記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1007/s10544-020-00517-0

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掲載種別：研究論文（国際会議プロシーディングス）  

© 17CBMS-0001. This paper describes a chick embryo cardiomyocyte actuator for investigation of cardiomyocyte contractile properties as alternatives of culture system with rodent cardiomyocytes. Chick embryo cardiomyocytes are more accessible than rat or mouse cardiomyocytes in terms of cost and ethics. We cultured chick embryo cardiomyocytes on parylene sheets and observed that the parylene sheets autonomously flapped with the contractile force of cardiomyocytes. Furthermore, the flap frequency increased in response to the addition of isoproterenol, which promotes contraction of cardiac muscles. This technique would reduce the cost of experiments, and thus be expected to promote the study of contractile properties of cardiomyocytes.

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掲載種別：研究論文（国際会議プロシーディングス）  

We propose a hydrogel-based transparent subdural electrode with ionic connection for intraoperative recording brain waves (electrocorticography, ECoG). The present electrode was composed of a poly(dimethylsiloxane) (PDMS) microchannel filled with ionically conductive hydrogel (salt bridge) was embedded to a polyvinyl alcohol (PVA) hydrogel as substrate. The salt bridge acts as an ionic connection between the electrochemical interface and brain surface. The fabricated soft electrode showed high conformability to the curved wet surface of brains with optical transparency, which is an advantage for a subdural electrode. In vivo recording of porcine brain waves proved that the salt bridge electrode could be practical.

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掲載種別：研究論文（国際会議プロシーディングス）  

The monitoring of bio-information through skin with wearable devices has been attracted as an advanced mean for daily health management. We developed a patch-type wearable device composed of a novel porous microneedle (PMN) and a flexible, stable Ag/AgCl reference electrodes. The whole of PMN contains 3D microchannels, and serves as a minimally invasive interface for the transdermal transport of molecules and ions. By using the flexible PMN patch, we realized the monitoring of the transepidermal potential (TEP), a potential difference generated along the thickness direction of the epidermis, which is an useful indicator of skin health status.

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1021/acsaelm.9b00671

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/admt.201900889

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

A totally soft organic subdural electrode has been developed by embedding an array of poly(3,4-ethylenedioxythiophene)-modified carbon fabric (PEDOT-CF) into the polyvinyl alcohol (PVA) hydrogel substrate. The mesh structure of the stretchable PEDOT-CF allowed stable structural integration with the PVA substrate. The electrode performance for monitoring electrocorticography (ECoG) was evaluated in saline solution, on ex vivo brains, and in vivo animal experiments using rats and porcines. It was demonstrated that the large double-layer capacitance of the PEDOT-CF brings low impedance at the frequency of brain wave including epileptic seizures, and PVA hydrogel substrate minimized the contact impedance on the brain. The most important unique feature of the hydrogel-based ECoG electrode was its shape conformability to enable tight adhesion even to curved, grooved surface of brains by just being placed. In addition, since the hydrogel-based electrode is totally organic, the simultaneous ECoG-fMRI measurements could be conducted without image artifacts, avoiding problems induced by conventional metallic electrodes.

DOI： 10.1038/s41598-019-49772-z

PubMed

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1371/journal.pone.0219198

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1115/1.4043522

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/mabi.201900060

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

This paper addresses a nanoengineering approach to create a fully three-dimensional (3D) network of living cells, providing an advanced solution to in vitro studies on either neuronal networks or artificial organs. The concept of our work relies on stackable scaffolds composed of microcontainers designed and dimensioned to favor the geometrically constrained growth of cells. The container geometry allows cells to communicate in the culture medium and freely grow their projections to form a 3D arrangement of living cells. Scaffolds are fabricated using two-photon polymerization of IP-L 780 photoresist and are coated with collagen. They are stacked by mechanical micromanipulation. Technical details of the proposed nanofabrication scheme and assembly of the modular culture environment are explained. Preliminary in vitro results using PC12 cells have shown that this structure provides a good basis for healthy cell growth for at least 16 days. Our approach is envisioned to provide tailor-made solutions of future 3D cell assemblies for potential applications in drug screening or creating artificial organs.

DOI： 10.1039/c8lc01240g

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.bios.2018.07.013

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MARY ANN LIEBERT, INC  

We propose a pneumatic bending actuator integrated with a low-melting-point alloy-based variable stiffness endoskeleton that can bend at multiple points and maintain its bent shape without power supply. Local stiffness of the soft actuator can be altered by melting or hardening the endoskeleton with electric heat applied through embedded metal wires. Bending points of the actuator can be changed by selecting different points of the endoskeleton to be melted, and the bending angle can be controlled by injected air pressure. The shape of the bent actuator is maintained by hardening the alloy even when pressure is reduced to the initial state. We demonstrate that the actuator can be bent differently with only one pneumatic actuation layer by combining multipoint bending and the shape retention function, and thus the actuator can be used for lifting, holding, and unloading an object. We believe that the simple machinery of the actuator will be useful in programming complicated motions of soft robotic fingers, fins, and tentacles.

DOI： 10.1089/soro.2017.0145

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：MDPI  

Engineering of neuronal network geometry by micropatterning technology is a key future technology for creating artificial brains on a chip. However, engineering of network geometry at the single-cell-level with functional morphology (axon/dendrite) and connectivity (synapses) is still challenging. Here, we describe a method for controlling the axon and dendrite morphology of single primary-cultured neurons and assembling a neural circuit using mobile microplates. The microplates enabled morphological control of neurons by their shapes and bringing their ends into contact caused the formation of physical connections. Functional synapse formation at the connection was indicated by immunostaining of synapse-related proteins and intracellular Ca2+ imaging of neural activity. We believe that the method will be useful in engineering neural circuits with selected neurons and defined morphology.

DOI： 10.3390/mi9050235

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記述言語：英語  

Using traditional 2-D photolithographic methods, surface patterns are made on agarose and used to form lipid vesicles with controlled size and layout. Depending on the size and layout of the patterned structures, the lipid bilayer vesicle size can be tuned and placement can be predetermined. Vesicles formed on 2-D patterned surfaces can be harvested for further investigations or can be assayed directly on the patterned surface. Lipid vesicles on the patterned surface are assayed for unilamellarity and protein incorporation, and vesicles are indeed unilamellar as observed from outer leaflet fluorescence quenching. Vesicles successfully incorporate the integral membrane protein α-hemolysin and maintain its membrane transport function.

DOI： 10.1063/1.5002604

PubMed

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掲載種別：研究論文（国際会議プロシーディングス）  

We propose a hydrogel-based bioreactor integrated with organic electrodes for efficient electrical stimulation of cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs). The bioreactor was fabricated by integration of poly(3,4-ethylenedioxythiophene) (PEDOT)/polyurethane (PU) electrodes and a hydrogel with a cell-positioning chamber. The fabricated organic electrodes had a high capacity compared to conventional metal electrodes that prevented unfavorable electric polarization. Cardiomyocytes derived from hiPSCs were cultured in the bioreactor, and their beating frequency was synchronized to frequency of electrical stimulation applied by the electrodes. Efficient electrical stimulation was achieved by optimizing configuration of the electrodes.

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1088/0960-1317/26/9/095017

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掲載種別：研究論文（学術雑誌）  

DOI： 10.1002/adhm.201500782

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：WILEY-V C H VERLAG GMBH  

DOI： 10.1002/smll.201502968

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：IOP PUBLISHING LTD  

This paper describes a liquid-filled tunable lenticular lens for switching between two-dimensional (2D) and three-dimensional (3D) images in naked-eye 3D displays. Compared with previous 2D/3D switchable displays, this tunable lenticular lens that is directly attached to a smartphone display can project both a 2D image with the original resolution of the smartphone display and a 3D image with high brightness. This lens is simply composed of transparent poly(dimethylsiloxane) (PDMS) microchannels. While the thin top membrane on the microchannels is normally flat to transmit light without deflection for displaying 2D images, applying pressure to the microchannel deforms the membrane to acquire characteristics of lenticular lenses for 3D images. We successfully demonstrate the switching between the 2D and 3D modes. We believe that our lens can be applied as a part of a portable 2D/3D naked-eye 3D display.

DOI： 10.1088/0960-1317/25/3/035030

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DOI： 10.20965/ijat.2014.p0095

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：SPRINGER  

We compare the generalization characteristics of complex-valued and real-valued feedforward neural networks. We assume a task of function approximation with phase shift and/or amplitude change in signals having various coherence. Experiments demonstrate that complex-valued neural networks show smaller generalization error than real-valued networks having doubled input and output neurons in particular when the signals have high coherence, that is, high degree of wave nature. We also investigate the relationship between amplitude and phase errors. It is found in real-valued networks that abrupt change in amplitude is often accompanied by steep change in phase, which is a consequence of local minima in real-valued supervised learning.

DOI： 10.1007/s00521-012-0960-z

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掲載種別：研究論文（国際会議プロシーディングス）  

This paper describes mobile microplates for morphological control and handling of single neural cells. We fabricated mobile microplates having a center circle (termed "Base") and radiated lines (termed "Path") for controlling the shape of neural cells: cell bodies and neurites. Single neural cells were cultured on the microplates, and their morphologies were fully controlled by the Base/Path structures. The morphologically controlled single neural cells on the microplates were also three-dimensionally handlable by microtweezers. We believe our method would be useful in the assembly of cultured neuronal networks at the single cell level.

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC  

Applications of complex-valued neural networks (CVNNs) have expanded widely in recent years-in particular in radar and coherent imaging systems. In general, the most important merit of neural networks lies in their generalization ability. This paper compares the generalization characteristics of complex-valued and real-valued feedforward neural networks in terms of the coherence of the signals to be dealt with. We assume a task of function approximation such as interpolation of temporal signals. Simulation and real-world experiments demonstrate that CVNNs with amplitude-phase-type activation function show smaller generalization error than real-valued networks, such as bivariate and dual-univariate real-valued neural networks. Based on the results, we discuss how the generalization characteristics are influenced by the coherence of the signals depending on the degree of freedom in the learning and on the circularity in neural dynamics.

DOI： 10.1109/TNNLS.2012.2183613

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掲載種別：研究論文（国際会議プロシーディングス）  

This paper describes a method to assemble three-dimensional (3D) microstructures by gluing separated two-dimensional (2D) structures attaching with living cells at micron-scales using hydrogels. We utilized photo-curable, biocompatible poly(ethylene)-glycol-diacrylate (PEGDA) as a glue, and assembled 2D cell-laden microplates into 3D structures by photoassisted local polymerizations. We demonstrated an assembly of 3D half-cubic microstructures with genetically-labeled cells, which enabled observing intracellular microstructure in 3D cellular constructs. We believe our method is useful for assembling 3D structures with targeted cells and into arbitrary geometries.

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記述言語：英語   出版者・発行元：一般社団法人 日本生物物理学会  

DOI： 10.2142/biophys.51.S127_1

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掲載種別：研究論文（国際会議プロシーディングス）  

This paper describes a method to assemble single neural cells into neural networks by utilizing microfabricated mobile microplates. Single neural cells were cultured on the mobile microplates. Directions of their neurite growth were controlled by guidepaths attached beside the microplates. We demonstrated that single neural cells with directed neurites were moved and assembled by mobile microplates into desired network structure, and also the network geometry was redefined. We believe this modular assembly method could be applied to precise patterning of neural networks, and also useful in basic studies of single-cell-level neural networks.

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記述言語：英語   著書種別：学術書

This paper introduces a new technique for patterning functionalization layers on substrates with hightopogra phy. The method is based on a parylene-C template shaped by a structured, sacrificial photoresist layer and attached to the substrate where functionalization is not intended. After photoresist removal and surface function ali za tion, the parylene layer is peeled off, leaving all areas initially covered by the sacrificial polymer functionalized. The technique has several advantages: （i） In contrast to microcontact printing, it allows surfaces with complex topographies to be functionalized; （ii） complex function al ization patterns are possible; （iii） the parylene structure can be reutilized. We successfully demonstrate the technique with the guided growth of neuron-like PC12 cells on honeycomb-shaped protein patterns on micro pillars and microwells. The range and limits of the technique are analyzed and discussed in detail.

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We propose a fluorescence detection system for a portable odorant biosensor composed of cell spheroids with expressed olfactory receptors and a CMOS chip. As fluorescence intensity of spheroids is higher than that of single cells, we achieved detection of fluorescence signals caused by intracellular chemical reaction on the CMOS chip. As a demonstration of odorant detection, we prepared spheroids of cells expressing olfactory receptors to certain odorant molecules. The CMOS chip could detect the increment of the fluorescence intensity around the spheroids when odorant molecules were applied. We believe that the system will contribute to establish portable odorant biosensors.

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記述言語：英語   出版者・発行元：IEEE  

This paper reports a system enabling cell detection, fluorescence light detection and single cell retrieval. It consists of a device combining a microplate technology, allowing single cell seeding on individual cell-sized paiylene plates, and a CMOS imaging sensor placed on the right bottom of the microplate chip with glass substrate. By using such a system, we succeeded to visualize individual cells, to detect fluorescence light emission and to retrieve a single cell by using a micromanipulator. We believe that the proposed system will be useful for the point of care analysis on the level of single cell analysis.

DOI： 10.1109/MEMSYS.2016.7421668

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記述言語：英語   出版者・発行元：Institute of Industrial Science The University of Tokyo  

This paper introduces a new technique for patterning functionalization layers on substrates with hightopogra phy. The method is based on a parylene-C template shaped by a structured, sacrificial photoresist layer and attached to the substrate where functionalization is not intended. After photoresist removal and surface function ali za tion, the parylene layer is peeled off, leaving all areas initially covered by the sacrificial polymer functionalized. The technique has several advantages: （i） In contrast to microcontact printing, it allows surfaces with complex topographies to be functionalized; （ii） complex function al ization patterns are possible; （iii） the parylene structure can be reutilized. We successfully demonstrate the technique with the guided growth of neuron-like PC12 cells on honeycomb-shaped protein patterns on micro pillars and microwells. The range and limits of the technique are analyzed and discussed in detail.

DOI： 10.11188/seisankenkyu.67.521

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J-GLOBAL

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記述言語：英語   出版者・発行元：IEEE  

We report a method to measure contractile forces of cardiomyocytes at cellular level using microplates; pairs of single-cell sized plates are connected by a flexible hinge at the center. Cardiomyocytes repeatedly contract and relax, and thereby fold and stretch the microplates at the flexible hinge. By measuring the change of the bending angle in folded microplates, we can estimate contractile forces of cardiomyocytes. We believe that this method is a useful tool to study the biomechanics of cardiomyocytes.

DOI： 10.1109/MEMSYS.2015.7051024

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記述言語：日本語   出版者・発行元：Institute of Electrical Engineers of Japan  

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記述言語：日本語   出版者・発行元：Institute of Electrical Engineers of Japan  

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記述言語：日本語   出版者・発行元：Institute of Electrical Engineers of Japan  

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© 14CBMS. We describe a centrifuge-based dynamic microarray system for trapping an array of single cells from small amount of suspension. We optimized microchannel designs and centrifugal force for minimizing sample volumes and maximizing trapping efficiencies, and achieved single-cell trapping by introducing only 5 μl of cell suspension with the centrifugal force of 70 G. Trapping of cells stained with DAPI shows that single cells were trapped at desired trapping spots. This system will be applicable for single cell assays using rare or expensive cells.

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記述言語：日本語   出版者・発行元：Institute of Electrical Engineers of Japan  

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記述言語：英語   出版者・発行元：Institute of Electrical Engineers of Japan  

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記述言語：日本語   出版者・発行元：一般社団法人日本機械学会  

We achieved three-dimensional (3D) fabrication of heterogeneous microstructures using an in situ photo-polymerization method, vertical continuous flow lithography (VCFL). Utilizing a projection system including a Digital Mirror Device, 3D microstructures were fabricated in a layer-by-layer fashion with the thickness of each layer controlled by UV absorber and exposure time. In addition, VCFL fabricated heterogeneous microstructures by exposing light patterns to multiple laminar flows. We demonstrated three-layered structures and long fibers whose cross-sectional geometries were changed. We also fabricated two-layered Janus structures. We believe that VCFL will be applicable to making anisotropic microparticles for material science and bottom up tissue engineering.

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記述言語：英語  

This paper describes a tunable lenticular lens to switch high-resolution two-dimensional (2D) / three-dimensional (3D) images in naked-eye stereoscopic displays. This lens is simply composed of transparent poly(dimethylsiloxane) (PDMS) microchannels and pumps to realizes two states: (A) flat surface for light transmissions without deflection
(B) cylindrically deformed surface for light deflection. This lens has three advantages: (i) switchable optical characteristics without patterned electrode, (ii) adjustable focal length by the pressure-driven deformation, (iii) simple fabrication and low-cost experimental setup. By using this device put on a Smart Phone display, we successfully observed 3D images and two different images depending on view angle. Moreover, we obtained clear 2D images without decrease in the horizontal resolution. We believe that this device can be applied to a portable and high luminance naked-eye stereoscopic display by the combination with various types of commercially available portable displays. © 2013 IEEE.

DOI： 10.1109/Transducers.2013.6627135

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記述言語：日本語   出版者・発行元：一般社団法人電子情報通信学会  

本論文は、複素および実数フィードフォワード・ニューラルネットワークの汎化特性を、特に扱われる信号のコヒーレンスの変化に対してどのように変わるか実験的に比較し、その結果を考察する。ここでは、フィルタリングなどの関数近似をタスクとする。シミュレーションの結果、振幅-位相型の活性化関数を持つ複素ニューラルネットワークは、良い汎化特性を示すことが示される。また双単変数の実数ニューラルネットワークも比較的良い汎化特性を示す。その他にも、2変数実数ニューラルネットワーク、実部虚部分離型活性化関数による汎化特性も調査した。その結果をもとに、信号の円環性とコヒーレンスが、それぞれのニューラルネットワークの汎化特性にどのように影響を与えるかを考察した。

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記述言語：英語   出版者・発行元：SPRINGER-VERLAG BERLIN  

We compare the generalization characteristics of complex-valued and real-valued feedforward neural networks when they deal with wave-related signals. We assume a task of function approximation. Experiments demonstrate that complex-valued neural networks show smaller generalization error than real-valued ones in particular when the signals have high degree of wave nature.

DOI： 10.1007/978-3-642-24955-6_63

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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記述言語：日本語   会議種別：ポスター発表   国名：日本  

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会議種別：口頭発表（一般）  

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会議種別：ポスター発表  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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会議種別：口頭発表（招待・特別）  

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開催年月日： 2020年 　 　

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開催年月日： 2020年 　 　

記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（招待・特別）  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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開催年月日： 2019年10月 　 　

記述言語：英語   会議種別：口頭発表（一般）  

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開催年月日： 2019年10月 　 　

記述言語：英語   会議種別：ポスター発表  

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開催年月日： 2019年10月 　 　

記述言語：英語   会議種別：ポスター発表  

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開催年月日： 2019年10月 　 　

記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2018年12月 　 　

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2018年12月 　 　

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2018年12月 　 　

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2018年11月 　 　

記述言語：日本語   会議種別：ポスター発表  

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開催年月日： 2018年11月 　 　

記述言語：日本語   会議種別：ポスター発表  

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開催年月日： 2018年11月 　 　

記述言語：英語   会議種別：ポスター発表  

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開催年月日： 2018年9月 　 　

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2018年9月 　 　

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2018年9月 　 　

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2018年9月 　 　

記述言語：日本語   会議種別：口頭発表（一般）  

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We propose a bioactuator driven by a chick embryo cardiomyocyte for the investigation of cardiomyocytes as alternatives of rodent cardiomyocytes driven actuator. Chick embryo cardiomyocytes are more accessible than rodent cardiomyocytes in terms of cost and ethics. Here, we cultured chick embryo cardiomyocytes on parylene films and observed that the edges of parylene films were autonomously lifted up and down by the contractile force of cardiomyocytes. Moreover, the frequency of flapping increased in response to the addition of isoproterenol, which promotes contraction of cardiac muscles. This bioactuator would reduce the cost of experiments and thus be expected to promote the study of contractile properties of cardiomyocytes.

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In this paper, we report a microfluidic device for pairing uniformly sized liposomes and fusing the paired liposomes. Our microfluidic device is composed of dynamic micro array for pairing liposomes and three-dimensional electrode for fusing liposomes. Electrodes should be located in proper position of a dynamic micro array for applying sufficient electric field to fuse liposomes. By using low melting point alloy, 3D electrodes were integrated in the dynamic microarray device to fuse the paired liposomes. Two uniformly sized liposomes were successfully paired in the dynamic micro array device and fused by the integrated 3D electrode. We believe that this dynamic micro array device will become a useful microfluidics device for vesicle fusion.

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This manuscript describes selective electrofusion of cell-sized liposomes using microfabricated dielectrophoretic tweezer. We fabricated round-tip dielectrophoretic tweezers by microfabrication techniques. We enabled selective manipulation of liposomes by applying alternate current to the tweezer. As we control the frequency of the current, we attract or repulse the liposomes. Liposomes were selectively manipulated one-by-one and paired by the tweezers. Finally we paired two cell-sized liposomes in a hand-made electrofusion chamber by using the tweezer. The paired liposomes were contact with each other by applying alternate current, then fused by pulses of direct current. We believe our technique will be useful in liposome and cell fusion study for artificial cell research.

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記述言語：日本語  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：英語  

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記述言語：日本語  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：日本語  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：日本語  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：日本語  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：ポスター発表  

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記述言語：日本語  

本論文は、複素および実数フィードフォワード・ニューラルネットワークの汎化特性を、特に扱われる信号のコヒーレンスの変化に対してどのように変わるか実験的に比較し、その結果を考察する。ここでは、フィルタリングなどの関数近似をタスクとする。シミュレーションの結果、振幅-位相型の活性化関数を持つ複素ニューラルネットワークは、良い汎化特性を示すことが示される。また双単変数の実数ニューラルネットワークも比較的良い汎化特性を示す。その他にも、2変数実数ニューラルネットワーク、実部虚部分離型活性化関数による汎化特性も調査した。その結果をもとに、信号の円環性とコヒーレンスが、それぞれのニューラルネットワークの汎化特性にどのように影響を与えるかを考察した。

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記述言語：英語   会議種別：ポスター発表  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語  

ニューラルネットワークの最も特徴的で重要な機能の一つは、汎化である。本稿は、複素ニューラルネットワークと実数ニューラルネットワークの汎化特性を比較する。特に、複素ニューラルネットワークが波動的なデータを扱う際の汎化特性が、信号の波動性によってどのように変化するか調査する。複素および倍次元の実数ニューラルネットワークとしてフィードフォワード型層状ニューラルネットワークを取り上げる。簡潔な例として、それらが時間信号に対して関数近似あるいはフィルタ的な動作をするときの汎化特性を調べる。計算機実験の結果、複素ニューラルネットワークは倍次元実数ニューラルネットワークに比べて、その汎化特性が良好であり、特に信号の波動性が高い場合にその差が顕著であることが確認される。

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