一般社団法人 日本生物物理学会

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  • 2P176 F_1-ATPaseの回転に対する回転電場を用いた外力の導入(分子モーター))

    中山(渡部) 隆宏, 宗行 英朗, 杉山 滋, 工藤 成史, 吉田 賢右

    生物物理  2005  一般社団法人 日本生物物理学会

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  • 2SA05 ラチェツト的な描像とF_1-ATPaseの1分子計測とエネルギー論(ゲダンケンエクスペリメント(思考実験)のすすめ)

    宗行 英朗

    生物物理  2005  一般社団法人 日本生物物理学会

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  • 26pYW-7 1分子のF_1-ATPaseにおける中間状態を伴う回転の観察(生物物理,領域12(ソフトマター物理,化学物理,生物物理))

    圓谷 真人, 大岩 和弘, 宗行 英朗, 西坂 崇之

    日本物理学会講演概要集  2005  一般社団法人 日本物理学会

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  • 14aPS-85 ΔNC 変異を導入した F_1-ATPase のヌクレオチド結合数と ATPase 加水分解活性(ポスターセッション, 領域 11)

    昆 理恵子, 宗行 英朗, 井合 健太郎, 榊 直由, 足立 健吾, 伊藤 博康, 古池 晶, 吉田 賢右, 木下 一彦

    日本物理学会講演概要集  2004  一般社団法人 日本物理学会

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  • 3P148 F_1-ATPase変異体の1分子回転観察 : ATP-binding dwellの長さがcatalytic dwellに与える影響(分子モーター)

    島袋 勝弥, 宗行 英朗, 吉田 賢右

    生物物理  2004  一般社団法人 日本生物物理学会

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  • 3P147 △NC変異を導入したF_1-ATPaseのヌクレオチド結合数とATPase加水分解活性(分子モーター)

    下-昆 理恵子, 宗行 英朗, 榊 直由, 足立 健吾, 古池 昌, 伊藤 博康, 井合 健太郎, 吉田 賢右, 木下 一彦

    生物物理  2004  一般社団法人 日本生物物理学会

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  • 3P145 極低ATP濃度におけるF_1-ATPaseの回転とトルク(分子モーター)

    榊 直由, 下-昆 理恵子, 伊藤 博康, 足立 健吾, 宗行 英朗, 吉田 賢右, 木下 一彦, Furuike S

    生物物理  2004  一般社団法人 日本生物物理学会

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  • 極低ATP濃度におけるF_1-ATPaseの回転

    榊 直由, 昆-下 理恵子, 伊藤 博康, 足立 健吾, 古池 晶, 宗行 英朗, 吉田 賢右, 木下 一彦

    生物物理  2003  一般社団法人 日本生物物理学会

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  • F_1-ATPaseの回転軸をねじる

    井合 健太郎, 宗行 英朗, 吉田 賢右

    生物物理  2003  一般社団法人 日本生物物理学会

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  • マクロな量であるATPの加水分解自由エネルギーとF_1-ATPase-分子のステップ回転の関係

    宗行 英朗, 渡部 隆宏, 野地 博行, 西坂 崇之, 吉田 賢右

    生物物理  2003  一般社団法人 日本生物物理学会

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  • △NC変異体を用いたF1-ATPaseのヌクレオチド結合数と回転速度の測定

    昆-下 理恵子, 木下 一彦, 宗行 英朗, 井合 健太郎, 榊 直由, 足立 健吾, 伊藤 博康, 古池 晶, 牧 泰史, 吉田 賢右

    生物物理  2003  一般社団法人 日本生物物理学会

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  • ハイブリッドF_1-ATPaseを用いた協同的な回転メカニズムの1分子解析

    有賀 隆行, 島袋 勝弥, 宗行 英朗, 吉田 賢右

    生物物理  2003  一般社団法人 日本生物物理学会

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  • 2O1445 低ATP濃度におけるF_1-ATPaseの回転とヌクレオチド結合数(11.分子モーター,一般演題,日本生物物理学会第40回年会)

    榊 直由, 下 理恵子, 伊藤 博康, 足立 健吾, 宗行 英朗, 吉田 賢右, 木下 一彦

    生物物理  2002  一般社団法人 日本生物物理学会

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  • 2O1330 F_1-ATPaseの回転におけるサブステップと蛍光性ATPの同時観察(11.分子モーター,一般演題,日本生物物理学会第40回年会)

    西坂 崇之, 大岩 和弘, 木村 成輝, 木下 一彦, 吉田 賢右, 宗行 英朗, 野地 博行

    生物物理  2002  一般社団法人 日本生物物理学会

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  • 2O1345 F_1-ATPaseは結合ヌクレオチドのγリン酸解離とともに構造変化する(11.分子モーター,一般演題,日本生物物理学会第40回年会)

    政池 知子, 宗行 英朗, 野地 博行, 木下 一彦, 吉田 賢右

    生物物理  2002  一般社団法人 日本生物物理学会

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  • 2O1400 ADP inhibitionのかかりにくい変異体F1-ATPaseの酵素学的性質と回転(2)(11.分子モーター,一般演題,日本生物物理学会第40回年会)

    宗行 英朗, 鈴木 徹也, 井合 健太郎, 野地 博行, 西坂 崇之, 吉田 賢右

    生物物理  2002  一般社団法人 日本生物物理学会

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  • 2O1500 F_1-ATPase変異体αзβ(E190D)зγの回転解析(11.分子モーター,一般演題,日本生物物理学会第40回年会)

    島袋 勝弥, 宗行 英朗, 吉田 賢右

    生物物理  2002  一般社団法人 日本生物物理学会

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  • 2O1515 F1モーターの回転トルクを直接測定する(11.分子モーター,一般演題,日本生物物理学会第40回年会)

    野地 博行, 伊藤 博康, 宗行 英朗, 足立 健吾, 吉田 賢右, 木下 一彦

    生物物理  2002  一般社団法人 日本生物物理学会

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  • 3P159F1-ATPaseにおけるADP阻害の解析

    弘埜(原) 陽子, 野地 博行, 宗行 英朗, 木下 一彦, 吉田 賢右

    生物物理  2001  一般社団法人 日本生物物理学会

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  • 3P158F1-ATPaseの活性に対するMgADPの及ぼす影響

    川島 啓佑, 宗行 英朗, 木下 一彦

    生物物理  2001  一般社団法人 日本生物物理学会

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  • 1P118疎水性カチオンによるバクテリオロドプシンのプロトンポンプ活性の発現 : 三級アミン誘導体の効果

    頼光 絢子, 柴田 瑩, 植野 哲, 樋口 富彦, 宗行 英朗, 加茂 直樹

    生物物理  2001  一般社団法人 日本生物物理学会

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Research Projects

  • Understanding molecular machines from the viewpoint of information thermodynamics

    Grant number:22H04852  2022.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Chuo University

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    Grant amount: \3900000 ( Direct Cost: \3000000 、 Indirect Cost: \900000 )

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  • Understanding the energy transduction by F1 motor from the view point of information thermodynamics

    Grant number:20H05545  2020.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Chuo University

    Eiro, Muneyuki

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    Grant amount: \2860000 ( Direct Cost: \2200000 、 Indirect Cost: \660000 )

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  • F1モーターが準静的に動作できる限界を探る.

    2018.4 - 2021.3

    Chuo University  Grant-in-Aid for Scientific Research (C) 

    宗行 英朗

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \4420000 ( Direct Cost: \3400000 、 Indirect Cost: \1020000 )

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  • ATPのエネルギー総括研究

    2008.4 - 2009.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-新学術領域研究(研究領域提案型) 

    鈴木 誠

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    Grant type:Competitive

    「水を主役としたATPエネルギー変換」を解明するための領域研究が開始した。12月3日に福岡国際会議場で開催された日本生物物理学会第46回年会において学会会員に対し広くこの領域研究の趣旨を伝え、そこで対象とする課題を対象としたシンポジウムを開催した。このシンポジウムには200名を超す参加者とともにこの古くて新しい問題について討論し、この問題の重要性をアピールすることができた。広報活動の一環として、本領域の活動を紹介するホームページを立ち上げた。さっそくこの生物物理学会の年会シンポジウムにおける講演概要を掲載し、本領域がめざす目標の公示と、公募研究にもとめる内容に関する情報を随時更新する態勢で提供を始めた。12月13日には東京慈恵会医科大学において開催された「筋生理の集い」において、領域研究の紹介として総括班から3 名が発表しPRを行った。1月22日には、中央大学駿河台記念館において、第1回公開シンポジウムを開催した。ここ

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  • An experimental platform for membrane protein analysis by using micro/nano machining technologies

    Grant number:18074001  2006.4 - 2009.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-特定領域研究  Grant-in-Aid for Scientific Research on Priority Areas  The University of Tokyo

    竹内 昌治

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    Grant type:Competitive

    本研究では、膜超分子モータの機能解明の効率化を可能とする実験プラットフォームを、マイクロ・ナノ加工技術を利用して実現することを目的とする。具体的には、マイクロ・ナノ加工技術を利用して、膜形成のための小孔を作製し、再現性や安定性の向上を図る。膜構造の再構成技術は、各種の膜タンパク質の機能や特性の解明に必要不可欠なプラットフォームとである。また、本研究で注目する膜分子モータに限らず、イオンチャンネルや、薬剤排出とトランスポータなどへの応用も期待できる。これまでの国内外で発表されている人工平面膜法は、一分子の機能解析が可能で、膜電流が計測不能なトランスポータなどの活性計測にも有効であるが、脂質2重膜の再構成プロセスは運まかせで、再構成膜の再現性、安定性は極めて低いという問題があった。また、複数の脂質膜を同時に再構成するのは至難の業であった。 そこで、ここではマイクロ・ナノ加工技術を利用して、小型チ

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  • 高度好塩菌の膜インターフェースにおける物質輸送の分子論的基盤

    2005.4 - 2006.3

    文部科学省  科学研究費補助金(特定領域研究) 

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    Grant type:Competitive

    Grant amount: \2900000

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  • F1モーターに外力を加えるナノシステムの構築

    2005.4 - 2006.3

    文部科学省  科学研究費補助金(特定領域研究) 

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    Grant type:Competitive

    Grant amount: \2800000

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  • バクテリオロドプシンとカーボンナノチューブの複合型マイクロナノ光センサーの構築

    2005.4 - 2006.3

    文部科学省  科学研究費補助金(萌芽研究) 

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    Grant type:Competitive

    Grant amount: \1300000

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  • Elucidation of the input-output characteristics of F_1-motor

    Grant number:14380313  2002 - 2004

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)  Tokyo Institute of Technology

    EIRO Muneyuki

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    Grant amount: \8500000 ( Direct Cost: \8500000 )

    Biological motor proteins convert free energy of ATP hydrolysis to mechanical work, thus playing essential roles in life. But the fundamental question how they work at different free energy of ATP hydrolysis has been unanswered. This question becomes serious when we ask how stepping motion of a single motor molecule in a medium reflects the concentrations of ATP. ADP and inorganic phosphate in their individual actions. The F_1 portion of ATP synthase, also called as F_1-ATPase, is a rotary molecular motor in which central γ subunit rotates against α_3β_3 cylinder hydrolyzing ATP. Its structure is highly stable. The rotary action of this motor is processive and generates high torque. These features are ideal to explore the relationship between input free energy and output mechanical work except that this motor protein is severely inhibited by ADP. Here we overcame this problem by introducing several mutations while keeping high enzymatic activity. With a probe of beads attached, stepping rotation against viscous load was examined at a wide range of free energy by changing ADP concentration. The results showed that the work of individual step motion was not affected by the free energy of ATP hydrolysis but their frequency depended on the free energy of ATP showing a property of a molecular machine operating in an environment dominated by Brownian motion.
    In addition, we have analyzed rotations of a mutant F_1 subcomplex (E190D) which hydrolyzes ATP slowly and rotations of the wild-type F_1 employing a slowly hydrolysable substrate ATPγS (adenosine-5'-(γ-thio)-triphosphate). In both cases, interim dwells at 80° position were extended. We concluded that that cleavage of ATP takes place during the interim dwell.
    Furthermore, we could observe rotation under an optical microscope, while watching which of the three sites bound and released a fluorescent ATP analog. If we assume that the analog mimics authentic ATP, the following scheme emerges : (i)in the ATP-waiting state, one site, dictated by the orientation of γ, is empty whereas the other two bind a nucleotide ; (ii)ATP binding to the empty site drives 〜80° rotation of γ ; (iii)this triggers a reaction(s), hydrolysis and/or phosphate release but not ADP release, in the site that bound ATP one step ago ; (iv)completion of that reaction induces further 〜40° rotation.
    Thus, we could elucidate important features of the energetics and kinetics of the F1-motor.

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  • Elucidation of the Cl-transport mechanism of halorthodopsin by time-resolved electric and spectroscopic measurements

    Grant number:11680653  1999 - 2001

    Tokyo Institute of Technology  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)  Tokyo Institute of Technology

    宗行 英朗

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \3700000 ( Direct Cost: \3700000 )

    First of all, we improved the photovoltage measurements system so that it enabled us to examine the photovoltage generation from 1 μsec to 500 msec.
    Then, using the time-resolved photovoltage measurement system, we examined photovoltage kinetics of halorthodopsin from Halobacterium salinarum. As a function of chloride concentrations. The photovoltage consisted of two major components ; one with a sub-ms range time constant and the other with ms range time constant with different amplitudes., These components exhibited different Cl^- concentration dependencies (0.1 M to 9 M). We found that the time constant for the fast component was relatively independent of the Cl^- concentration, whereas the time constant for the slow component increased sigmoidally at higher Cl^- concentrations. The fast and the slow processes were attributed to charge (Cl^-) movements within the protein and related to Cl^- ejection, respectively. The laser photolysis studies of shR-membrane suspensions revealed that they correspond to the formation and the decay of the N intermediate. The photovoltage amplitude of the slow component exhibited a distorted bell-shaped Cl^- concentration dependence and the Cl^- concentration dependence of its time constant suggested a weak and highly cooperative Cl^- binding site(s) at cytoplasmic side (apparent K_D of about 5 M and Hill coefficient ≧ 5). The Cl^- concentration dependence of the photovoltage amplitude and the time constant for the slow process suggested a competition between spontaneous relaxation and ion translocation. The time constant for the relaxation was estimated to be > 100 ms.
    Electrogenic activity of the halorhodopsin (hR) from shark strain was also examined. After absorbing hR-overproduced membrane fraction onto a thin polymer film, we measured photoelectric current upon illumination at various Cl^- concentrations. The amplitude of the photoelectric current exhibited a bell-shaped Cl^- concentration dependency. It was found that R108Q mutant also exhibited photoelectric current at a high Cl^- concentration. The result indicates that R108 is important for uptaking Cl^- from the medium, but once Cl^- is located near the Schiff base, hR can exhibit electrogenicity even without R108.

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  • Elucidation of the mechanism of ion transport by bacteriorhodopsin and halorhodopsin by electrical measuements

    Grant number:09833001  1997 - 1998

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)  TOKYO INSTITUTE OF TECHNOLOGY

    MUNEYUKI Eiro

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    Grant amount: \2700000 ( Direct Cost: \2700000 )

    In the first year, I constructed an experimental system which enabled to measure the fast photoelectric response of the membrane fractions containing bacteriorhodopsin (bR) or halorhodopsin (hR) by adsorbing them onto a thin polymer film, In the second year, I examined the photocurrent of bR and its mutant, two kinds of hRs from different halophytic bacteria as a function of pH or Cl-concentrations. The result suggested that the pH dependency of the photoelectric current by bR reflected the affinities of the entrance and exit of the proton pathway of bR for protons. similar results were obtained for hRs and it was suggested that the way of ion translocation in the pump proteins were similar to th at of a multiion channel. By using a pulsed laser flash, I could measure the charge movement within the pump protein in a single turnover. The charge movement in bR occurred in the musec and msec time region whereas the charge movement in two hRs occurred in the msec and sub-msec time region. By comparing the data obtained in the electrical measurements with the data obtained in the photochemical cycle, it was suggested that the charge movement occurs during the formation and decay of the N intermediate.
    On the other hand, I carried out a theoretical model study based on the properties of proton translocation by bR The model assumes three ion binding sites and the affinity change induce the uni-directional ion translocation. Furthermore, the distribution of the high affinity state and low affinity state must deviate from equilibrium distribution in order to induce active transport. I propose this is the critical difference between ion pumps and ion channels. The experimental results obtained in the second year fairly matches this idea. Thus it seems reasonable to assume that an ion pump is a multiion channel in which the affinity change for transported ion induces active transport.

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  • Molecular mechanism of Energy conversion on the biomembrane

    Grant number:06045012  1994 - 1996

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for international Scientific Research  TOKYO Inst.Technology

    YOSHIDA Masasuke, MONTAL Mauricio, MUNEYUKI Eiro, ALLISON William S

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    Grant amount: \5000000 ( Direct Cost: \5000000 )

    In order to know how many functional catalytic sites are necessary for ATPase activity of F1-ATPase from a thermophilic Bacillus PS3, a new method to isolate homogeneous prepartion of the alpha3beta3gamma complex with 1,2, or 3 incompetent catalytic sites was developed. Ten glutamic acids (Glu・Tag) were linked to C-terminus of the catalytically incompetent beta (E190Q) subunit. Glu・Tag itself did not affect ATPase activity of the complexes. Two kinds of alpha3beta3gamma complexes, one containing beta (wild-type) and the other Glu・Tag-linked beta (E190Q), were mixed, urea-denatured, dialyzed, and alpha3beta3gamma complexes were reconstituted. Each of the complexes containing different number of Glu・Tag-linked beta (E190Q) was separated by anion-exchange chromatography and analyzed. The results were as follows. 1) Normal steady-state ATPase activity requires three intact catalytic sites. 2) Chase-acceleration, a catalytic cooperativity, requires at least two intact catalytic sites. 3) Single-site catalysis can be mediated by a single intact catalytic site alone. Re-scrambling of subunits between complexes could occur when the complex was aged under some condition and this might be one of the reasons of the previous contradictory result (Miwa et al. (1989) J.Biochem. (Tokyo) 106,730-734).

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  • 一分子観察によるF.モーターの回転と酵素反応ステップの関係の解明

    Tokyo Institute of Technology  Grant-in-Aid for Scientific Research on Priority Areas (A) 

    宗行 英朗

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \2100000 ( Direct Cost: \2100000 )

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  • 一分子観察によるF_1モーターの回転と酵素反応ステップの関係の解明

    Tokyo Institute of Technology  Grant-in-Aid for Scientific Research on Priority Areas (A) 

    宗行 英朗

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \2100000 ( Direct Cost: \2100000 )

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  • イオン輸送性ATP合成酵素のATPase活性とチャネル活性の共役

    Tokyo Institute of Technology  Grant-in-Aid for Scientific Research on Priority Areas 

    宗行 英朗

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \1800000 ( Direct Cost: \1800000 )

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  • 環状のナノ粒子集合体およびその製造方法

    芳賀 正明,小林 克彰,金井塚 勝彦,藤井 翔

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    Application no:特願2009-213578  Date applied:2009.9.15

    Announcement no:特開2011-62607  Date announced:2011.3.31

    Applicant (Organization):学校法人中央大学

  • 鎖状分子の操作方法

    芳賀 正明,小林 克彰,金井塚 勝彦,藤井 翔

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    Application no:特願2009-206143  Date applied:2009.9.7

    Announcement no:特開2011-056337  Date announced:2011.3.24

    Applicant (Organization):学校法人中央大学