Updated on 2024/12/07

写真a

 
YAMADA Taiga
 
Organization
Faculty of Science and Engineering Research Associate
Contact information
The inquiry by e-mail is 《here》
External link

Degree

  • 博士(工学) ( 中央大学 )

  • 修士(工学) ( 中央大学 )

Education

  • 2023.3
     

    Chuo University   doctor course   completed

  • 2018.3
     

    Chuo University   master course   completed

  • 2016.3
     

    Chuo University   graduated

Research History

  • 2024.4 - Now

    Chuo University   Faculty of Science and Engineering Department of Applied Chemistry   Assistant Professor

  • 2023.4 - 2024.3

    Chuo University   The Institute of Science and Engineering

Research Interests

  • ヘムタンパク質

  • 人工血液

  • バイオマテリアル

  • 光線力学療法

  • 抗がん剤

Papers

  • Hydrosulfide-methaemoglobin-albumin cluster: A hydrogen sulfide donor Reviewed

    Yuto Suzuki, Taiga Yamada, Yuki Enoki, Kazuaki Matsumoto, Teruyuki Komatsu, Kazuaki Taguchi

    Journal of Materials Chemistry B   12 ( 44 )   11515 - 11522   2024.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

    Methaemoglobin (metHb) possesses inherent characteristics that facilitate reversible binding to hydrogen sulfide. Exogenous hydrogen sulfide supplementation imparts beneficial bioactive effects, including antioxidant and anti-inflammatory; hence, we hypothesized that the metHb-hydrogen...

    DOI： 10.1039/d4tb01621a

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  • Self-Propelled Tubular Micromotors Powered by Hydrogen Bubbles under Mild Conditions: A Major Step toward Biological Applications with Live Cells Reviewed

    Mai Hashimoto, Yuma Sakai, Taiga Yamada, Ryo Kato, Teruyuki Komatsu

    ACS Applied Bio Materials   7 ( 11 )   7740 - 7747   2024.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acsabm.4c01350

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  • Synthesis and Physicochemical Properties of Poly(2‐Alkyl‐2‐Oxazoline)‐Conjugated Hemoglobins as an Artificial O2 Carrier: Comparison with Polyethylene Glycol Conjugates Reviewed

    Ryuhei Wakabayashi, Kota Ishii, Taiga Yamada, Teruyuki Komatsu

    Macromolecular Chemistry and Physics   2400183   2024.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Abstract

    Polyethylene glycol (PEG) is a biocompatible polymer extensively employed to prolong blood circulation and mitigate the immunogenicity of the protein therapeutics. Nonetheless, there are reports of anti‐PEG antibody production. Poly(2‐alkyl‐2‐oxazoline) (PROx) exhibits analogous immunological stealth properties and represents a potential substitute for PEG. This study details the synthesis of PROx‐conjugated hemoglobins (PROxm‐Hbs, R = Me, Et) as artificial O2 carriers, functioning as red blood cell (RBC) substitutes, and evaluates their O2 affinities and solution properties. The average number of polymers bound is designed to be 6 or 11. The O2 affinities (P50) of PMeOxm‐Hbs and PEtOxm‐Hbs are ascertained as 9 Torr, comparable to those of PEG‐conjugated Hbs (PEGm‐Hbs). The PEtOx6‐HbT, synthesized under an N2 atmosphere, exhibited a low O2 affinity (P50 = 57 Torr). Through the admixture of PEtOx6‐Hb and PEtOx6‐HbT, the P50 value of the formulation to range from 9 to 57 Tor can be tailored as desired. The colloid osmotic pressures and viscosities of PMeOxm‐Hb and PEtOxm‐Hb solutions are lower than those of the corresponding PEGm‐Hb solutions. Furthermore, the PROx conjugates evinced no cytotoxicity against normal cells. PMeOxm‐Hbs and PEtOxm‐Hbs, characterized by their appropriate O2 affinities and solution properties, present themselves as promising alternative materials to RBCs.

    DOI： 10.1002/macp.202400183

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  • Transport of Zinc‐Phthalocyanine to Cancer Cells Using Myoglobin−Albumin Fusion Protein for Photodynamic Therapy Invited Reviewed

    Taiga Yamada, Mizuki Funamoto, Ryoya Takada, Yoshitsugu Morita, Teruyuki Komatsu

    ChemBioChem   25 ( 17 )   e202400329   2024.9

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Abstract

    Photodynamic therapy (PDT) is a noninvasive approach to cancer treatment, wherein cell death is initiated by singlet oxygen (1O2) production via energy transfer from excited photosensitizers to ground‐state O2. Effective clinical photosensitizers necessitate water solubility for in vivo administration. Hydrophobic dyes, such as phthalocyanines, cannot be used directly as photosensitizers. Herein, we synthesized a myoglobin‐(human serum albumin) fusion protein reconstituted with zinc‐phthalocyanine (ZnPc), termed ZnPcMb‐HSA. The photophysical properties of ZnPcMb‐HSA closely resemble those of ZnPc‐substituted Mb. Notably, ZnPc dissociates from ZnPcMb‐HSA and selectively accumulates within cancer cells, while the protein components remain extracellular. Treatment of four distinct cell lines with ZnPcMb‐HSA, followed by red‐light irradiation, effectively induced apoptosis. The half‐maximal inhibitory concentrations (IC50) against these cancer cell lines ranged between 0.1–0.5 μM. Reconstituted Mb‐HSA emerges as a promising carrier for transporting various water‐insoluble porphyrinoid photosensitizer to target cancer cells in PDT applications.

    DOI： 10.1002/cbic.202400329

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  • Dual Delivery of Carbon Monoxide and Doxorubicin using Haemoglobin–Albumin Cluster: Proof of Concept for Well-Tolerated Cancer Therapy Reviewed

    Chihiro Ito, Kazuaki Taguchi, Taiga Yamada, Kengo Hanaya, Yuki Enoki, Takeshi Sugai, Teruyuki Komatsu, Kazuaki Matsumoto

    Journal of Materials Chemistry B   12 ( 23 )   5600 - 5608   2024.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

    The dual delivery of CO and DOX using HemoAct is a promising strategy as an anti-tumour agent to realise well-tolerated cancer therapy with minimal cardiotoxicity.

    DOI： 10.1039/d4tb00123k

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  • Zinc‐Substituted Hemoglobin–Albumin Cluster as a Porphyrin‐Carrier for Enhanced Photodynamic Therapy Reviewed

    Taiga Yamada, Maho Katsumi, Kota Ishii, Teruyuki Komatsu

    Chemistry – An Asian Journal   19 ( 11 )   e202400257   2024.6

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Abstract

    Apohemoprotein is focused on the field of theranostics, serving as a porphyrin carrier. Hemoglobin (Hb) consists of α2β2 tetramer with iron(II)‐protoporphyrin IX (heme) bound to each globin. However, heme‐removed Hb (apoHb) causes dissociation at αβ interfaces and aggregation under physiological conditions. We synthesized a stable apoHb derivative comprising intramolecular‐crosslinked apoHb (apoXHb) and human serum albumin (HSA), apoXHb‐HSA3. ApoXHb‐HSA3 engendered no aggregates in the physiological solutions. Moreover, apoXHb‐HSA3 was reconstituted with zinc(II)‐protoporphyrin IX (ZnP), generating ZnXHb‐HSA3, a potent photosensitizer for photodynamic therapy (PDT). The photophysical properties of ZnXHb‐HSA3 were identical to those of zinc‐substituted XHb (ZnXHb). Cellular uptake behavior was evaluated using various cancer cell lines. ZnXHb‐HSA3 released ZnP around the cells, and the free ZnP penetrated cell membranes. In contrast, protein units were not observed within the cells. ZnXHb‐HSA3 showed no cytotoxicity under dark conditions and demonstrated superior PDT activity in comparison to naked ZnXHb. ZnXHb‐HSA3 acts as an innovative porphyrin carrier for enhanced PDT.

    DOI： 10.1002/asia.202400257

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  • Polyoxazoline-Conjugated L-Asparaginase: An Antibody-Production-Free Therapeutic Agent for Acute Lymphoblastic Leukemia Reviewed

    Taiga Yamada, Marika Ishimaru, Takuma Shoji, Hirotaka Tomiyasu, Ryota Tochinai, Kazuaki Taguchi, Teruyuki Komatsu

    ACS Applied Bio Materials   6 ( 12 )   5789 - 5797   2023.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acsabm.3c00888

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  • Protein–Porphyrin Complex Photosensitizers for Anticancer and Antimicrobial Photodynamic Therapies Invited Reviewed

    Taiga Yamada, Teruyuki Komatsu

    ChemMedChem   18 ( 23 )   e202300373   2023.12

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Photodynamic therapy (PDT) efficiently induces apoptosis through visible‐light irradiation of photosensitizers (PSs) within tumors and microbial cells. Porphyrin analogues serve as widely utilized photosensitizing agents with their theranostic abilities being governed by molecular structures and central metal ions. However, these macrocyclic compounds tend to agglutinate and form stacks in aqueous environments, resulting in a loss of photochemical activity. To overcome this limitation, encapsulation within liposomes and polymer micelles enables the dispersion of porphyrins as monomolecular entities in aqueous solutions, preventing undesirable deactivation. Recently, the use reconstituted hemoproteins containing various metal‐porphyrins and protein cages incorporating porphyrins have garnered significant interest as a new generation of biocompatible PSs. In this concept paper, we provide a comprehensive review of recent developments and trends of protein–porphyrin complex PSs for applications in anticancer and antimicrobial PDTs.

    DOI： 10.1002/cmdc.202300373

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  • Zinc Substituted Myoglobin−Albumin Fusion Protein: A Photosensitizer for Cancer Therapy Reviewed

    Taiga Yamada, Yoshitsugu Morita, Ryoya Takada, Mizuki Funamoto, Wataru Okamoto, Mitsutomo Kohno, Teruyuki Komatsu

    Chemistry – A European Journal   29 ( 22 )   e202203952   2023.4

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Abstract

    Myoglobin combined with human serum albumin (Mb‐HSA) can be produced using yeast Pichia pastoris as a host strain, with secretion into the culture medium. This Mb‐HSA fusion protein possesses identical O2 binding affinity to that of naked Mb. The Mb unit is reconstituted with a zinc(II) protoporphyrin IX, yielding (zinc substituted Mb)‐HSA, ZnMb‐HSA. The photophysical property and singlet O2 generation ability of ZnMb‐HSA are equivalent to those of ZnMb. In vitro cell experiments revealed that ZnMb‐HSA acts as a superior photosensitizer for photodynamic cancer therapy. It is noteworthy that ZnMb‐HSA shows long circulation lifetime in vivo.

    DOI： 10.1002/chem.202203952

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/chem.202203952

  • Catalase–albumin cluster incorporating protoporphyrin IX: an O2 generating photosensitizer for enhanced photodynamic therapy Reviewed

    Taiga Yamada, Maho Katsumi, Yuka Yagisawa, Masato Ichihara, Teruyuki Komatsu

    Mater. Adv.   3 ( 16 )   6451 - 6457   2022.8

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

    Catalase enwrapped covalently with human serum albumin incorporating protoporphyrin IX is a unique dual functional hemoprotein cluster (O2 generation and photosensitization) that can achieve enhanced photodynamic therapy.

    DOI： 10.1039/d2ma00242f

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  • Methemoglobin‐Albumin Cluster Incorporating Protoporphyrin IX: Dual Functional Protein Drug for Photodynamic Therapy Reviewed

    Taiga Yamada, Teruyuki Komatsu

    ChemBioChem   22 ( 15 )   2526 - 2529   2021.8

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/cbic.202100213

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    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/cbic.202100213

  • Quaternary Structure Analysis of a Hemoglobin Core in Hemoglobin–Albumin Cluster Reviewed

    Yoshitsugu Morita, Taiga Yamada, Moeka Kureishi, Kiyohito Kihira, Teruyuki Komatsu

    J. Phys. Chem. B   122 ( 50 )   12031 - 12039   2018.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acs.jpcb.8b10077

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  • Structural Insights into a Hemoglobin–Albumin Cluster in Aqueous Medium Reviewed

    Ryuichi Shinohara, Taiga Yamada, Boris Schade, Christoph Böttcher, Takaaki Sato, Natsuhiko Sugimura, Toshimichi Shibue, Teruyuki Komatsu

    J. Phys. Chem. Lett.   8 ( 4 )   819 - 824   2017.2

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acs.jpclett.6b02907

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Presentations

  • 亜鉛置換HemoActの合成と光線⼒学療法への応⽤

    山田大雅, 小松晃之

    第 31 回⽇本⾎液代替物学会年次⼤会  2024.12 

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  • ポリオキサゾリン結合ウリカーゼの合成と酵素活性評価

    庄司 拓真, 山田 大雅, 小松 晃之

    日本化学会秋季事業 第14回 CSJ化学フェスタ2024  2024.10 

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  • ビオチン化アルブミンの有無を識別できる高分子チューブ マイクロモーターの合成

    木村 光里, 山田 大雅, 小松晃之

    第73回高分子討論会  2024.9 

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  • Synthesis and photodynamic activity of zinc-phthalocyanine substituted myoglobin-albumin fusion protein

    YAMADA Taiga, KOMATSU Teruyuki

    2024.9 

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    Event date： 2024.9 　 　

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • 亜鉛フタロシアニン置換ミオグロビン－アルブミン融合タンパク質の合成と光殺菌能

    江川 碧, 舩本 瑞稀, 山田 大雅, 小松 晃之

    日本化学会第104春季年会  2024.3 

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  • 高分子結合アスパラギナーゼの合成と抗がん活性

    庄司拓真, 山田大雅, 小松晃之

    第72回高分子討論会  2023.9 

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    Language：Japanese  

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  • Hemoprotein-Albumin clusters Incorporating Protoporphyrin IX for Enhanced Photodynamic Therapy

    2023.9 

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  • (亜鉛置換ヘモグロビン-アルブミン)クラスターの合成と光線力学活性

    勝見 真帆, 山田 大雅, 小松 晃之

    錯体化学会 第73回討論会  2023.9 

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  • 亜鉛フタロシアニン置換ミオグロビン‐アルブミン融合タンパク質の合成と抗がん活性

    舩本 瑞稀, 山田 大雅, 小松 晃之

    錯体化学会 第73回討論会  2023.9 

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  • ポリ(2-エチル-2-オキサゾリン)結合アスパラギナーゼの合成と細胞毒性

    庄司 拓真, 石丸 真里花, 山田 大雅, 小松 晃之

    日本化学会第103春季年会  2023.3 

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  • プロトポルフィリン結合HemoAct製剤の合成と光線力学療法への応用

    山田大雅, 勝見真帆, 小松晃之

    第29回日本血液代替物学会年次大会  2022.12 

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  • (亜鉛置換ヘモグロビン－アルブミン)クラスターの合成

    石井幸太, 勝見真帆, 山田大雅, 小松晃之

    第12回CSJ化学フェスタ2022  2022.10 

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  • (亜鉛ヘモグロビン－アルブミン)クラスターの合成と光線力学活性

    勝見真帆, 山田大雅, 小松晃之

    錯体化学会第72回討論会  2022.9 

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  • がん治療光増感剤としてのプロトポルフィリン IX 結合(カタラーゼ－アルブミン)クラスターの合成

    山田大雅, 勝見真帆, 小松晃之

    錯体化学会第72回討論会  2022.9 

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  • 白血病治療薬としてのポリオキサゾリン修飾L-アスパラギナーゼの合成と抗がん活性

    石丸真里花, 山田大雅, 小松晃之

    第71回高分子討論会  2022.9 

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  • プロトポルフィリン結合(カタラーゼ－アルブミン)クラスターの光線力学活性

    山田大雅, 勝見真帆, 小松晃之

    第71回高分子学会年次大会  2022.5 

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  • プロトポルフィリンIX結合(カタラーゼ－アルブミン)クラスターの合成と光線力学活性

    勝見真帆, 山田大雅, 小松晃之

    日本化学会第102春季年会  2022.3 

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  • O2-Affinity Control and Structure Analysis of Hemoglobin-Albumin Clusters

    T. Yamada, Y. Morita, T. Komatsu

    The 2021 International Chemical Congress of Pacific Basin Societies (Pacifichem)  2021.12 

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  • 抗酸化剤を結合した(ヘモグロビン－アルブミン)クラスターの合成

    石丸真里花, 山田大雅, 小松晃之

    第15回バイオ関連化学シンポジウム  2021.9 

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  • 抗酸化能を有する(ヘモグロビン－アルブミン)クラスターの合成

    石丸真里花, 山田大雅, 小松晃之

    第33回生物無機化学夏季セミナー  2021.7 

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  • がん治療用光増感剤としてのプロトポルフィリンⅨ結合(ヘモグロビン－アルブミン)クラスターの合成

    山田大雅, 小松晃之

    第70回高分子学会年次大会  2021.5 

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  • Modulation of O2-Binding Affinity of Hemoglobin-Albumin Cluster as an Artificial O2 Carrier

    Y. Morita, T. Yamada, T. Komatsu

    43rd International Conference on Coordination Chemistry (ICCC 2018)  2018.8 

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  • Structure and Oxygen Binding Affinity of Hemoglobin-Albumin Cluster as an Artificial Oxygen Carrier

    Y. Morita, T. Yamada, T. Komatsu

    日本化学会第98春季年会  2018.3 

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  • 低酸素親和性(ヘモグロビン－アルブミン)クラスターの構造

    山田大雅, 森田能次, 小松晃之

    第７回CSJ化学フェスタ2017  2017.10 

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  • 低酸素親和性(ヘモグロビン－アルブミン)クラスターの合成と構造

    山田大雅, 森田能次, 小松晃之

    錯体化学会第67回討論会  2017.9 

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  • アルブミン結合数の異なる(ヘモグロビン－アルブミン)クラスターの構造と酸素親和性

    鹿島知周, 山田大雅, 森田能次, 小松晃之

    錯体化学会第67回討論会  2017.9 

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  • アルブミン結合数の異なる(ヘモグロビン－アルブミン)クラスターの単離と酸素結合能

    鹿島知周, 山田大雅, 船木亮佑, 小松晃之

    日本化学会第97春季年会  2017.3 

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  • (ヘモグロビン－アルブミン)クラスターの酸素結合能と構造解析

    船木亮佑, 山田大雅, C. Böttcher, 小松晃之

    第６回CSJ化学フェスタ2016  2016.11 

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  • 3D-Structure and O2-Binding Property of Hemoglobin-Albumin Cluster

    R. Funaki, T. Yamada, C. Böttcher, T. Komatsu

    IUPAC 12th International Conference on Novel Materials and their Synthesis (NMS-XII)  2016.10 

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  • (ヘモグロビン－アルブミン)クラスターの酸素親和性制御

    山田大雅, 小松晃之

    錯体化学会第66回討論会  2016.9 

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  • (ヘモグロビン－ウシ血清アルブミン)クラスターの合成と酸素結合能

    山田大雅, 篠原隆一, 小松晃之

    日本化学会第96春季年会  2016.3 

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Awards

  • 第36回渋谷健一奨励賞

    2023.3   中央大学  

  • 最優秀学生講演賞

    2022.12   日本血液代替物学会  

  • 第35回渋谷健一奨励賞

    2022.3   中央大学  

  • 高分子学会優秀ポスター賞

    2021.5   高分子学会