2024/04/06 更新

写真a

ノガ アキラ
苗加 彰
NOGA Akira
所属
理工学部 助教C
連絡先
メールによる問い合わせは《こちら》から
外部リンク

学位

  • 博士(理学) ( 東京大学 )

  • 修士(理学) ( 東京大学 )

学歴

  • 2018年3月
     

    東京大学   理学系研究科   生物学専攻   博士   修了

  • 2014年3月
     

    東京大学   理学系研究科   生物学専攻   修士   修了

  • 2012年3月
     

    東京大学   理学部   生物学科   卒業

経歴

  • 2024年4月 - 現在

    中央大学   理工学部 生命科学科   助教

  • 2022年12月 - 2024年3月

    東京工業大学   科学技術創成研究院   特任助教

  • 2018年9月 - 2022年11月

    ポールシェラー研究所   ポスドク研究員

所属学協会

  • 2023年6月 - 現在

    日本生物物理学会

研究キーワード

  • クラミドモナス

  • クライオ電子線トモグラフィー

  • 繊毛

  • 中心子

研究分野

  • ライフサイエンス / 構造生物化学

  • ライフサイエンス / 細胞生物学

論文

  • ATP-induced conformational change of axonemal outer dynein arms revealed by cryo-electron tomography 査読

    Noemi Zimmermann, Akira Noga, Jagan Mohan Obbineni, Takashi Ishikawa

    EMBO JOURNAL   42 ( 12 )   2023年6月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)   出版者・発行元:WILEY  

    Axonemal outer dynein arm (ODA) motors generate force for ciliary beating. We analyzed three states of the ODA during the power stroke cycle using in situ cryo-electron tomography, subtomogram averaging, and classification. These states of force generation depict the prepower stroke, postpower stroke, and intermediate state conformations. Comparison of these conformations to published in vitro atomic structures of cytoplasmic dynein, ODA, and the Shulin-ODA complex revealed differences in the orientation and position of the dynein head. Our analysis shows that in the absence of ATP, all dynein linkers interact with the AAA3/AAA4 domains, indicating that interactions with the adjacent microtubule doublet B-tubule direct dynein orientation. For the prepower stroke conformation, there were changes in the tail that is anchored on the A-tubule. We built models starting with available high-resolution structures to generate a best-fitting model structure for the in situ pre- and postpower stroke ODA conformations, thereby showing that ODA in a complex with Shulin adopts a similar conformation as the active prepower stroke ODA in the axoneme.

    DOI: 10.15252/embj.2022112466

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  • Bld10p/Cep135 determines the number of triplets in the centriole independently of the cartwheel 査読

    Akira Noga, Mao Horii, Yumi Goto, Kiminori Toyooka, Takashi Ishikawa, Masafumi Hirono

    EMBO JOURNAL   41 ( 20 )   2022年10月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:WILEY  

    The conserved nine-fold structural symmetry of the centriole is thought to be generated by cooperation between two mechanisms, one dependent on and the other independent of the cartwheel, a sub-centriolar structure consisting of a hub and nine spokes. However, the molecular entity of the cartwheel-independent mechanism has not been elucidated. Here, using Chlamydomonas reinhardtii mutants, we show that Bld10p/Cep135, a conserved centriolar protein that connects cartwheel spokes and triplet microtubules, plays a central role in this mechanism. Using immunoelectron microscopy, we localized hemagglutinin epitopes attached to distinct regions of Bld10p along two lines that connect adjacent triplets. Consistently, conventional and cryo-electron microscopy identified crosslinking structures at the same positions. In centrioles formed in the absence of the cartwheel, truncated Bld10p was found to significantly reduce the inter-triplet distance and frequently form eight-microtubule centrioles. These results suggest that the newly identified crosslinks are comprised of part of Bld10p/Cep135. We propose that Bld10p determines the inter-triplet distance in the centriole and thereby regulates the number of triplets in a cartwheel-independent manner.

    DOI: 10.15252/embj.2020104582

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  • A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light 査読

    Osamu Kutomi, Ryosuke Yamamoto, Keiko Hirose, Katsutoshi Mizuno, Yuuhei Nakagiri, Hiroshi Imai, Akira Noga, Jagan Mohan Obbineni, Noemi Zimmermann, Masako Nakajima, Daisuke Shibata, Misa Shibata, Kogiku Shiba, Masaki Kita, Hideo Kigoshi, Yui Tanaka, Yuya Yamasaki, Yuma Asahina, Chihong Song, Mami Nomura, Mamoru Nomura, Ayako Nakajima, Mia Nakachi, Lixy Yamada, Shiori Nakazawa, Hitoshi Sawada, Kazuyoshi Murata, Kaoru Mitsuoka, Takashi Ishikawa, Ken-ichi Wakabayashi, Takahide Kon, Kazuo Inaba

    SCIENCE ADVANCES   7 ( 9 )   2021年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:AMER ASSOC ADVANCEMENT SCIENCE  

    Light-responsive regulation of ciliary motility is known to be conducted through modulation of dyneins, but the mechanism is not fully understood. Here, we report a novel subunit of the two-headed f/I1 inner arm dynein, named DYBLUP, in animal spermatozoa and a unicellular green alga. This subunit contains a BLUF (sensors of blue light using FAD) domain that appears to directly modulate dynein activity in response to light. DYBLUP (dynein-associated BLUF protein) mediates the connection between the f/I1 motor domain and the tether complex that links the motor to the doublet microtubule. Chlamydomonas lacking the DYBLUP ortholog shows both positive and negative phototaxis but becomes acclimated and attracted to high-intensity blue light. These results suggest a mechanism to avoid toxic strong light via direct photoregulation of dyneins.

    DOI: 10.1126/sciadv.abf3621

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  • Alternative evolution of a spheroidal colony in volvocine algae: developmental analysis of embryogenesis in Astrephomene (Volvocales, Chlorophyta) 査読

    Shota Yamashita, Yoko Arakaki, Hiroko Kawai-Toyooka, Akira Noga, Masafumi Hirono, Hisayoshi Nozaki

    BMC EVOLUTIONARY BIOLOGY   16   2016年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:BIOMED CENTRAL LTD  

    Background: Volvocine algae, which range from the unicellular Chlamydomonas to the multicellular Volvox with a germ-soma division of labor, are a model for the evolution of multicellularity. Within this group, the spheroidal colony might have evolved in two independent lineages: Volvocaceae and the goniacean Astrephomene. Astrephomene produces spheroidal colonies with posterior somatic cells. The feature that distinguishes Astrephomene from the volvocacean algae is lack of inversion during embryogenesis; the volvocacean embryo undergoes inversion after successive divisions to orient flagella toward the outside. The mechanisms of inversion at the molecular and cellular levels in volvocacean algae have been assessed in detail, particularly in Volvox carteri. However, embryogenesis in Astrephomene has not been subjected to such investigations.Results: This study relied on light microscopy time-lapse imaging using an actively growing culture of a newly established strain to conduct a developmental analysis of Astrephomene as well as to perform a comparison with the similar spheroidal volvocacean Eudorina. During the successive divisions involved in Astrephomene embryogenesis, gradual rotation of daughter protoplasts resulted in movement of their apical portions toward the embryonic posterior, forming a convex-to-spheroidal cell sheet with the apical ends of protoplasts on the outside. Differentiation of the posterior somatic cells from the embryo periphery was traced based on cell lineages during embryogenesis. In contrast, in Eudorina, the rotation of daughter protoplasts did not occur during successive cell divisions; however, inversion occurred after such divisions, and a spheroidal embryo was formed. Indirect immunofluorescence microscopy of basal bodies and nuclei verified this difference between Astrephomene and Eudorina in the movement of embryonic protoplasts.Conclusions: These results suggest different tactics for spheroidal colony formation between the two lineages: rotation of daughter protoplasts during successive cell divisions in Astrephomene, and inversion after cell divisions in Eudorina. This study will facilitate further research into the molecular and genetic mechanisms of the parallel evolution of the spheroidal colony in volvocine algae.

    DOI: 10.1186/s12862-016-0794-x

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  • SAS-6 engineering reveals interdependence between cartwheel and microtubules in determining centriole architecture 査読 国際共著

    Manuel Hilbert, Akira Noga, Daniel Frey, Virginie Hamel, Paul Guichard, Sebastian H. W. Kraatz, Moritz Pfreundschuh, Sarah Hosner, Isabelle Flueckiger, Rolf Jaussi, Mara M. Wieser, Katherine M. Thieltges, Xavier Deupi, Daniel J. Mueller, Richard A. Kammerer, Pierre Goenczy, Masafumi Hirono, Michel O. Steinmetz

    NATURE CELL BIOLOGY   18 ( 4 )   393 - +   2016年4月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:NATURE PUBLISHING GROUP  

    Centrioles are critical for the formation of centrosomes, cilia and flagella in eukaryotes. They are thought to assemble around a nine-fold symmetric cartwheel structure established by SAS-6 proteins. Here, we have engineered Chlamydomonas reinhardtii SAS-6-based oligomers with symmetries ranging from five- to ten-fold. Expression of a SAS-6 mutant that forms six-fold symmetric cartwheel structures in vitro resulted in cartwheels and centrioles with eight- or nine-fold symmetries in vivo. In combination with Bld10 mutants that weaken cartwheel-microtubule interactions, this SAS-6 mutant produced six- to eight-fold symmetric cartwheels. Concurrently, the microtubule wall maintained eight- and nine-fold symmetries. Expressing SAS-6 with analogous mutations in human cells resulted in nine-fold symmetric centrioles that exhibited impaired length and organization. Together, our data suggest that the self-assembly properties of SAS-6 instruct cartwheel symmetry, and lead us to propose a model in which the cartwheel and the microtubule wall assemble in an interdependent manner to establish the native architecture of centrioles.

    DOI: 10.1038/ncb3329

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  • Space-Dependent Formation of Central Pair Microtubules and Their Interactions with Radial Spokes 査読

    Yuki Nakazawa, Tetsuro Ariyoshi, Akira Noga, Ritsu Kamiya, Masafumi Hirono

    PLOS ONE   9 ( 10 )   2014年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Cilia and flagella contain nine outer doublet microtubules and a pair of central microtubules. The central pair of microtubules (CP) is important for cilia/flagella beating, as clearly shown by primary ciliary dyskinesia resulting from the loss of the CP. The CP is thought to regulate axonemal dyneins through interaction with radial spokes (RSs). However, the nature of the CP-RS interaction is poorly understood. Here we examine the appearance of CPs in the axonemes of a Chlamydomonas mutant, bld12, which produces axonemes with 8 to 11 outer-doublets. Most of its 8-doublet axonemes lack CPs. However, in the double mutant of bld12 and pf14, a mutant lacking the RS, most 8-doublet axonemes contain the CP. Thus formation of the CP apparently depends on the internal space limited by the outer doublets and RSs. In 10- or 11-doublet axonemes, only 3-5 RSs are attached to the CP and the doublet arrangement is distorted most likely because the RSs attached to the CP pull the outer doublets toward the axonemal center. The CP orientation in the axonemes varies in double mutants formed between bld12 and mutants lacking particular CP projections. The mutant bld12 thus provides the first direct and visual information about the CP-RS interaction, as well as about the mechanism of CP formation.

    DOI: 10.1371/journal.pone.0110513

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  • The Simplest Integrated Multicellular Organism Unveiled 査読

    Yoko Arakaki, Hiroko Kawai-Toyooka, Yuki Hamamura, Tetsuya Higashiyama, Akira Noga, Masafumi Hirono, Bradley J. S. C. Olson, Hisayoshi Nozaki

    PLOS ONE   8 ( 12 )   2013年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Volvocine green algae represent the "evolutionary time machine" model lineage for studying multicellularity, because they encompass the whole range of evolutionary transition of multicellularity from unicellular Chlamydomonas to >500-celled Volvox. Multicellular volvocalean species including Gonium pectorale and Volvox carteri generally have several common morphological features to survive as integrated multicellular organisms such as "rotational asymmetry of cells" so that the cells become components of the individual and "cytoplasmic bridges between protoplasts in developing embryos" to maintain the species-specific form of the multicellular individual before secretion of new extracellular matrix (ECM). However, these morphological features have not been studied in the four-celled colonial volvocine species Tetrabaena socialis that is positioned in the most basal lineage within the colonial or multicellular volvocine greens. Here we established synchronous cultures of T. socialis and carried out immunofluorescence microscopic and ultrastructural observations to elucidate these two morphological attributes. Based on immunofluorescence microscopy, four cells of the mature T. socialis colony were identical in morphology but had rotational asymmetry in arrangement of microtubular rootlets and separation of basal bodies like G. pectorale and V. carteri. Ultrastructural observations clearly confirmed the presence of cytoplasmic bridges between protoplasts in developing embryos of T. socialis even after the formation of new flagella in each daughter protoplast within the parental ECM. Therefore, these two morphological attributes might have evolved in the common four-celled ancestor of the colonial volvocine algae and contributed to the further increase in cell number and complexity of the multicellular individuals of this model lineage. T. socialis is one of the simplest integrated multicellular organisms in which four identical cells constitute the individual.

    DOI: 10.1371/journal.pone.0081641

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▼全件表示

講演・口頭発表等

  • Cryo-electron tomography identified novel filamentous structures composed of Bld10p/Cep135 that determine nine-fold symmetry of centriole 招待

    Akira Noga

    cryo-EM seminar  2022年11月 

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    記述言語:英語  

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  • 中心子の9回対称構造を規定するメカニズム -繊毛基底小体(中心子)の機能・構造解析- 招待

    苗加彰

    東工大化生研セミナー  2022年5月 

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    記述言語:日本語  

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  • Bld10p determines the centriole structure independently of the cartwheel

    Akira Noga, Takashi Ishikawa, Masafumi Hirono

    19th International Conference on the Cell and Molecular Biology of Chlamydomonas  2021年9月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • Cryo-electron tomography analysis of human centrioles

    Akira Noga, Roger Benoi, Loretta Müller, Takashi Ishikawa

    Cold Spring Harbor Asia Conference 2019 (Cilia and Centrosome)  2019年10月 

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    記述言語:英語   会議種別:ポスター発表  

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  • Structural difference between human and Chlamydmonas centrioles analyzed by Cryo-electron tomography

    Akira Noga, Roger Benoi, Loretta Müller, Masafumi Hirono, Takashi Ishikawa

    Interdisciplinary Symposium on 3D Microscopy  2019年10月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • Bld10p/Cep135 determines the number of triplets in the centriole independently of the cartwheel

    Akira Noga, Mao Horii, Masafumi Hirono

    5th International Volvox Conference  2019年7月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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  • Dynamic interaction between cartwheel and triplet microtubules establishes the nine-fold symmetry of the centriole.

    苗加彰

    2016年6月 

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    記述言語:英語   会議種別:口頭発表(一般)  

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受賞

  • 東京大学大学院理学系研究科研究奨励賞

    2014年3月   東京大学大学院理学系研究科  

  • 第12回東京大学生命科学シンポジウムポスター賞

    2012年6月   第12回 東京大学 生命科学シンポジウム  

  • 東京大学理学部学修奨励賞

    2012年3月   東京大学理学部  

共同研究・競争的資金等の研究課題

  • 緑藻クラミドモナスの2本の繊毛間の違いを生み出す分子機構の解明

    研究課題/領域番号:23K19346  2023年8月 - 2025年3月

    日本学術振興会  科学研究費助成事業  研究活動スタート支援  東京工業大学

    苗加 彰

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    配分額:2860000円 ( 直接経費:2200000円 、 間接経費:660000円 )

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  • 中心子の普遍的9回対称性構造の確立機構

    研究課題/領域番号:16J07088  2016年4月 - 2018年3月

    日本学術振興会  科学研究費助成事業  特別研究員奨励費  東京大学

    苗加 彰

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    配分額:1900000円 ( 直接経費:1900000円 )

    前年度から引き続き、中心子9回対称性構造の構築に働く、カートホイール非依存機構を担う実体を明らかにすることを目的とし、中心子タンパク質Bld10pの機能解析を行った。
    1.中心子におけるBld10pの局在
    前年度に樹立した、HAタグを付加したBld10p発現株を用いて免疫電子顕微鏡法を行い、私が発見した中心子微小管間の架橋構造の位置と比較した。その結果、Bld10pのN末端、分子中央、C末端ともに、カートホイールと三連微小管の結合部の間に一直線上に局在し、N末端の局在位置は、最も外周側の架橋構造と、分子中央とC末端の局在位置はそれよりも中心側の架橋構造とほとんど一致した。従って、これらの新規の架橋構造がBld10p分子によって形成されている可能性が高いと考えられる。
    2.カートホイール非存在下におけるBld10pの機能
    Bld10pが微小管間の架橋構造を構成し、それによりカートホイールに依存しない微小管集合に寄与しているのなら、Bld10pを改変して架橋を短くしたときに、中心子の9回対称性に影響を及ぼすはずである。そこで、カートホイールとBld10pを両方欠失する二重変異株bld10bld12に短いBld10pを発現させ、中心子構造に影響するかを検討した。前年度に樹立したbld10bld12に、短いBld10pを発現させ、それらの株の中心子構造を観察した。野生型Bld10pが発現している株では、微小管9本の中心子が最も多く観察され、7~11本に分布していたが、2種の発現株では、分布が微小管数の少ない方にシフトしており6~10本に分布し、微小管8本の中心子が最も多かった。さらに、架橋構造がbld12に比べて10%程度短くなっていた。これらのことから、短いBld10pの発現により、微小管間の距離が短くなり、中心子の対称性が小さい方にシフトしたと考えられる。

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メディア報道

  • 法政大など、細胞内小器官「中心子」が作られる仕組み解明−「水頭症」の治療に道 新聞・雑誌

    日刊工業新聞  2016年3月

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