Updated on 2024/07/17

写真a

 
FUJITANI Hirotsugu
 
Organization
Faculty of Science and Engineering Research Associate
Contact information
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Profile
大学院生の頃から,地球の窒素循環を駆動させる微生物(硝化菌)を対象に研究しています。培養が難しい硝化菌を獲得するための技術を開発したり,獲得した硝化菌の性状(生理学的な性質やゲノム)を調べてきました。これまでの経験から,個々の微生物が持つ増殖様式や増殖メカニズムは,私たちの予想を超えて多岐に渡っており,神秘的な生命活動が営まれていることが分かってきました。また,同種・異種微生物間相互作用によって生命活動が維持されているケースもあり,微生物といえど一人では生きられない生き物のようです。培養が困難な未培養微生物を獲得することで,科学的にも社会的にも重要かつ面白い発見が可能になります。このフィロソフィを大切にしながら,生命が持つ神秘を追求していきたいです。
External link

Degree

  • 博士(工学) ( 早稲田大学 )

  • 修士(工学) ( 早稲田大学 )

Education

  • 2013.3
     

    Waseda University   Department of Life Science and Medical Bioscience Doctor Course   doctor course   completed

  • 2009.3
     

    Waseda University   Department of Life Science and Medical Bioscience Master Course   master course   completed

  • 2007.3
     

    Waseda University   Department of Applied Chemistry   graduated

Research History

  • 2020.4 - Now

    Chuo University   理工学部   助教

  • 2019.1 - Now

    Waseda University   Research Organization for Nano and Life Innovation   Guest researcher

  • 2019.1 - 2020.3

    National Institute of Advanced Industrial  Science and Technology (AIST)   Biomedical Research Institute   Postdoctoral Researcher

  • 2018.4 - 2018.12

    Waseda University   Research Organization for Nano & Life Innovation   Assistant professor

  • 2018.1 - 2018.12

    Technical University of Denmark   Department of Environment Engineering   Guest researcher

  • 2016.4 - 2018.3

    Waseda University   Research Organization for Nano & Life Innovation   Junior researcher

  • 2013.4 - 2016.3

    Waseda University   Department of Life Science and Medical Bioscience   Research associate

▼display all

Professional Memberships

  • 環境バイオテクノロジー学会

  • International Society for Microbial Ecology

  • JAPAN SOCIETY ON WATER ENVIRONMENT

  • THE JAPANESE SOCIETY OF MICROBIAL ECOLOGY

  • 日本ゲノム微生物学会

Research Interests

  • nitrite oxidation

  • wastewater

  • cultivation

  • nitrification

  • nitrifier

  • ecophysiology

  • microbial ecology

  • environmental microbes

  • microbial interaction

  • soil

  • isolation

  • nitrogen cycle

  • genome

  • ammonia oxidation

  • heterotrophs

  • autotrophs

Research Areas

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering  / Biofunction/Bioprocess

  • Life Science / Ecology and environment

  • Life Science / Applied microbiology

  • Life Science / Biodiversity and systematics

Papers

  • Variation in growth rates between cultures hinders the cultivation of ammonia-oxidizing bacteria Reviewed

    Rino Isshiki, Hirotsugu Fujitani, Satoshi Tsuneda

    FEMS Microbiology Letters   371   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    Abstract

    Ammonia-oxidizing bacteria, key players in the nitrogen cycle, have been the focus of extensive research. Numerous novel species have been isolated and their growth dynamics were studied. Despite these efforts, controlling their growth to obtain diverse physiological findings remains a challenge. These bacteria often fail to grow, even under optimal conditions. This unpredictable growth pattern could be viewed as a survival strategy. Understanding this heterogeneous behavior could enhance our ability to culture these bacteria. In this study, the variation in the growth rate was quantified for the ammonia-oxidizing bacterium Nitrosomonas mobilis Ms1. Our findings revealed significant growth rate variation under low inoculum conditions. Interestingly, higher cell densities resulted in more stable cultures. A comparative analysis of three Nitrosomonas species showed a correlation between growth rate variation and culture failure. The greater the variation in growth rate, the higher the likelihood of culture failure.

    DOI: 10.1093/femsle/fnae013

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    Other Link: https://academic.oup.com/femsle/article-pdf/doi/10.1093/femsle/fnae013/56963691/fnae013.pdf

  • Characterisation of bacteria representing a novel Nitrosomonas clade: Physiology, genomics and distribution of missing ammonia oxidizer. Reviewed International journal

    Shuta Kikuchi, Hirotsugu Fujitani, Kento Ishii, Rino Isshiki, Yuji Sekiguchi, Satoshi Tsuneda

    Environmental Microbiology Reports   15 ( 5 )   404 - 416   2023.4

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Members of the genus Nitrosomonas are major ammonia oxidizers that catalyse the first step of nitrification in various ecosystems. To date, six subgenus-level clades have been identified. We have previously isolated novel ammonia oxidizers from an additional clade (unclassified cluster 1) of the genus Nitrosomonas. In this study, we report unique physiological and genomic properties of the strain PY1, compared with representative ammonia-oxidising bacteria (AOB). The apparent half-saturation constant for total ammonia nitrogen and maximum velocity of strain PY1 were 57.9 ± 4.8 μM NH3  + NH4 + and 18.5 ± 1.8 μmol N (mg protein)-1  h-1 , respectively. Phylogenetic analysis based on genomic information revealed that strain PY1 belongs to a novel clade of the Nitrosomonas genus. Although PY1 contained genes to withstand oxidative stress, cell growth of PY1 required catalase to scavenge hydrogen peroxide. Environmental distribution analysis revealed that the novel clade containing PY1-like sequences is predominant in oligotrophic freshwater. Taken together, the strain PY1 had a longer generation time, higher yield and required reactive oxygen species (ROS) scavengers to oxidize ammonia, compared with known AOB. These findings expand our knowledge of the ecophysiology and genomic diversity of ammonia-oxidising Nitrosomonas.

    DOI: 10.1111/1758-2229.13158

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  • Effects of Co-existing Heterotrophs on Physiology of and Nitrogen Metabolism in Autotrophic Nitrite-oxidizing <i>Candidatus</i> Nitrotoga

    Hiroto Ide, Kento Ishii, Yu Takahashi, Hirotsugu Fujitani, Satoshi Tsuneda

    Microbes and Environments   38 ( 4 )   n/a - n/a   2023

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    Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Microbial Ecology  

    DOI: 10.1264/jsme2.me23076

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  • Isolation Strategy and Characterization of Uncultured Nitrifiers Invited Reviewed

    Hirotsugu Fujitani

    Journal of Environmental Biotechnology   21 ( 1 )   53 - 58   2021.8

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

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  • Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort Reviewed

    Kanako Saito, Yuri Ota, Dieter M. Tourlousse, Satoko Matsukura, Hirotsugu Fujitani, Masamune Morita, Satoshi Tsuneda, Naohiro Noda

    Scientific Reports   11 ( 1 )   9506   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Nature Research  

    <title>Abstract</title>Droplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip “fluorescent nucleic acid probe in droplets for bacterial sorting” (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.

    DOI: 10.1038/s41598-021-88974-2

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    Other Link: http://www.nature.com/articles/s41598-021-88974-2

  • Nitrogen and oxygen isotope signatures of nitrogen compounds during anammox in the laboratory and a wastewater treatment plant Reviewed

    Shotoku Kotajima, Keisuke Koba, Daisuke Ikeda, Akihiko Terada, Kazuichi Isaka, Kazuya Nishina, Yuuya Kimura, Akiko Makabe, Midori Yano, Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda, Muneoki Yoh

    Microbes and Environments   35 ( 4 )   ME20031   2020.11

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    Isotopic fractionation factors against 15N and 18O during anammox (anaerobic ammonia oxidization by nitrite) are critical for evaluating the importance of this process in natural environments. We performed batch incubation experiments with an anammox-dominated biomass to investigate nitrogen (N) and oxygen (O) isotopic fractionation factors during anammox and also examined apparent isotope fractionation factors during anammox in an actual wastewater treatment plant. We conducted one incubation experiment with high δ18O of water to investigate the effects of water δ18O. The N isotopic fractionation factors estimated from incubation experiments and the wastewater treatment plant were similar to previous values. We also found that the N isotopic effect (15εNXR of -77.8 to -65.9‰ and 15ΔNXR of -31.3 to -30.4‰) and possibly O isotopic effect (18εNXR of -20.6‰) for anaerobic nitrite oxidation to nitrate were inverse. We applied the estimated isotopic fractionation factors to the ordinary differential equation model to clarify whether anammox induces deviations in the δ18O vs δ15N of nitrate from a linear trajectory of 1, similar to heterotrophic denitrification. Although this deviation has been attributed to nitrite oxidation, the O isotopic fractionation factor for anammox is crucial for obtaining a more detailed understanding of the mechanisms controlling this deviation. In our model, anammox induced the trajectory of the δ18O vs δ15N of nitrate during denitrification to less than one, which strongly indicates that this deviation is evidence of nitrite oxidation by anammox under denitrifying conditions.

    DOI: 10.1264/jsme2.ME20031

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  • Genomic and Physiological Characteristics of a Novel Nitrite-Oxidizing Nitrospira Strain Isolated From a Drinking Water Treatment Plant

    Hirotsugu Fujitani, Kengo Momiuchi, Kento Ishii, Manami Nomachi, Shuta Kikuchi, Norisuke Ushiki, Yuji Sekiguchi, Satoshi Tsuneda

    Frontiers in Microbiology   11   2020.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media S.A.  

    Nitrite-oxidizing bacteria (NOB) catalyze the second step of nitrification, which is an important process of the biogeochemical nitrogen cycle and is exploited extensively as a biological nitrogen removal process. Members of the genus Nitrospira are often identified as the dominant NOB in a diverse range of natural and artificial environments. Additionally, a number of studies examining the distribution, abundance, and characterization of complete ammonia oxidation (comammox) Nitrospira support the ecological importance of the genus Nitrospira. However, niche differentiation between nitrite-oxidizing Nitrospira and comammox Nitrospira remains unknown due to a lack of pure cultures. In this study, we report the isolation, physiology, and genome of a novel nitrite-oxidizing Nitrospira strain isolated from a fixed-bed column at a drinking water treatment plant. Continuous feeding of ammonia led to the enrichment of Nitrospira-like cells, as well as members of ammonia-oxidizing genus Nitrosomonas. Subsequently, a microcolony sorting technique was used to isolate a novel nitrite-oxidizing Nitrospira strain. Sequences of strains showing the growth of microcolonies in microtiter plates were checked. Consequently, the most abundant operational taxonomic unit (OTU) exhibited high sequence similarity with Nitrospira japonica (98%) at the 16S rRNA gene level. The two other Nitrospira OTUs shared over 99% sequence similarities with N. japonica and Nitrospira sp. strain GC86. Only one strain identified as Nitrospira was successfully subcultivated and designated as Nitrospira sp. strain KM1 with high sequence similarity with N. japonica (98%). The half saturation constant for nitrite and the maximum nitrite oxidation rate of strain KM1 were orders of magnitude lower than the published data of other known Nitrospira strains
    moreover, strain KM1 was more sensitive to free ammonia compared with previously isolated Nitrospira strains. Therefore, the new Nitrospira strain appears to be better adapted to oligotrophic environments compared with other known non-marine nitrite oxidizers. The complete genome of strain KM1 was 4,509,223 bp in length and contained 4,318 predicted coding sequences. Average nucleotide identities between strain KM1 and known cultured Nitrospira genome sequences are 76.7–78.4%, suggesting at least species-level novelty of the strain in the Nitrospira lineage II. These findings broaden knowledge of the ecophysiological diversity of nitrite-oxidizing Nitrospira.

    DOI: 10.3389/fmicb.2020.545190

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  • Enrichment of comammox and nitrite-oxidizing Nitrospira from acidic soil Reviewed

    Yu Takahashi, Hirotsugu Fujitani, Yuhei Hirano, Kanako Tago, Yong Wang, Masahito Hayatsu, Satoshi Tsuneda

    Frontiers in Microbiology   11 ( 1737 )   2020.7

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    DOI: 10.3389/fmicb.2020.01737

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  • MazF endoribonucleolytic toxin conserved in Nitrospira specifically cleaves the AACU, AACG, and AAUU motifs Reviewed

    Rie Aoi, Tatsuki Miyamoto, Akiko Yokota, Yuri Ota, Hirotsugu Fujitani, Satoshi Tsuneda, Naohiro Noda

    Toxins   12 ( 5 )   287   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI  

    DOI: 10.3390/toxins12050287

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  • Physiological and genomic characterization of a new “Candidatus Nitrotoga” isolate Reviewed

    Kento Ishii, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    Environmental Microbiology   22 ( 6 )   2365 - 2382   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley-Blackwell  

    DOI: 10.1111/1462-2920.15015

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  • Transcriptome analysis of the ammonia-oxidizing bacterium Nitrosomonas mobilis Ms1 reveals division of labor between aggregates and free-living cells Reviewed

    Rino Isshiki, Hirotsugu Fujitani, Satoshi Tsuneda

    Microbes and Environments   35 ( 2 )   2020.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Microbial Ecology  

    Bacteria change their metabolic states to increase survival by forming aggregates. Ammonia-oxidizing bacteria also form aggregates in response to environmental stresses. Nitrosomonas mobilis, an ammonia-oxidizing bacterium with high stress tolerance, often forms aggregates mainly in wastewater treatment systems. Despite the high frequency of aggregate formation by N. mobilis, its relationship with survival currently remains unclear. In the present study, aggregates were formed in the late stage of culture with the accumulation of nitrite as a growth inhibitor. To clarify the significance of aggregate formation in N. mobilis Ms1, a transcriptome analysis was performed. Comparisons of the early and late stages of culture revealed that the expression of stress response genes (chaperones and proteases) increased in the early stage. Aggregate formation may lead to stress avoidance because stress response genes were not up-regulated in the late stage of culture during which aggregates formed. Furthermore, comparisons of free-living cells with aggregates in the early stage of culture showed differences in gene expression related to biosynthesis (ATP synthase and ribosomal proteins) and motility and adhesion (flagella, pilus, and chemotaxis). Biosynthesis genes for growth were up-regulated in free-living cells, while motility and adhesion genes for adaptation were up-regulated in aggregates. These results indicate that N. mobilis Ms1 cells adapt to an unfavorable environment and grow through the division of labor between aggregates and free-living cells.

    DOI: 10.1264/jsme2.ME19148

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  • Successful enrichment of low-abundant comammox Nitrospira from nitrifying granules under ammonia-limited conditions Reviewed

    Hirotsugu Fujitani†, Manami Nomachi†, Yu Takahashi†, Yoshiaki Hasebe, Masahiro Eguchi, Satoshi Tsuneda

    FEMS Microbiology Letters   367 ( 1 )   fna025   2020.2

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford Academic  

    DOI: 10.1093/femsle/fnaa025

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  • Draft genome sequence of Acidovorax sp. strain NB1 isolated from a nitrite-oxidizing enrichment culture Reviewed

    Hiroto Ide, Kento Ishii, Hirotsugu Fujitani, Satoshi Tsuneda

    Microbiology Resource Announcements   8 ( 33 )   e00547-19   2019.8

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

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  • Seabird-affected taluses are denitrification hotspots and potential N2O emitters in the High Arctic Reviewed

    Hayashi, Kentaro, Tanabe, Yukiko, Ono, Keisuke, Loonen, Maarten J. J. E., Asano, Maki, Fujitani, Hirotsugu, Tokida, Takeshi, Uchida, Masaki, Hayatsu, Masahito

    Scientific Reports   8 ( 17261 )   2018.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    In High Arctic tundra ecosystems, seabird colonies create nitrogen cycling hotspots because of bird-derived labile organic matter. However, knowledge about the nitrogen cycle in such ornithocoprophilous tundra is limited. Here, we determined denitrification potentials and in-situ nitrous oxide (N2O) emissions of surface soils on plant-covered taluses under piscivorous seabird cliffs at two sites (BL and ST) near Ny-Alesund, Svalbard, in the European High Arctic. Talus soils at both locations had very high denitrification potentials at 10 degrees C (2.62-4.88 mg N kg(-1) dry soil h(-1)), near the mean daily maximum air temperature in July in Ny-Alesund, with positive temperature responses at 20 degrees C (Q10 values, 1.6-2.3). The talus soils contained abundant denitrification genes, suggesting that they are denitrification hotspots. However, high in-situ N2O emissions, indicating the presence of both active aerobic nitrification and anaerobic denitrification, were observed only at BL (max. 16.6 mu g N m(-2) h(-1)). Rapid nitrogen turnover at BL was supported by lower carbon-to-nitrogen ratios, higher nitrate content, and higher delta N-15 values in the soils at BL compared with those at ST. These are attributed to the 30-fold larger seabird density at BL than at ST, providing the larger organic matter input.

    DOI: 10.1038/s41598-018-35669-w

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  • Genomic analysis of two phylogenetically distinct Nitrospira species reveals their genomic plasticity and functional diversity Reviewed

    Norisuke Ushiki, Hirotsugu Fujitani, Yu Shimada, Tomohiro Morohoshi, Yuji Sekiguchi, Satoshi Tsuneda

    Frontiers in Microbiology   8 ( 2637 )   2018.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media S.A.  

    The genus Nitrospira represents a dominant group of nitrite-oxidizing bacteria in natural and engineered ecosystems. This genus is phylogenetically divided into six lineages, for which vast phylogenetic and functional diversity has been revealed by recent molecular ecophysiological analyses. However, the genetic basis underlying these phenotypic differences remains largely unknown because of the lack of genome sequences representing their diversity. To gain a more comprehensive understanding of Nitrospira, we performed genomic comparisons between two Nitrospira strains (ND1 and NJ1 belonging to lineages I and II, respectively) previously isolated from activated sludge. In addition, the genomes of these strains were systematically compared with previously reported six Nitrospira genomes to reveal their similarity and presence/absence of several functional genes/operons. Comparisons of Nitrospira genomes indicated that their genomic diversity reflects phenotypic differences and versatile nitrogen metabolisms. Although most genes involved in key metabolic pathways were conserved between strains ND1 and NJ1, assimilatory nitrite reduction pathways of the two Nitrospira strains were different. In addition, the genomes of both strains contain a phylogenetically different urease locus and we confirmed their ureolytic activity. During gene annotation of strain NJ1, we found a gene cluster encoding a quorum-sensing system. From the enriched supernatant of strain NJ1, we successfully identified seven types of acyl-homoserine lactones with a range of C10-C14. In addition, the genome of strain NJ1 lacks genes relevant to flagella and the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated genes) systems, whereas most nitrifying bacteria including strain ND1 possess these genomic elements. These findings enhance our understanding of genomic plasticity and functional diversity among members of the genus Nitrospira.

    DOI: 10.3389/fmicb.2017.02637

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  • Enrichment and Physiological Characterization of a Cold-Adapted Nitrite-Oxidizing Nitrotoga sp from an Eelgrass Sediment Reviewed

    Kento Ishii, Hirotsugu Fujitani, Kentaro Soh, Tatsunori Nakagawa, Reiji Takahashi, Satoshi Tsuneda

    Applied and Environmental Microbiology   83 ( 14 )   e00549-17   2017.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC MICROBIOLOGY  

    Nitrite-oxidizing bacteria (NOB) are responsible for the second step of nitrification in natural and engineered ecosystems. The recently discovered genus Nitrotoga belongs to the Betaproteobacteria and potentially has high environmental importance. Although environmental clones affiliated with Nitrotoga are widely distributed, the limited number of cultivated Nitrotoga spp. results in a poor understanding of their ecophysiological features. In this study, we successfully enriched the nonmarine cold-adapted Nitrotoga sp. strain AM1 from coastal sand in an eelgrass zone and investigated its physiological characteristics. Multistep-enrichment approaches led to an increase in the abundance of AM1 to approximately 80% of the total bacterial population. AM1 was the only detectable NOB in the bacterial community. The 16S rRNA gene sequence of AM1 was 99.6% identical to that of "Candidatus Nitrotoga arctica," which was enriched from permafrost-affected soil. The highest nitrogen oxidation rate of AM1 was observed at 16 degrees C. The half-saturation constant (K-m) and the generation time were determined to be 25 mu M NO2- and 54 h, respectively. The nitrite oxidation rate of AM1 was stimulated at concentrations of &lt;30 mM NH4Cl but completely inhibited at 50 mM NH4Cl. AM1 can grow well under specific environmental conditions, such as low temperature and in the presence of a relatively high concentration of free ammonia. These results help improve our comprehension of the functional importance of Nitrotoga.
    IMPORTANCE Nitrite-oxidizing bacteria (NOB) are key players in the second step of nitrification, which is an important process of the nitrogen cycle. Recent studies have suggested that the organisms of the novel NOB genus Nitrotoga were widely distributed and played a functional role in natural and engineered ecosystems. However, only a few Nitrotoga enrichments have been obtained, and little is known about their ecology and physiology. In this study, we successfully enriched a Nitrotoga sp. from sand in a shallow coastal marine ecosystem and undertook a physiological characterization. The laboratory experiments showed that the Nitrotoga enrichment culture could adapt not only to low temperature but also to relatively high concentrations of free ammonia. The determination of as-yet-unknown unique characteristics of Nitrotoga contributes to the improvement of our insights into the microbiology of nitrification.

    DOI: 10.1128/AEM.00549-17

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  • Nitrite oxidation kinetics of two Nitrospira strains: The quest for competition and ecological niche differentiation Reviewed

    Norisuke Ushiki, Masaru Jinno, Hirotsugu Fujitani, Toshikazu Suenaga, Akihiko Terada, Satoshi Tsuneda

    Journal of Bioscience and Bioengineering   123 ( 5 )   581 - 589   2017.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Nitrite oxidation is an aerobic process of the nitrogen cycle in natural ecosystems, and is performed by nitrite-oxidizing bacteria (NOB). Also, nitrite oxidation is a rate-limiting step of nitrogen removal in wastewater treatment plants (WWTPs). Although Nitrospira is known as dominant NOB in WWTPs, information on their physiological properties and kinetic parameters is limited. Here, we report the kinetic parameters and inhibition of nitrite oxidation by free ammonia in pure cultures of Nitrospira sp. strain ND1 and Nitrospira japonica strain NJ1, which were previously isolated from activated sludge in a WWTP. The maximum nitrite uptake rate (V-max_NO2) and the half-saturation constant for nitrite uptake (K-m_NO2) of strains ND1 and NJ1 were 45 +/- 7 and 31 +/- 5 (mu mol NO2-/mg protein/h), and 6 +/- 1 and 10 +/- 2 (mu M NO2-), respectively. The V-max_NO2 and K-m_NO2 of two strains indicated that they adapt to low-nitrite-concentration environments like activated sludge. The half-saturation constants for oxygen uptake (K-m_O2) of the two strains were 4.0 +/- 2.5 and 2.6 +/- 1.1 (mu M O-2), respectively. The K-m_O2 values of the two strains were lower than those of other NOB, suggesting that Nitrospira in activated sludge could oxidize nitrite in the hypoxic environments often found in the interiors of biofilms and flocs. The inhibition thresholds of the two strains by free ammonia were 0.85 and 4.3 (mg-NH3 I-1), respectively. Comparing the physiological properties of the two strains, we suggest that tolerance for free ammonia determines competition and partitioning into ecological niches among Nitrospira populations. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2016.12.016

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  • Detection and Diversity of the Nitrite Oxidoreductase Alpha Subunit (nxrA) Gene of Nitrospina in Marine Sediments Reviewed

    Sundas Rani, Hyeon-Woo Koh, Sung-Keun Rhee, Hirotsugu Fujitani, Soo-Je Park

    Microbial Ecology   73 ( 1 )   111 - 122   2017.1

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Nitrite-oxidizing bacteria (NOB) are chemolithoautotrophs that catalyze the oxidation of nitrite to nitrate, which is the second step of aerobic nitrification. In marine ecosystems, Nitrospina is assumed to be a major contributor to nitrification. To date, two strains of Nitrospina have been isolated from marine environments. Despite their ecological relevance, their ecophysiology and environmental distribution are understudied owing to fastidious cultivation techniques and the lack of a sufficient functional gene marker. To estimate the abundance, diversity, and distribution of Nitrospina in various marine sediments, we used nxrA, which encodes the alpha subunit of nitrite oxidoreductase, as a functional and phylogenetic marker. We observed that Nitrospina diversity in polar sediments was significantly lower than that of non-polar samples. Moreover, nxrA-like sequences revealed an unexpected diversity of Nitrospina, with approximately 41,000 different sequences based on a 95% similarity cutoff from six marine sediments. We detected nxrA gene copy numbers of up to 3.57 x 10(4) per gram of marine sediment sample. The results of this study provide insight into the distribution and diversity of Nitrospina, which is fundamentally important for understanding their contribution to the nitrogen cycle in marine sediments.

    DOI: 10.1007/s00248-016-0897-3

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  • A rapid collection of yet unknown ammonia oxidizers in pure culture from activated sludge Reviewed

    Takuma Abe, Norisuke Ushiki, Hirotsugu Fujitani, Satoshi Tsuneda

    Water Research   108   169 - 178   2017.1

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Nitrification is an important reaction in the biological nitrogen removal process in wastewater treatment plants (WWTPs). As ammonia-oxidizing microbes are slow-growing and sensitive to environmental factors such as free ammonia, pure strains are hard to obtain, preventing our understanding of their physiological characteristics. To conquer this hurdle, we report a high-throughput isolation technique based on scattering signatures, which exploits the tendency of many ammonia-oxidizing bacteria (AOB) to form microcolonies in activated sludge. The AOB microcolonies were directly sorted from the activated sludge without long incubation and enrichment bias, and were sequentially inoculated into 96-well microtiter plates containing growth medium. Phylogenetic analysis of the pure strains isolated in this study revealed a deeply branching and unrecognized lineage and diversity within the genus Nitrosomonas, beyond our expectation. (C) 2016 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.watres.2016.10.070

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  • Ecophysiology and Comparative Genomics of Nitrosomonas mobilis Ms1 Isolated from Autotrophic Nitrifying Granules of Wastewater Treatment Bioreactor Reviewed

    Soe Myat Thandar, Norisuke Ushiki, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    Frontiers in Microbiology   7 ( 1869 )   2016.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    Ammonia-oxidizing bacteria (AOB), which oxidize ammonia to nitrite in the first step of nitrification, play an important role in biological wastewater treatment systems. Nitrosomonas mobilis is an important and dominant AOB in various wastewater treatment systems. However, the detailed physiological and genomic properties of N. mobilis have not been thoroughly investigated because of limited success isolating pure cultures. This study investigated the key physiological characteristics of N. mobilis Ms1, which was previously isolated into pure culture from the nitrifying granules of wastewater treatment bioreactor. The pure culture of N. mobilis Ms1 was cultivated in liquid mineral medium with 30 mg-NL-1 (2.14 mM) of ammonium at room temperature under dark conditions. The optimum growth of N. mobilis Ms1 occurred at 27 degrees C and pH 8, with a maximum growth rate of 0.05-0.07 h(-1), which corresponded to a generation time of 10-14 h. The half saturation constant for ammonium uptake rate and the maximum ammonium uptake rate of N. mobilis Ms1 were 30.70 +/- 0.51 mu M NH4+ and 0.01 +/- 0.002 pmol NH4+ cells(-1) h(-1), respectively. N. mobilis Ms1 had higher ammonia oxidation activity than N. europaea in this study. The oxygen uptake activity kinetics of N. mobilis Ms1 were K-m(O2) = 21.74 +/- 4.01 it mu M O-2 and V-max(O2) = 0.06 +/- 0.02 pmol O-2 cells(-1) h(-1). Ms1 grew well at ammonium and NaCl concentrations of up to 100 and 500 mM, respectively. The nitrite tolerance of N. mobilis Ms1 was extremely high (up to 300 mM) compared to AOB previously isolated from activated sludge and wastewater treatment plants. The average nucleotide identity between the genomes of N. mobilis Ms1 and other Nitrosomonas species indicated that N. mobilis Ms1 was distantly related to other Nitrosomonas species. The organization of the genes encoding protein inventory involved in ammonia oxidation and nitrifier denitrification processes were different from other Nitrosomonas species. The current study provides a needed physiological and genomic characterization of N. mobilis-like bacteria and a better understanding of their ecophysiological properties, enabling comparison of these bacteria with other AOB in wastewater treatment systems and natural ecosystems.

    DOI: 10.3389/fmicb.2016.01869

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  • Physical enrichment of uncultured Accumulibacter and Nitrospira from activated sludge by unlabeled cell sorting technique Reviewed

    Kana Irie, Hirotsugu Fujitani, Satoshi Tsuneda

    Journal of Bioscience and Bioengineering   122 ( 4 )   475 - 481   2016.10

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    It is important to understand the ecology and physiology of microbes in activated sludge of wastewater treatment plants. Recently, molecular based approaches such as 16S rRNA genes and environmental genomics have illuminated black boxes in nutrient removal process and expanded our knowledge. However, most microbes responsible for the removal of phosphate and nitrogen such as Accumulibacter and Nitrospira remain uncultured. This is because optimum methodologies to concentrate these uncultured microbes and to obtain pure cultures have not been established. Here, we report a novel approach for physical enrichment of uncultured Accumulibacter and Nitrospira from microbial communities in activated sludge by a cell sorting system. Two scattering signatures representing forward scatter and side scatter of this system allowed morphological characterization of microbial particles in activated sludge. The distribution and size of microbial particles consisting of single cells, microcolonies, and aggregates depended on the levels of scattering signatures. Next generation sequencer and principal component analysis revealed each microbial population fractionated according to the levels of scattering signatures, resulting that uncultured Accumulibacter and Nitrospira could be sorted as single cells or microcolonies. Finally, quantitative fluorescence in situ hybridization analysis determined optimum fractions to collect sufficiently these target microbes from activated sludge. Consequently, this method would be very useful as an enrichment technique prior to isolation, genomic analysis, and physiological investigation of uncultured bacteria. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2016.03.020

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  • Selective isolation of ammonia-oxidizing bacteria from autotrophic nitrifying granules by applying cell-sorting and sub-culturing of microcolonies Reviewed

    Hirotsugu Fujitani, Asami Kumagai, Norisuke Ushiki, Kengo Momiuchi, Satoshi Tsuneda

    Frontiers in Microbiology   6 ( 1159 )   2015.10

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    Nitrification is a key process in the biogeochemical nitrogen cycle and biological wastewater treatment that consists of two stepwise reactions, ammonia oxidation by ammonia-oxidizing bacteria (AOB) or archaea followed by nitrite oxidation by nitrite-oxidizing bacteria. One of the representatives of the AOB group is Nitrosomonas mobilis species. Although a few pure strains of this species have been isolated so far, approaches to their preservation in pure culture have not been established. Here, we report isolation of novel members of the N. mobilis species from autotrophic nitrifying granules used for ammonia-rich wastewater treatment. We developed an isolation method focusing on microcolonies formation of nitrifying bacteria. Two kinds of distinctive light scattering signatures in a cell-sorting system enabled to separate microcolonies from single cells and heterogeneous aggregates within granule samples. Inoculation of a pure microcolony into 96-well microtiter plates led to successful subculturing and increased probability of isolation. Obtained strain Ms1 is cultivated in the liquid culture with relatively high ammonia or nitrite concentration, not extremely slow growing. Considering environmental clones that were closely related to N. mobilis and detected in various environments, the availability of this novel strain would facilitate to reveal this member's ecophysiology in a variety of habitats.

    DOI: 10.3389/fmicb.2015.01159

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  • A novel cultivation and isolation strategy for uncultured nitrite-oxidizing bacteria of the genus Nitrospira Invited Reviewed

    Hirotsugu Fujitani, Satoshi Tsuneda

    Journal of Environmental Biotechnology   14 ( 2 )   99 - 103   2015.3

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  • Isolation of sublineage I Nitrospira by a novel cultivation strategy Reviewed

    Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda, Yoshiteru Aoi

    Environmental Microbiology   16 ( 10 )   3030 - 3040   2014.10

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    Nitrification is an important process in the biogeochemical nitrogen cycle and is widely exploited in biological wastewater treatment. Recently, Nitrospira has been recognized as the numerically dominant nitrite-oxidizing bacterial genus and is primarily responsible for the second step of aerobic nitrification. Nevertheless, the physiological properties of Nitrospira remain poorly understood because the organisms are difficult to isolate and culture. Here, we report a novel cultivation strategy for obtaining members of the Nitrospira sublineage I in pure culture. The method combines: (i) selective enrichment of Nitrospira using a continuous feeding reactor and (ii) purification followed by sub-cultivation via a cell sorting system by focusing on the unique characteristics of Nitrospira forming spherical micro-colonies. This strategy is potentially applicable to other uncultured or unisolated Nitrospira and could accelerate the physiological and biochemical understandings of this important group of organisms.

    DOI: 10.1111/1462-2920.12248

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  • Isolation of Nitrospira belonging to Sublineage II from a Wastewater Treatment Plant Reviewed

    Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    Microbes and Environments   28 ( 3 )   346 - 353   2013.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPANESE SOC MICROBIAL ECOLOGY, DEPT BIORESOURCE SCIENCE  

    Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitrite-oxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

    DOI: 10.1264/jsme2.ME13042

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  • Selective enrichment of two different types of Nitrospira-like nitrite-oxidizing bacteria from a wastewater treatment plant Reviewed

    Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    Microbes and Environments   28 ( 2 )   236 - 243   2013.6

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    Nitrification is an important step in nitrogen removal in biological wastewater treatment processes. Recently, Nitrospira have been recognized as the numerically dominant nitrite-oxidizing bacterial genus primarily responsible for the second step of aerobic nitrification
    however, Nitrospira usually resist cultivation under laboratory conditions and only one species enriched from activated sludge has been described. In this study, a novel enrichment method for Nitrospira was successfully developed using continuous feeding bioreactors. By controlling nitrite concentrations strictly in the bioreactor at low levels below 10 mg-N L-1, coexisting members of sublineages I and II of the genus Nitrospira were enriched selectively. The maximum ratios of sublineages I and II to total microbial cells achieved 88.3% and 53.8%, respectively. This enrichment method is potentially applicable to other uncultured Nitrospira.

    DOI: 10.1264/jsme2.ME12209

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Presentations

  • Different gene expression between high and low temperatures of a nitrite-oxidizing bacterium Nitrobacter sp. strain CN101

    Yuki Shiraishi, Yuichi Suwa, Hirotsugu Fujitani

    36th Japanese Society Microbial Ecology Annual Meeting and 13th Asian Symposium on Microbial Ecology  2023.11 

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    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Phylogeny and physiology of an acid-tolerant ammonia-oxidizing bacterium Nitrosospira coexisting in comammox enrichment

    Rikako Miyata, Takashi Mitsuboshi, Tatsuo Sumino, Yuichi Suwa, Hirotsugu Fujitani

    36th Japanese Society Microbial Ecology Annual Meeting and 13th Asian Symposium on Microbial Ecology  2023.11 

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  • Distinct N2O production dynamics by cluster 7 ammonia-oxidizing bacterial isolates from partial nitrifying bioreactors: Genomic and physiological insights

    Akito Oishi, Satoru Ono, Toshikazu Suenaga, Hirotsugu Fujitani, Yuichi Suwa, Megumi Kuroiwa, Akihiko Terada

    36th Japanese Society Microbial Ecology Annual Meeting and 13th Asian Symposium on Microbial Ecology  2023.11 

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  • A novel nitrite-oxidizing Nitrobacter sp. strain A67 isolated from agricultural soil adapts to moderately acidic pH

    Ayano Kaneko, Yuta Endo, Takuya Ninomiya, Megumi Kuroiwa, Hirotsugu Fujitani, Yuichi Suwa

    36th Japanese Society Microbial Ecology Annual Meeting and 13th Asian Symposium on Microbial Ecology  2023.11 

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  • Individual-level phenotypic heterogeneity in ammonia-oxidizing bacteria revealed by single-cell observation

    Shuto Ikeda, Hirotsugu Fujitani, Satoshi Tsuneda

    36th Japanese Society Microbial Ecology Annual Meeting and 13th Asian Symposium on Microbial Ecology  2023.11 

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  • Effects and significance of cell-cell growth individuality in ammonia-oxidizing bacteria

    Rino Isshiki, Hirotsugu Fujitani, Satoshi Tsuneda

    2023.11 

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  • Obtaining laboratory cultures of acid-torelant comammox bacteria from arable soil

    Hinano Furuya, Hirotsugu Fujitani, Yuichi Suwa

    2023.11 

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  • Enriching acid-tolerant nitrite-oxidizing bacteria from acidic soil

    Rina Murata, Mayu Kikuchi, Hirotsugu Fujitani, Yuichi Suwa

    2023.11 

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  • An attempt to determine gross N2O emission rate from soil using 15N-labeled N2O

    Chihiro Shimizu, Yuri Ohtomo, Megumi Kuroiwa, Hirotsugu Fujitani, Shin-ichi Tokuda, Masahito Hayatsu, Kaneko Tago, Yuichi Suwa

    2023.11 

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  • Influence of incubation time on gross nitrification rates of arable soil

    Yuri Ohtomo, Megumi Kuroiwa, Hirotsugu Fujitani, Shin-ichi Tokuda, Masahito Hayatsu, Kaneko Tago, Yuichi Suwa

    2023.11 

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  • Isolation strategy for acid-tolerant/acidophilic comammox ~2. Obtaining comammox batch culture in inorganic medium~

    Sota Nakamura, Takashi Mitsuboshi, Tatsuo Sumino, Yuichi Suwa, Hirotsugu Fujitani

    36th Japanese Society Microbial Ecology Annual Meeting and 13th Asian Symposium on Microbial Ecology  2023.11 

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    Language:Japanese   Presentation type:Oral presentation (general)  

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  • Genomic and physiological insights into N2O emissions by cluster 7 ammonia-oxidizing bacteria isolated from partial nitrifying bioreactors

    Akito Oishi, Toshikazu Suenaga, Hirotsugu Fujitani, Yuichi Suwa, Megumi Kuroiwa, Akihiko Terada

    8th International Conference on Nitrification  2023.7 

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    Event date: 2023.7 - 2023.8

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  • Growth heterogeneity of divided cells affects the culturability of ammonia-oxidizing bacteria

    Shuto Ikeda, Hirotsugu Fujitani, Satoshi Tsuneda

    8th International Conference on Nitrification  2023.7 

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    Event date: 2023.7 - 2023.8

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  • Temperature dependence in nitrite oxidation and gene expression by a Nitrobacter sp.

    Yuki Shiraishi, Yuichi Suwa, Hirotsugu Fujitani

    8th International Conference on Nitrification  2023.7 

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    Event date: 2023.7 - 2023.8

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  • Isolation and physiological characterization of a novel nitrite-oxidizing bacterium, Nitrobacter sp. A67 which accommodates to moderately acidic pH from agricultural soil

    Ayano Kaneko, Yuta Endo, Takuya Ninomiya, Megumi Kuroiwa, Hirotsugu Fujitani, Yuichi Suwa

    8th International Conference on Nitrification  2023.7 

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    Event date: 2023.7 - 2023.8

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  • シングルセル観察で解き明かすアンモニア酸化細菌の分裂速度不均一性

    池田 秀斗, 藤谷 拓嗣, 常田 聡

    第11回日本生物工学会東日本支部コロキウム  2023.3 

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  • アンモニア酸化細菌Nitrosomonasにおける個体レベルの表現型不均一性の解析

    池田 秀斗, 一色 理乃, 藤谷 拓嗣, 常田 聡

    日本微生物生態学会第35回大会  2022.11 

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    Event date: 2022.10 - 2022.11

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  • シングルドロップレット分取技術を用いた環境微生物の培養

    野田 尚宏, 大田 悠里, 斉藤 加奈子, Dieter M Tourlousse, 高木 妙子, 松倉 智子, 森田 雅宗, 藤谷 拓嗣, 常田 聡

    日本微生物生態学会第35回大会  2022.11 

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  • アンモニア酸化細菌3株における難培養性と増殖速度のばらつきの比較

    一色 理乃, 藤谷 拓嗣, 常田 聡

    日本微生物生態学会第35回大会  2022.11 

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  • 耐酸性comammoxの分離戦略

    三星 峻, 角野 立夫, 諏訪 裕一, 藤谷 拓嗣

    日本微生物生態学会第35回大会  2022.11 

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  • Bacterial growth heterogeneity causes persistent infection and unculturability in environment

    Rino Isshiki, Naoki Yamamoto, Yuto Kawai, Hirotsugu Fujitani, Satoshi Tsuneda

    The 15th Korea-Japan Symposium on Microbiology  2022.10 

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  • Combination of droplet based cultivation and single droplet sorting paves the way for isolating slow growers in soil

    Yuri Ota, Kanako Saito, Dieter Tourlousse, Taeko Takagi, Satoko Matsukura, Masamune Morita, Hirotsugu Fujitani, Satoshi Tsuneda, Naohiro Noda

    18th International society for microbial ecology  2022.8 

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  • Genomics of acid adapted comammox and nitrite-oxidizing Nitrospira: are they specialists on low pH or generalists surviving a broad range of pH?

    Yu Takahashi, Hirotsugu Fujitani, Itsuki Tanguchi, Tetsuya Hayashi, Kanako Tago, Masahito Hayatsu, Satoshi Tsuneda

    18th International society for microbial ecology  2022.8 

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  • Comparison of three strains of ammonia-oxidizing bacteria to reveal the relationship between culturability and growth heterogeneity

    Rino Isshiki, Hirotsugu Fujitani, Satoshi Tsuneda

    18th International society for microbial ecology  2022.8 

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  • 活性化エネルギーから見る亜硝酸酸化細菌の温度依存性の比較

    白石 夕貴, 諏訪 裕一, 藤谷 拓嗣

    日本微生物生態学会第34回大会  2021.10 

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    Event date: 2021.10 - 2021.11

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  • 亜硝酸に対して親和性の高い土壌亜硝酸酸化細菌Nitrobacter sp. CN101の生理学とゲノム科学

    小林 陽一郎, 二宮 拓也, 藤谷 拓嗣, 黒岩 恵, 諏訪 裕一

    日本微生物生態学会第34回大会  2021.10 

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  • 好酸性亜硝酸酸化細菌Nitrobacter sp. A67株の分離と生理学的特徴づけー硝化コミュニティーの唯一のエネルギー源として尿素を用いた硝化菌の分離戦略―

    遠藤 優太, 藤谷 拓嗣, 二宮 拓也, 小林 陽一郎, 黒岩 恵, 諏訪 裕一

    日本微生物生態学会第34回大会  2021.10 

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  • 酸性条件下における硝化の担い手は誰か

    三星 峻, 角野 立夫, 遠藤 優太, 諏訪 裕一, 藤谷 拓嗣

    日本微生物生態学会第34回大会  2021.10 

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  • 土壌抽出液培地による新規な硝化菌培養の可能性2〜酸性森林土壌の場合〜

    菊地 麻友, 高山 祐子, 三星 峻, 梅澤 千尋, 高橋 昌平, 古屋 日向乃, 黒岩 恵, 磯部 一夫, 藤谷 拓嗣, 諏訪 裕一

    日本微生物生態学会第34回大会  2021.10 

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  • 好酸性NOB Nitrobacter sp. A67と共存するアンモニア酸化アーキアの集積培養

    高山 祐子, 遠藤 優太, 二宮 拓也, 黒岩 恵, 藤谷 拓嗣, 諏訪 裕一

    日本微生物生態学会第34回大会  2021.10 

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  • 弱酸性硝化リアクターで集積培養された新規なcomammox Nitrospiraのコンプリートゲノムの構築

    髙橋 悠, 藤谷 拓嗣, 谷口 愛樹, 林 哲也, 多胡 香奈子, 早津 雅仁, 常田 聡

    日本微生物生態学会第34回大会  2021.10 

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  • 亜硝酸酸化細菌Nitrospiraにおける2種類のMazEF機構の機能解析

    葵 理恵, 宮本 龍樹, 岡部 拓真, 横田 亜紀子, 大田 悠里, 藤谷 拓嗣, 常田 聡, 野田 尚宏

    日本微生物生態学会第34回大会  2021.10 

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  • 環境微生物獲得のためのwater-in-oilドロップレット培養手法の構築

    大田 悠里, 斉藤 加奈子, Dieter M Tourlousse, 松倉 智子, 高木 妙子, 藤谷 拓嗣, 森田 雅宗, 常田 聡, 野田 尚宏

    環境バイオテクノロジー学会2021年度大会  2021.9 

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  • 茶園の酸性土壌に生息する硝化菌Nitrospiraの集積培養

    髙橋 悠, 藤谷 拓嗣, 廣野 祐平, 多胡 香奈子, 早津 雅仁, 常田 聡

    環境バイオテクノロジー学会2021年度大会  2021.9 

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  • 未培養硝化菌の分離戦略と生理生態 Invited

    藤谷拓嗣

    環境バイオテクノロジー学会2021年度大会  2021.9 

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  • NitrospiraにおけるToxin-Antitoxin機構の生理学的意義の解明

    葵 理恵, 宮本 龍樹, 岡部 拓真, 横田 亜紀子, 大田 悠里, 藤谷 拓嗣, 常田 聡, 野田 尚宏

    第15回細菌学若手コロッセウム  2021.8 

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  • Water-in-oilドロップレットを用いた環境中の微生物獲得手法の構築

    大田 悠里, 斉藤 加奈子, Dieter M Tourlousse, 松倉 智子, 高木 妙子, 藤谷 拓嗣, 森田 雅宗, 常田 聡, 野田 尚宏

    第15回細菌学若手コロッセウム  2021.8 

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  • 地球の窒素循環を駆動する未培養微生物の実態に迫る Invited

    藤谷拓嗣

    第15回細菌学若手コロッセウム  2021.8 

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  • Genomics and physiology of novel Nitrobacter strain adapted to an acidic soil environment of heavily fertilized tea field

    Yu Takahashi, Kento Ishii, Hirotsugu Fujitani, Toshikazu Suenaga, Akihiko Terada, Yuji Sekiguchi, Kanako Tago, Masahito Hayatsu, Yuhei Hirono, Satoshi Tsuneda

    7th International Conference on Nitrification  2021.7 

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  • Characterization of Nitrobacter sp. CN101 with highly affinity to nitrite isolated from mixed culture with urea from Japanese forest soil

    Yoichiro Kobayashi, Takuya Ninomiya, Hirotsugu Fujitani, Yuichi Suwa

    7th International Conference on Nitrification  2021.7 

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  • Isolation of an aciophilic nitrite-oxidizing bacterium, Nitrobacter sp. strain A67, using culture medium containing urea as a sole source of energy for nitrifying community

    Yuta Endo, Takuya Ninomiya, Hirotsugu Fujitani, Yuichi Suwa

    7th International Conference on Nitrification  2021.7 

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  • Comparative physiology and genome of ammonia-oxidizing Nitrosospira isolated from terrestrial and freshwater environments International conference

    Emi Nissato, Hirotsugu Fujitani, Keisuke Sakai, Hiroshi Ishikawa, Yuichi Suwa

    7th International Conference on Nitrification  2021.7 

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  • 亜硝酸酸化細菌Nitrobacterの新株が持つ固有の窒素代謝経路と亜酸化窒素還元機能の発見

    髙橋 悠, 石井 拳人, 藤谷 拓嗣, 関口 勇地, 末永 俊和, 寺田 昭彦, 廣野 祐平, 多胡 香奈子, 早津 雅仁, 常田 聡

    第15回日本ゲノム微生物学会年会  2021.3 

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  • 独立栄養性亜硝酸酸化細菌のプロテオームに対する有機物の影響

    石井 拳人, 藤谷 拓嗣, Barth F. Smets, 常田 聡

    第15回日本ゲノム微生物学会年会  2021.3 

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  • 網羅的遺伝子発現解析によって見えてきたアンモニア酸化細菌の凝集体と非凝集体の分業形態

    一色 理乃, 藤谷 拓嗣, 常田 聡

    第14回日本ゲノム微生物学会年会  2020.3 

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  • 新規な分離培養技術が紐解く未知微生物:硝化菌の生き様とは?

    SATテクノロジーショーケース2020  2020.1 

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  • Genomic and physiological characteristics of novel nitrite-oxidizing Nitrospira strain isolated from a drinking water treatment plant International conference

    Hirotsugu Fujitani, Kengo Momiuchi, Kento Ishii, Masaru Jinno, Norisuke Ushiki, Yuji Sekiguchi, Satoshi Tsuneda

    7th International Conference Microbial Ecology and Water Engineering  2019.11 

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  • Enrichment of comammox Nitrospira from nitrifying granules by using fixed-bed continuous feeding bioreactors International conference

    Hirotsugu Fujitani, Manami Nomachi, Yu Takahashi, Yoshiaki Hasebe, Masahiro Eguchi, Satoshi Tsuneda

    7th International Conference Microbial Ecology and Water Engineering  2019.11 

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  • A flexible survival strategy of ammonia-oxidizing bacteria by growth heterogeneity at a single cell level

    Rino Isshiki, Hirotsugu Fujitani, Daiki Tanaka, Tetsushi Sekiguchi, Satoshi Tsuneda

    6th International Conference on Nitrification  2019.10 

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  • Enrichment of comammox and canonical Nitrospira from acidic soil: Insights into niche differentiation and adaptation to low pH

    Yu Takahashi, Hirotsugu Fujitani, Yuhei Hirono, Kanako Tago, Yong Wang, Masahito Hayatsu, Satoshi Tsuneda

    6th International Conference on Nitrification  2019.10 

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  • Physiological and Genomic Characteristics of a New “Candidatus Nitrotoga” Isolate

    Kento Ishii, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    6th International Conference on Nitrification  2019.10 

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  • Sequence-specific endoribonucleolytic toxin, MazF, conserved in nitrifying bacteria

    Rie Aoi, Tatsuki Miyamoto, Akiko Yokota, Yuri Ota, Hirotsugu Fujitani, Satoshi Tsuneda, Naohiro Noda

    6th International Conference on Nitrification  2019.10 

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  • Enrichment of comammox Nitrospira from nitrifying granules by using fixed-bed continuous feeding bioreactors International conference

    Hirotsugu Fujitani, Manami Nomachi, Yu Takahashi, Yoshiaki Hasebe, Masahiro Eguchi, Satoshi Tsuneda

    6th International Conference on Nitrification  2019.10 

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  • Characterization of a bacterium representing a previously unrecognized clade of the Nitrosomonas group; physiology, genomics, and distribution of a missing ammonia-oxidizer International conference

    Hirotsugu Fujitani, Shuta Kikuchi, Kento Ishii, Yuji Sekiguchi, Satoshi Tsuneda

    6th International Conference on Nitrification  2019.10 

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  • Metabolic Lifestyles of Nitrite Oxidizer Nitrotoga under Mixotrophic and Heterotrophic Conditions

    Kento Ishii, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    2019 Nordic Meeting in Molecular Microbial Ecology  2019.9 

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  • 従属栄養細菌との相互作用を介した硝化菌の増殖促進機構

    井出 寛人, 石井 拳人, 藤谷 拓嗣, 常田 聡

    日本微生物生態学会第33回大会  2019.9 

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  • 亜酸化窒素還元能を持つ硝化細菌Nitrobacterの発見

    髙橋 悠, 藤谷 拓嗣, 末永 俊和, 寺田 昭彦, 廣野 祐平, 多胡 香奈子, 早津 雅仁, 常田 聡

    日本微生物生態学会第33回大会  2019.9 

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  • 培養技術とゲノム解析で紐解く硝化菌の生理生態 Invited

    藤谷 拓嗣

    第22回日本水環境学会シンポジウム  2019.9 

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  • Mixotrophic Lifestyle of Nitrite Oxidizer Nitrotoga in the Presence of Pyruvate

    Kento Ishii, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    8th Congress of European Microbiologists  2019.7 

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  • Cultivation of a bacterium representing a previously unrecognized clade of the Nitrosomonas group; physiology, genomics, and distribution of a missing ammonia-oxidizer International conference

    Hirotsugu Fujitani, Shuta Kikuchi, Kento Ishii, Yuji Sekiguchi, Satoshi Tsuneda

    8th Congress of European Microbiologists  2019.7 

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  • 未培養硝化菌を対象とした新規分離培養技術の開発

    藤谷拓嗣, 常田聡

    環境バイオテクノロジー学会2019年度大会  2019.6 

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  • Enrichment of Complete Ammonia Oxidizer and Nitrite Oxidizer of genus Nitrospira from Acidic Soil

    Yu Takahashi, Hirotsugu Fujitani, Kanako Tago, Yong Wang, Yuhei Hirono, Masahito Hayatsu, Satoshi Tsuneda

    11th Asian Symposium on Microbial Ecology  2019.5 

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  • ピルビン酸存在下での亜硝酸酸化細菌Nitrotogaの混合栄養的な増殖様式

    石井 拳人, 藤谷 拓嗣, 関口勇地, 常田 聡

    第13回日本ゲノム微生物学会年会  2019.3 

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  • 網羅的遺伝子発現解析による硝化菌の形態変化がもたらす生存戦略の解明

    一色 理乃, 藤谷 拓嗣, 田中 大器, 関口 哲志, 常田 聡

    第13回日本ゲノム微生物学会年会  2019.3 

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  • 見過ごされたアンモニア酸化細菌:Nitrosomonas属の新規な生理学,ゲノミクス,環境分布

    藤谷拓嗣, 菊池脩太, 石井拳人, 関口勇地, 常田聡

    第13回日本ゲノム微生物学会年会  2019.3 

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  • Culture-dependent analysis of isolated Nitrotoga sp. provide deeper insight into the physiology

    Kento Ishii, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    17th International society for microbial ecology  2018.8 

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  • Growth variability at the single-cell level affects culturability of environmental microorganisms

    Rino Isshiki, Hirotsugu Fujitani, Daiki Tanaka, Tetsushi Sekiguchi, Satoshi Tsuneda

    17th International society for microbial ecology  2018.8 

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  • Genomic and physiological characteristics of novel nitrite-oxidizing Nitrospira isolated from a drinking water treatment plant International conference

    Hirotsugu Fujitani, Kengo Momiuchi, Kento Ishii, Masaru Jinno, Norisuke Ushiki, Yuji Sekiguchi, Satoshi Tsuneda

    17th International society for microbial ecology  2018.8 

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  • Cultivation of novel nitrite oxidizer Nitrospira from acidic soil

    Yu Takahashi, Hirotsugu Fujitani, Yuhei Hirono, Masahito Hayatsu, Satoshi Tsuneda

    32th Japanese Society Microbial Ecology Annual Meeting and 10th Asian Symposium on Microbial Ecology  2018.7 

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  • Growth heterogeneity in pure cultures affects the culturability

    Rino Isshiki, Hirotsugu Fujitani, Daiki Tanaka, Tetsushi Sekiguchi, Satoshi Tsuneda

    32th Japanese Society Microbial Ecology Annual Meeting and 10th Asian Symposium on Microbial Ecology  2018.7 

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  • Isolation process and genomic analysis of nitrite oxidizer Nitrotoga sp. provide insights on physiological characteristics and clues to promote the growth

    Kento Ishii, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    32th Japanese Society Microbial Ecology Annual Meeting and 10th Asian Symposium on Microbial Ecology  2018.7 

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  • N2Oを固定するシアノバクテリアの探索とポテンシャル評価

    須田和美, 松崎壮一郎, 末永俊和, 利谷翔平, 藤谷拓嗣, 常田聡, 細見正明, 寺田昭彦

    第52回日本水環境学会年会  2018.3 

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  • High denitrification potentials of soils under seabird cliffs: Case studies in Ny-Ålesund, Svalbard

    Kentaro Hayashi, Yukiko Tanabe, Keisuke Ono, Maarten J, J. E. Loonen, Maki Asano, Hirotsugu Fujitani, Masaki Uchiba, Masahito Hayatsu

    8th Symposium on Polar Science: Polar Biology  2017.12 

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  • 完全アンモニア酸化能を保持するComammox細菌の分離培養と生理生態

    野町 真名実, 藤谷 拓嗣, 長谷部 吉昭, 江口 正浩, 常田 聡

    環境微生物系学会合同大会2017  2017.8 

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  • ゲノムから推定する遅増殖性Nitrotogaの活性促進因子

    石井 拳人, 藤谷 拓嗣, 常田 聡

    環境微生物系学会合同大会2017  2017.8 

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  • 硝化菌単一菌株集団でみられる形態と増殖能の不均一性

    一色 理乃, 藤谷 拓嗣, 田中 大器, 関口 哲志, 常田 聡

    環境微生物系学会合同大会2017  2017.8 

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  • Genome-informed isolation of nitrite oxidizer Nitrotoga sp

    Kento Ishii, Hirotsugu Fujitani, Tatsunori Nakagawa, Reiji Takahashi, Satoshi Tsuneda

    5th International Conference on Nitrification  2017.7 

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  • Growth heterogeneity in pure cultures of nitrifiers

    Rino Isshiki, Hirotsugu Fujitani, Daiki Tanaka, Tetsushi Sekiguchi, Satoshi Tsuneda

    5th International Conference on Nitrification  2017.7 

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  • Comparative genomics of phylogenetically distinct two Nitrospira strains isolated from activated sludge International conference

    Norisuke Ushiki, Hirotsugu Fujitani, Yu Shimada, Tomohiro Morohoshi, Yuji Sekiguchi, Satoshi Tsuneda

    5th International Conference on Nitrification  2017.7 

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  • 革新的分離培養技術による新規なcomammox細菌の獲得と生存戦略の解明

    野町真名実, 藤谷拓嗣, 長谷部吉昭, 江口正浩, 常田 聡

    第19回化学工学会学生発表会  2017.3 

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  • セルソーターによって分取された凝集体内の微生物間相互作用の解明

    石井拳人, 藤谷拓嗣, 常田 聡

    第11回日本ゲノム微生物学会年会  2017.3 

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  • Nitrospiraの亜硝酸酸化活性を制御するQuorum-sensing機構の発見

    牛木章友, 藤谷拓嗣, 諸星知広, 常田 聡

    第11回日本ゲノム微生物学会年会  2017.3 

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  • 亜酸化窒素の削減に寄与する淡水性シアノバクテリアの探索

    松崎壮一郎, 末永俊和, 利谷翔平, 堀知行, 牛木章友, 藤谷拓嗣, 常田聡, 細見正明, 寺田昭彦

    第5回日本生物工学会東日本支部コロキウム  2017.3 

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  • 微生物凝集体ソーティングによる未培養な亜硝酸酸化細菌Nitrotogaの獲得と生存戦略

    石井拳人, 藤谷拓嗣, 中川達功, 高橋令二, 常田 聡

    日本微生物生態学会第31回大会  2016.10 

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  • Quorum-sensing機構による亜硝酸酸化細菌Nitrospira japonicaの活性制御メカニズム

    牛木章友, 島田 侑, 藤谷拓嗣, 諸星知広, 常田 聡

    日本微生物生態学会第31回大会  2016.10 

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  • クローナルな硝化菌集団における細胞増殖活性の不均一性の評価と機構解明

    一色理乃, 藤谷拓嗣, 田中大器, 関口 哲志, 常田 聡

    日本微生物生態学会第31回大会  2016.10 

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  • Ammonia concentration determines ecological niche differentiation of Nitrospira as nitrite-oxidizers in activated sludge International conference

    Hirotsugu Fujitani, Masaru Jinno, Norisuke Ushiki, Toshikazu Suenaga, Akihiko Terada, Satoshi Tsuneda

    6th International Conference Microbial Ecology and Water Engineering  2016.9 

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  • Physiological and genetic properties of Nitrosomonas mobilis Ms1 isolated from autotrophic nitrifying granule of wastewater treatment plant

    Soe Myat Thandar, Norisuke Ushiki, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    16th International society for microbial ecology  2016.8 

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  • Comparative genomic analysis of phylogenetically distinct two Nitrospira strains isolated from a wastewater treatment plant

    Norisuke Ushiki, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    16th International society for microbial ecology  2016.8 

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  • Direct isolation of microcolonies from activated sludge opens the window of unrecognized genus Nitrosomonas as ammonia oxidizers International conference

    Hirotsugu Fujitani, Takuma Abe, Norisuke Ushiki, Satoshi Tsuneda

    16th International society for microbial ecology  2016.8 

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  • 新規な分離培養技術によって獲得された硝化細菌の生理・ゲノム特性

    藤谷拓嗣, 牛木章友, 神野 大, 安部拓磨, 常田 聡

    環境バイオテクノロジー学会2016年度大会  2016.6 

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  • Physiological and genomic characteristics of uncultured nitrifiers illuminated by novel cultivation and isolation strategies Invited

    Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda

    2016.3 

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  • 淡水域に棲息する N2O 同化を行う Cyanobacteria の探索

    松崎壮一郎, 末永俊和, 堀知行, 牛木章友, 藤谷拓嗣, 常田聡, 利谷翔平, 細見正明, 寺田昭彦

    第50回日本水環境学会年会  2016.3 

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  • Physiological characterization of Nitrotoga-like bacteria enriched from coastal sand

    Kento Ishii, Hirotsugu Fujitani, Tatsunori Takahashi, Reiji Takahashi, Satoshi Tsuneda

    16th International society for microbial ecology  2016.3 

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  • 比較ゲノム解析が明らかにする亜硝酸酸化細菌Nitrospira の生態学的ニッチ

    牛木章友, 藤谷拓嗣, 関口勇地, 常田 聡

    31th Japanese Society Microbial Ecology Annual Meeting and 7th Japan-Taiwan-Korea Symposium on Microbial Ecology  2015.10 

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  • Physiological characterization of Nitrosomonas mobilis-like strain isolated from autotrophic nitrifying granule

    Soe Myat Thandar, Norisuke Ushiki, Hirotsugu Fujitani, Satoshi Tsuneda

    31th Japanese Society Microbial Ecology Annual Meeting and 7th Japan-Taiwan-Korea Symposium on Microbial Ecology  2015.10 

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  • 活性汚泥中に潜在する未培養アンモニア酸化細菌の分離培養

    安部拓磨, 牛木章友, 藤谷拓嗣, 常田 聡

    31th Japanese Society Microbial Ecology Annual Meeting and 7th Japan-Taiwan-Korea Symposium on Microbial Ecology  2015.10 

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  • Isolation, physiology, and genomics of novel nitrifiers Invited International conference

    Hirotsugu Fujitani, Satoshi Tsuneda

    31th Japanese Society Microbial Ecology Annual Meeting and 7th Japan-Taiwan-Korea Symposium on Microbial Ecology  2015.10 

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  • Selective enrichment of uncultured ammonia-oxidizing bacteria and archaea and Nitrospira from freshwater by continuous feeding bioreactors

    Kengo Momiuchi, Hirotsugu Fujitani, Satoshi Tsuneda

    4th International Conference on Nitrification  2015.6 

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  • Comparative genomic analysis of phylogenetically distant two Nitrospira strains isolated from a wastewater treatment plant

    Norisuke Ushiki, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    4th International Conference on Nitrification  2015.6 

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  • 新規分離培養手法を用いたハイスループットなアンモニア酸化細菌の獲得

    安部拓磨, 牛木章友, 藤谷拓嗣, 常田 聡

    2015年度環境バイオテクノロジー学会  2015.6 

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  • Isolation and successful subculture of Nitrosomonas mobilis lineage: Recovery of nature's lost treasure since 1970's International conference

    Hirotsugu Fujitani, Asami Kumagai, Norisuke Ushiki, Kengo Momiuchi, Satoshi Tsuneda

    4th International Conference on Nitrification  2015.6 

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  • 難培養性アンモニア酸化細菌Nitrosomonas mobilisの分離培養と生理学的性質の解明

    熊谷麻美, 籾内研吾, 藤谷拓嗣, 常田 聡

    環境微生物系合同大会2014  2014.10 

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  • 比較解析から迫る亜硝酸酸化細菌Nitrospiraの生態学的ニッチ

    神野 大, 牛木章友, 藤谷拓嗣, 常田 聡

    環境微生物系合同大会2014  2014.10 

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  • nxrBを標的としたin situ RCA-FISH法による亜硝酸酸化細菌の探索

    牛木章友, 藤谷拓嗣, 星野辰彦, 常田 聡

    環境微生物系合同大会2014  2014.10 

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  • Selective enrichment of uncultured ammonia-oxidizing bacteria and archaea and nitrite oxidizing bacteria from freshwater

    Kengo Momiuchi, Hirotsugu Fujitani, Satoshi Tsuneda

    15th International society for microbial ecology  2014.8 

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  • Detection of nxrB genes by in situ RCA-FISH to identify unrecognized nitrite-oxidizing microorganisms in environments

    Norisuke Ushiki, Hirotsugu Fujitani, Tatsuhiko Hoshino, Satoshi Tsuneda

    15th International society for microbial ecology  2014.8 

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  • Selective enrichment of ammonia-oxidizing archaea and bacteria from freshwater

    Kengo Momiuchi, Hirotsugu Fujitani, Satoshi Tsuneda

    Water and Environment Technology Conference 2014  2014.6 

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  • 排水処理槽内から単離された2種類のNitrospiraの比較解析

    牛木章友, 藤谷拓嗣, 常田 聡

    第48回日本水環境学会年会  2014.3 

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  • 機能遺伝子nxrBに基づく新規な亜硝酸酸化細菌の探索

    牛木章友, 藤谷拓嗣, 常田 聡

    日本微生物生態学会第29回大会  2013.11 

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  • 低濃度アンモニアの連続供給による難培養性硝化微生物の選択的集積培養

    籾内研吾,藤谷拓嗣,常田 聡

    日本微生物生態学会第29回大会  2013.11 

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  • 亜硝酸酸化細菌Nitrospiraを標的とした新規分離培養手法と他の難培養性微生物への適用

    藤谷拓嗣, 籾内研吾, 牛木章友, 入江香菜, 熊谷麻美, 安部拓磨, 神野 大, 青井議輝, 常田 聡

    日本微生物生態学会第29回大会  2013.11 

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  • 難培養性亜硝酸酸化細菌Nitrospiraを標的とした革新的分離培養戦略

    藤谷拓嗣, 牛木章友, 籾内研吾, 青井議輝, 常田 聡

    第16回日本水環境学会シンポジウム  2013.11 

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  • Selective inoculation of microcolonies materializes cultivation and isolation of previously uncultured nitrifying bacteria International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo momiuchi, Asami Kumagai, Takuma Abe, Satoshi Tsuneda

    The 5th Taiwan-Korea-Japan International Symposium on Microbial Ecology  2013.10 

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  • Comparative physiological analysis of previously uncultured Nitrospira strains isolated from wastewater treatment plants

    Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    3rd International conference on nitrification  2013.9 

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    Event date: 2013.9    

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  • An innovative cultivation and isolation strategy for uncultured nitrifying bacteria International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo Momiuchi, Yoshiteru Aoi, Satoshi Tsuneda

    3rd International conference on nitrification  2013.9 

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  • An innovative cultivation and isolation strategy for uncultured nitrifying bacteria International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo Momiuchi, Yoshiteru Aoi, Satoshi Tsuneda

    Early career scientist workshop ICoN3  2013.9 

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  • An innovative cultivation and isolation strategy for uncultured nitrite-oxidizing bacteria of the genus Nitrospira International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo Momiuchi, Yoshiteru Aoi, Satoshi Tsuneda

    5th International Conference Microbial Ecology and Water Engineering  2013.7 

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    Event date: 2013.7    

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  • An innovative cultivation and isolation strategy for uncultured nitrifying bacteria International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo momiuchi, Yoshiteru Aoi, Satoshi Tsuneda

    CER International workshop on Biogeochemical cycling and Microbial Ecology for Young Scientists  2013.3 

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  • 淡水環境に生息する難培養性硝化細菌の新規分離培養手法の開発

    籾内研吾, 藤谷拓嗣, 青井議輝, 常田 聡

    第47回日本水環境学会  2013.3 

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  • 出芽酵母と共凝集する乳酸菌の細胞表面特性に関する研究

    平山 悟, 臼井有美, 土屋典子, 阿部 侑, 籾内研吾, 藤谷拓嗣, 常田 聡, 古川壮一, 荻原博和, 森永 康

    第64回生物工学会大会  2012.10 

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  • 培養できない微生物を培養するための新規技術開発 Invited

    青井議輝, 藤谷拓嗣, 常田 聡

    日本微生物生態学会第28回大会,第4回日韓微生物生態シンポジウム  2012.9 

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    Event date: 2012.9    

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  • Isolation of previously uncultured Nitrospira unveils the physiology of nitrite-oxidizing bacteria in wastewater treatment process

    2012.9 

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  • Selective cultivation of nitrifying bacteria present in drinking water treatment plants

    Kengo Momiuchi, Hirotsugu Fujitani, Norisuke Ushiki, Yoshiteru Aoi, Satoshi Tsuneda

    14th International society for microbial ecology  2012.8 

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  • Comparative physiological analysis of previously uncultured Nitrospira strains isolated from wastewater treatment plants

    Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    14th International society for microbial ecology  2012.8 

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  • Novel method for uncultured nitrifying bacteria Invited International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda, Yoshiteru Aoi

    14th International society for microbial ecology  2012.8 

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  • 排水処理槽内に生息する難培養性亜硝酸酸化細菌Nitrospiraの分離培養

    牛木章友, 藤谷拓嗣, 常田 聡, 青井議輝

    第14回化学工学会学生発表会  2012.3 

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  • 浄水処理プロセス由来の新規硝化細菌の分離培養

    籾内研吾, 藤谷拓嗣, 常田 聡

    日本微生物生態学会第27回大会  2011.10 

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  • 亜硝酸酸化細菌Nitrospiraの新規分離培養手法とその実証

    藤谷拓嗣, 牛木章友, 常田 聡, 青井議輝

    日本微生物生態学会第27回大会  2011.10 

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  • Selective and highly enrichment of nitrite-oxidizing bacteria Nitrospira International conference

    Hirotsugu Fujitani, Satoshi Tsuneda, Yoshiteru Aoi

    2nd International conference on nitrification  2011.7 

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  • Selective and highly enrichment of nitrite-oxidizing bacteria Nitrospira Invited International conference

    Hirotsugu Fujitani, Satoshi Tsuneda, Yoshiteru Aoi

    Early career scientist workshop ICoN2  2011.7 

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  • Growth Characteristics of Uncultured Nitrite-Oxidizing Nitrospira

    2010.11 

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  • Selective and highly efficient enrichment of uncultured Nitrospira International conference

    Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    13th International society for microbial ecology  2010.8 

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  • 難培養性亜硝酸酸化細菌Nitrospiraの分離培養

    藤谷拓嗣, 青井議輝, 常田 聡

    日本微生物生態学会第25回大会  2009.11 

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  • Cultivation of uncultured nitrite-oxidizing bacteria International conference

    Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    1st International Conference on Nitrification  2009.7 

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  • 難培養性亜硝酸酸化細菌Nitrospiraの新規分離培養法

    藤谷拓嗣, 青井議輝, 常田 聡

    第43回日本水環境学会年会  2009.3 

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  • Cultivation of uncultured nitrite oxidizing bacteria International conference

    Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    12th International society for microbial ecology  2008.8 

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  • 多摩川河口干潟の窒素循環に寄与する微生物群の解析

    藤谷拓嗣, 石川大祐, 田嶋良行, 小関祥子, 陶 景倫, 小島茂明, 浦川秀敏, 常田 聡

    第41回日本水環境学会年会  2007.3 

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Awards

  • ポスター賞(金賞)

    2023.3   第11回日本生物工学会東日本支部コロキウム   シングルセル観察で解き明かすアンモニア酸化細菌の分裂速度不均一性

    池田秀斗

  • 2020年度環境バイオテクノロジー学会奨励賞

    2020.6   Japan Society for Environmental Biotechnology  

    Hirotsugu Fujitani

  • Best Poster Award

    2019.10   6th International Conference on Nitrification   A flexible survival strategy of ammonia-oxidizing bacteria by growth heterogeneity at a single cell level

    Rino Isshiki

  • Poster Award (Second prize award)

    2019.10   6th International Conference on Nitrification   Enrichment of comammox and canonical Nitrospira from acidic soil: Insights into niche differentia-tion and adaptation to low pH

    Yu Takahashi

  • 優秀発表賞(ポスター)

    2019.9   第33回日本微生物生態学会   細胞ごとに増殖速度のばらつきが大きいアンモニア酸化細菌のしなやかな生存戦略

    一色理乃

  • 優秀発表賞(ポスター)

    2017.8   環境微生物系学会合同大会2017   ゲノムから推定する遅増殖性Nitrotogaの活性促進因子

    石井拳人

  • Best Poster Award

    2015.7   4th International Conference on Nitrification (ICoN4)   Isolation and successful subculture of Nitrosomonas mobilis lineage: Recovery of nature's lost treasure since 1970's

    FUJITANI, Hirotsugu

  • 博士研究奨励賞(オルガノ賞)

    2013.11   第16回日本水環境学会シンポジウム   難培養性亜硝酸酸化細菌Nitrospiraを標的とした革新的分離培養戦略

    藤谷 拓嗣

  • Best Poster Award

    2013.7   5th Quadrennial International Water Association Specialty Conference on Microbial Ecology and Water Engineering   An innovative cultivation and isolation strategy for uncultured nitrite-oxidizing bacteria of the genus Nitrospira

    FUJITANI, Hirotsugu

  • Poster Award

    2012.9   Isolation of previously uncultured Nitrospira unveils the physiology of nitrite-oxidizing bacteria in wastewater treatment process

    FUJITANI, Hirotsugu

  • 最優秀発表賞(ポスター)

    2009.11   第25回日本微生物生態学会   難培養性亜硝酸酸化細菌Nitrospiraの分離培養

    藤谷 拓嗣

  • 優秀発表賞(クリタ賞)

    2009.3   第43回日本水環境学会   難培養性亜硝酸酸化細菌Nitrospiraの新規分離培養法

    藤谷 拓嗣

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Research Projects

  • 好酸性comammox菌の生理学・ゲノム科学に基づく酸性硝化プロセスの実態解明

    Grant number:23H03570  2023.4 - 2027.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)  中央大学

    藤谷 拓嗣, 諏訪 裕一

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    Grant amount: \18850000 ( Direct Cost: \14500000 、 Indirect Cost: \4350000 )

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  • 好酸性亜硝酸酸化細菌Nitrobacter sp. A67株で解き明かす酸性環境での硝化菌のすがた

    Grant number:23K05923  2023.4 - 2026.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)  中央大学

    諏訪 裕一, 藤谷 拓嗣

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    Grant amount: \4680000 ( Direct Cost: \3600000 、 Indirect Cost: \1080000 )

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  • Elucidate the missing link of terrestrial nitrogen cycle &#8211; Who is the key nitrifier in acidic soil?

    Grant number:19H01156  2019.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)  National Agriculture and Food Research Organization

    多胡香奈子

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    Grant type:Competitive

    Grant amount: \44980000 ( Direct Cost: \34600000 、 Indirect Cost: \10380000 )

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  • 培養技術とゲノム解析で紐解く硝化界のダークマター「完全アンモニア酸化細菌」

    2019.4 - 2022.3

    日本学術振興会科学研究費助成事業  令和元年度若手研究 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \4160000 ( Direct Cost: \3200000 、 Indirect Cost: \960000 )

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  • 新学術領域研究令和2年度「先進ゲノム支援」

    2020.4 - 2021.3

    日本学術振興会  科学研究費助成事業 

    Hirotsugu Fujitani

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    Grant type:Competitive

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  • 2020年度国際学術誌投稿助成

    2021.2    

    中央大学 

    藤谷拓嗣

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    Grant type:Competitive

    Grant amount: \200000

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  • 選択培地と集積培養に依存しない硝化菌の純粋分離

    2018.6 - 2020.3

    日本学術振興会科学研究費助成事業  平成30年度挑戦的研究(萌芽) 

    諏訪 裕一 藤谷 拓嗣

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    Grant type:Competitive

    Grant amount: \6370000 ( Direct Cost: \4900000 、 Indirect Cost: \1470000 )

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  • 革新的分離培養技術で解明する硝化細菌の生理生態と多様性

    2016.4 - 2019.3

    日本学術振興会科学研究費助成事業  平成28年度若手研究B 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \4030000 ( Direct Cost: \3100000 、 Indirect Cost: \930000 )

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  • Succession and climate-change responses of nitrification properties of soils in a glacier foreland in the European High Arctic

    Grant number:26304018  2014.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B) 

    Hayashi Kentaro, UCHIDA Masaki, HAYATSU Masahito, Loonen Maarten J.J.E., TANABE Yukiko, ONO Keisuke, FUJITAKE Nobuhide, ASANO Maki, FUJITANI Hirotsugu, HATTORI Shohei, SHIROUZU Takashi

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    Grant amount: \17160000 ( Direct Cost: \13200000 、 Indirect Cost: \3960000 )

    Field survey on nitrification properties (ammonia oxidation potential and flora of ammonia oxidizer) of soils in a glacier foreland was conducted near Ny-Alesund, Svalbard in the European High Arctic. Immature soil in such the High Arctic showed nitrification. Relevant information utilizable for future study was derived, for example, a chronosequence is difficult to be applied such a glacier foreland due to perturbation by melting water and debris, and an open top chamber showed a passive warming effect with an 0.5 deg C increase in the mean soil temperature. Furthermore, talus soils under bird cliffs showed very high nitrification and denitrification potentials with positive temperature responses and in-situ nitrous oxide emissions.

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  • メタゲノム解析から発見された新規なComammox細菌の分離培養と性状解析

    2017.12 - 2018.11

    公益財団法人発酵研究所  平成29年度(2017年度)研究者招聘・留学助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \3000000

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  • 部分硝化の制御・安定化を志向した多様な硝化細菌の生理学的解析

    2017.10 - 2018.9

    公益財団法人クリタ水・環境科学振興財団  2017年度国内研究助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \1000000

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  • 新学術領域研究平成29年度「先進ゲノム支援」

    2017.9 - 2018.3

    日本学術振興会  科学研究費助成事業 

    藤谷 拓嗣

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    Grant type:Competitive

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  • Microbial cell aggregates are advantageous for survival under low substrate concentration

    Grant number:16K14874  2016.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research  National Agriculture and Food Research Organization

    Hayatsu Masahito, FUJITANI Hirotsugu

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    Grant amount: \3770000 ( Direct Cost: \2900000 、 Indirect Cost: \870000 )

    The purpose of this study was to shed light on the advantage of microbial cell aggregates for survival in an unfavorable soil environment such as low substrate concentration and acidic condition. Enrichment culture of acid-tolerant nitrifying bacteria isolated from tea soils was used to achieve this objective, and the following results were obtained: 1) Stable culture of acid-tolerant Nitrosoglobus cell aggregates contained 10% heterotrophic bacteria 2) Isolation of stable acid-tolerant nitrifying microbial aggregates revealed a new species of Nitrosoglobus, which was found to coexist with nitrite-oxidizer and several heterotrophic bacteria 3) Comammox, which has adapted to acidic environment, was found to be present. This bacterium coexisted with heterotrophic bacteria. These results suggested that nitrifying bacteria and certain heterotrophic bacteria coexist in aggregates and are adapted to an unfavorable soil environment such as low substrate concentration and acidic condition.

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  • 窒素循環の制御とN2O生成の抑制を志向した硝化・脱窒菌の網羅的獲得

    2017.1 - 2017.12

    公益財団法人前川報恩会  平成28年度学術研究助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \1555500

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  • 部分硝化の制御・安定化を志向した多様な硝化細菌の生理学的解析

    2016.10 - 2017.9

    公益財団法人クリタ水・環境科学振興財団  2016年度国内研究助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \930000

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  • 新学術領域研究平成28年度「先進ゲノム支援」

    2016.9 - 2017.3

    日本学術振興会  科学研究費助成事業 

    藤谷 拓嗣

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    Grant type:Competitive

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  • N2O生成に関与する硝化・脱窒細菌の網羅的遺伝子解析と分離培養

    2016.2 - 2016.12

    一般財団法人前川報恩会  平成27年度学術研究助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \1500000

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  • 環境中に潜在する硝化細菌の網羅的・高速ハンティング

    2015.7 - 2016.3

    早稲田大学  2015年度特定課題研究助成費(特定課題B) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \360000

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  • 硝化細菌のマイクロコロニー形成機構の解明

    2015.4 - 2016.3

    早稲田大学  2015年度特定課題研究助成費(基礎助成) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \240000

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  • 窒素循環に寄与する新規なアンモニア酸化細菌・古細菌の分離培養と機能解明

    2014.7 - 2015.3

    早稲田大学  2014年度特定課題研究助成費(特定課題B) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \384000

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  • nxrB遺伝子に基づく新規な亜硝酸酸化細菌の探索

    2014.4 - 2015.3

    早稲田大学  2014年度特定課題研究助成費(基礎助成) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \270000

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  • NxrB遺伝子に基づく新規な亜硝酸酸化細菌の探索・分離培養

    2014.4 - 2015.2

    公益財団法人日本科学協会  平成26年度笹川科学研究助成(学術研究部門,生物系) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \700000

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  • 未培養性アンモニア酸化細菌・古細菌を標的としたハイスループット新規分離培養手法の開発

    2013.6 - 2014.3

    早稲田大学  2013年度特定課題研究助成費(新任の教員等) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \180000

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  • 窒素循環に寄与する難培養性亜硝酸酸化細菌Nitrospiraの分離培養

    2011.4 - 2012.2

    公益財団法人日本科学協会  平成23年度笹川科学研究助成(学術研究部門,生物系) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \490000

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Other

  • 中学校・高等学校教諭専修免許状(理科)

    2009.3    

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  • 中学校・高等学校教諭1種免許状(理科)

    2007.3    

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Teaching Experience

  • 理工学基礎実験IB

  • 理工学基礎実験IA

  • 生命医科学実験I

  • 環境生物学実験

  • 環境応用微生物学

Social Activities

  • 私たちの生活に身近な微生物の世界

    Role(s): Lecturer

    公益財団法人文京アカデミー  文京アカデミア講座  2022.6 - 2022.7

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    Audience: General

    Type:Seminar, workshop

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  • Local organizing committee members

    Role(s): Organizing member

    国際水協会(IWA)  Microbial Ecology and Water Engineering 2019  2019.11    

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    Type:Seminar, workshop

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  • かくれているものは,なぁに?

    Role(s): Organizing member, Demonstrator

    国立研究開発法人産業技術総合研究所  2019年産総研一般公開  2019.7    

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    Type:University open house

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  • 身のまわりの光を見てみよう!

    Role(s): Lecturer, Demonstrator

    国立研究開発法人科学技術振興機構(JST)  新宿SPP(Science Partnership Project)新宿区立西早稲田中学校2年生対象  2017.11    

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    Type:Visiting lecture

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  • Local organizing committee members

    Role(s): Organizing member

    公益社団法人日本生物工学会  第69回日本生物工学会大会  2017.9    

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    Type:Seminar, workshop

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  • 光る生物の解明をしよう!

    Role(s): Lecturer, Demonstrator

    国立研究開発法人科学技術振興機構(JST)  新宿SPP(Science Partnership Project)新宿区立牛込第一中学校2年生対象  2014.11    

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    Type:Visiting lecture

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  • Local organizing committee members

    Role(s): Organizing member

    公益社団法人日本水環境学会  Water and Environment Technology Conference 2014  2014.6    

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    Type:Seminar, workshop

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  • 夢を叶えるために,今必要なこと

    Role(s): Lecturer

    東京都青梅市立第二中学校  平成25年度第3学年進路講演会  2014.3    

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    Type:Lecture

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  • Local organizing committee members

    Role(s): Organizing member

    Nitrification Network  3rd International conference on nitrification  2013.9    

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    Type:Seminar, workshop

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  • 目には見えないミクロの世界をのぞいてみよう!―未知なる世界への挑戦―

    Role(s): Presenter, Lecturer, Organizing member, Demonstrator

    早稲田大学理工学術院  第23回ユニラブ(小中学生のための科学実験教室)  2010.8    

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    Type:Visiting lecture

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