Updated on 2024/09/20

写真a

 
KATO-MINOURA Takako
 
Organization
Faculty of Science and Engineering Professor
Other responsible organization
Biological Sciences Course of Graduate School of Science and Engineering, Master's Program
Biological Sciences Course of Graduate School of Science and Engineering, Doctoral Program
External link

Degree

  • Doctor of Science ( Nagoya University )

  • Master of Science ( Nagoya University )

Education

  • 1997.3
     

    Nagoya University   Graduate School of Science   Division of Molecular Biology   doctor course   finished without a degree after completion of required course credits

  • 1994.3
     

    Nagoya University   Graduate School of Science   Division of Molecular Biology   master course   completed

  • 1991.3
     

    Nagoya University   School of Science   Department of Molecular Biology   graduated

Research History

  • 2022.4 - 　

    Chuo University   Faculty of Science and Engineering   Professor

  • 2008.4 - 2022.3

    Chuo University   Faculty of Science and Engineering   Associate Professor

  • 2007.4 - 2008.3

    Gakushuin University   Faculty of Science   Research Associate

  • 2003.12 - 2007.3

    The University of Tokyo   "Department of Life Sciences, Graduate School of Arts and Sciences"   Research Associate

  • 2002.4 - 2003.12

    University of Tsukuba   Institute of Biological Sciences   Technical Official

  • 1997.4 - 2002.3

    National Institute for Basic Biology   Research Associate

  • 1996.4 - 1997.3

    Nagoya University   Faculty of Science   JSPS Research Fellowship for Young Scientists (DC2)

  • 1994.11 - 1994.12

    Jichi Medical University   植物性機能生理学実習 講師

  • 1994.5 　 　

    Jichi Medical University   植物性機能生理学実習 講師

  • 1992.4 - 1993.3

    University of Minnesota   Department of Cell Biology and Neuroanatomy   Pre-doctoral Assistant

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Professional Memberships

  • The Biophysical Society of Japan

  • The Zoological Society of Japan

  • Japan Society for Cell Biology

  • American Society for Cell Biology

Research Interests

  • Cell motility

  • Cytoskeleton

  • actin

  • Cilia/Flagella

Research Areas

  • Life Science / Biophysics  / Biophysics

  • Life Science / Cell biology  / Cell biology

  • Life Science / Functional biochemistry  / Functional biochemistry

  • Life Science / Evolutionary biology  / Evolutionary biology

Papers

  • IC2 participates in the cooperative activation of outer arm dynein densely attached to microtubules Reviewed

    Yusuke Kondo, Tomoka Ogawa, Emiri Kanno, Masafumi Hirono, Takako Kato-Minoura, Ritsu Kamiya, Toshiki Yagi

    Cell Structure and Function   48   175 - 185   2023

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Cell Biology  

    DOI： 10.1247/csf.23044

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  • Chlamydomonas reinhardtii tubulin-gene disruptants for efficient isolation of strains bearing tubulin mutations. Reviewed

    Kato-Minoura, T, Ogiwara, Y, Yamano, T, Fukuzawa, H, Kamiya, R

    PLOS ONE   15 ( 11 )   e0242694 - e0242694   2020.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Public Library of Science  

    The single-cell green alga <italic>Chlamydomonas reinhardtii</italic> possesses two α-tubulin genes (<italic>tua1</italic> and <italic>tua2</italic>) and two β-tubulin genes (<italic>tub1</italic> and <italic>tub2</italic>), with the two genes in each pair encoding identical amino acid sequences. Here, we screened an insertional library to establish eight disruptants with defective <italic>tua2</italic>, <italic>tub1</italic>, or <italic>tub2</italic> expression. Most of the disruptants did not exhibit major defects in cell growth, flagellar length, or flagellar regeneration after amputation. Because few tubulin mutants of <italic>C</italic>. <italic>reinhardtii</italic> have been reported to date, we then used our disruptants, together with a <italic>tua1</italic> disruptant obtained from the <italic>Chlamydomonas</italic> Library Project (CLiP), to isolate tubulin-mutants resistant to the anti-tubulin agents propyzamide (pronamide) or oryzalin. As a result of several trials, we obtained 8 strains bearing 7 different α-tubulin mutations and 12 strains bearing 7 different β-tubulin mutations. One of the mutations is at a residue similar to that of a mutation site known to confer drug resistance in human cancer cells. Some strains had the same amino acid substitutions as those reported previously in <italic>C</italic>. <italic>reinhardtii</italic>; however, the mutants with single tubulin genes showed slightly stronger drug-resistance than the previous mutants that express the mutated tubulin in addition to the wild-type tubulin. Such increased drug-resistance may have facilitated sensitive detection of tubulin mutation. Single-tubulin-gene disruptants are thus an efficient background of generating tubulin mutants for the study of the structure–function relationship of tubulin.

    DOI： 10.1371/journal.pone.0242694

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  • SAXS signals from double-stranded DNA under shear-flow conditions. Reviewed

    Fujita, Y, Kato-Minoura, T, Alessandro, L, Wada, Y, Yagi, T, Iwamoto, H, Kamiya, R, Kamimura, S

    SPring-8/SACLA Research Report   8 ( 3 )   469 - 472   2020.10

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  • Changes in the cell shape and actin organization accompanying primary ciliogenesis in Xenopus epithelial cells. Reviewed

    Ishigami, R, Kato-Minoura, T

    Journal of the Institute of Science and Engineering. Chuo University   24   1 - 11   2019.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Institute of Science and Engineering. Chuo University  

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  • Release of sticky glycoproteins from Chlamydomonas flagella during microsphere translocation on the surface membrane. Reviewed

    Kamiya, R, Shiba, K, Inaba, K, Kato-Minoura, T

    Zoological Science   35   299 - 305   2018.8

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Zoological Society of Japan  

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  • Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. Reviewed

    Kato-Minoura, T, Karino, K, Akimoto, N, Yoshiga, N, Ehara, M. Aoki

    Plant Systematics and Evolution   301   1725 - 1733   2015.1

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer International Publishing AG  

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  • Extremely low polymerizability of a highly-divergent Chlamydomonas actin (NAP) Reviewed

    Takako Kato-Minoura

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   412 ( 4 )   723 - 727   2011.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Novel actin-like protein (NAP) is a highly divergent actin expressed in Chlamydomonas. With its low sequence similarity, it is uncertain whether NAP can polymerize into filaments. Here I assessed it by ectopically expressing enhanced green fluorescent protein-tagged NAP (EGFP-NAP) in cultured cells. EGFP-NAP was excluded from stress fibres but partially co-localized with endogenous actin in the cell periphery. In fluorescence recovery after photobleaching experiment, turnover rate of EGFP-NAP was similar to the estimated diffusion rate of monomeric actin. Therefore, EGFP-NAP likely accumulates by diffusion. These findings suggest that NAP has extremely poor ability to polymerize. (C) 2011 Elsevier Inc. All rights reserved.

    DOI： 10.1016/j.bbrc.2011.08.040

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  • 真核生物の鞭毛・繊毛形成の制御機構の解明（遺伝子発現と鞭毛内輸送IFTの連関について）

    箕浦高子

    平成20年度研究助成金受給者研究報告集   27   111 - 115   2010.3

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    Language：Japanese   Publisher：財団法人日揮・実吉奨学会  

    CiNii Books

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  • Identification of a novel actin-related protein in Tetrahymena cilia Reviewed

    S Kuribara, M Kato, T Kato-Minoura, O Numata

    CELL MOTILITY AND THE CYTOSKELETON   63 ( 7 )   437 - 446   2006.7

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-LISS  

    Actin is an ancient cytoskeletal protein that plays many essential roles in cell motility. In eukaryotes, its gene belongs to a highly conserved gene family, while the protein is also a member of an actin superfamily comprising various kinds of actin-related proteins (Arps). A ciliate, Tetrahymena, has a unique conventional actin. Data from the TIGR Tetrahymena genome project and our own research suggest the existence of 12 actin-like sequences: four conventional actins, two of Arp4, one each of Arp1, Arp2, Arp3, Arp5, and Arp6, and a novel actin-related protein, tArp. We cloned the entire cDNA sequences of Tetrahymena Arp2 (tArp2), Tetrahymena Arp3 (tArp3), and tArp for the work described herein. In phylogenetic analyses, tArp was not included in any Arp, subfamily. Unlike other known Arps, tArp localizes in cilia, and its expression was upregulated after deciliation. To see the precise localization of tArp, cilia were fractionated and analyzed using specific antibodies. tArp was observed preferentially in the "outer-doublet" fraction, while actin was found in the "crude-dynein" fraction. In immunoelectron microscopy, most of the gold particles were found either on the outer-doublet or central-pair microtubules. These results suggest that tArp is a ciliary component and that it has a unique function in the formation and maintenance of cilia.

    DOI： 10.1002/cm.20136

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  • 2P223 Identification of a novel actin-related protein in Tetrahymena cilia

    Kato-Minoura,T, Kuribara,S, Kato,M, Numata,O

    Biophysics   45 ( 1 )   0   2005.10

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：The Biophysical Society of Japan  

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  • Impaired flagellar regeneration due to uncoordinated expression of two divergent actin genes in Chlamydomonas Reviewed

    T Kato-Minoura

    ZOOLOGICAL SCIENCE   22 ( 5 )   571 - 577   2005.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ZOOLOGICAL SOC JAPAN  

    Chlamydomonas has two actin genes: one encoding a conventional actin (90% amino acid identity with mammalian actin) and the other a highly divergent actin (NAP; 64% identity). The expression of the two genes is regulated in a mutually exclusive manner. Thus, ida5, a mutant that lacks the conventional actin (CrA) gene, expresses NAP abundantly, whereas wild-type cells express NAP only negligibly under normal conditions. To explore the physiological significance of the two actins, chimeric genes with the 5' upstream region of one gene replaced by that of the other were constructed and used to transform ida5. The transformant (TF5) with a chimeric clone comprising the 5'-untranslated region from the NAP gene and the CrA-encoding sequence recovered the dyneins missing in ida5 and showed almost normal motility. After deflagellation of this transformant, however, only about 30% of cells grew flagella, unlike wildtype cells, &gt; 80% of which displayed reflagellation. Transformant TF10, which contains the CrA upstream region and NAP coding region, underwent reflagellation normally, as did the parent strain, ida5. In TF5, the mRNA level of both CrA and NAP was increased greatly during reflagellation. In light of the recent finding that NAP mRNA is expressed transiently upon reflagellation in wild-type cells, the described results suggest that 1) the expression of NAP mRNA is indispensable for flagellation and 2) robust expression of CrA may inhibit proper flagellation by interfering with the function of NAP in the early stages of reflagellation.

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  • A novel family of unconventional actins in volvocalean algae Reviewed

    T Kato-Minoura, M Okumura, M Hirono, R Kamiya

    JOURNAL OF MOLECULAR EVOLUTION   57 ( 5 )   555 - 561   2003.11

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER-VERLAG  

    The unicellular green alga Chlamydomonas reinhardtii has two actin genes, one encoding a conventional actin (90% amino acid identity with mammalian actin), the other a highly divergent actin (64% identity) named novel actin-like protein (NAP). To see whether the presence of conventional and unconventional actins in a single organism is unique to C. reinhardtii, we searched for genomic sequences related to the NAP sequence in several other species of volvocalean algae. Here we show that Chlamydomonas moewusii and Volvox carteri also have, in addition to a conventional actin, an unconventional actin similar to the C. reinhardtii NAP. Analyses of the deduced protein sequences indicated that the NAP homologues form a distinct group derived from conventional actin.

    DOI： 10.1007/s00239-003-2509-3

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  • Expression of conventional and unconventional actins in Chlamydomonas reinhardtii upon deflagellation and sexual adhesion Reviewed

    M Hirono, S Uryu, A Ohara, T Kato-Minoura, R Kamiya

    EUKARYOTIC CELL   2 ( 3 )   486 - 493   2003.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER SOC MICROBIOLOGY  

    Chlamydomonas has two actin genes, one coding for a conventional actin and the other coding for a highly divergent actin. The divergent actin NAP (for "novel actin-like protein") is expressed only negligibly in wild-type cells but abundantly in a null mutant of conventional actin, the ida5 mutant. The presence of the dormant NAP gene suggests that NAP may also have its own function in wild-type cells under some conditions. However, no specific functions have been suggested. In this study, we examined the expression of actin and NAP in wild-type and ida5 cells under conditions where actin function has been shown to be important. We found that deflagellation induces the expression of NAP as well as that of actin in wild-type cells. The expressed NAP becomes localized to the regrown flagella, apparently without being associated with dynein. Mating of gametes also increased the expression of actin in wild-type cells and that of NAP in ida5 cells, resulting in accumulation of these proteins in flagella (in both wild-type and ida5 cells) and the fertilization tubule (only in wild-type cells). However, it did not induce significant NAP expression in wild-type cells. These and other observations suggest that the expression of actin and NAP mRNAs is controlled by two discrete mechanisms and that NAP plays a role in flagellar formation in wild-type cells.

    DOI： 10.1128/EC.2.3.486-4912003

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  • Recovery of flagellar inner-arm dynein and the fertilization tubule in Chlamydomonas ida5 mutant by transformation with actin genes Reviewed

    A Ohara, T Kato-Minoura, R Kamiya, M Hirono

    CELL STRUCTURE AND FUNCTION   23 ( 5 )   273 - 281   1998.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPAN SOC CELL BIOLOGY  

    The ida5 mutant of Chlamydomonas, first isolated as a mutant lacking a subset of axonemal inner-arm dyneins, has recently been shown to lack conventional actin owing to a serious mutation in its gene. It lacks inner-arm dyneins probably because actin is an essential subunit for their assembly. In addition, male gametes of ida5 are unable to produce the fertilization tubule, a structure that contains a core of actin filament bundles. To establish that those observed deficiencies are solely attributable to the loss of actin, and to provide a basis for future studies on the actin function in this organism, we examined in this study whether transformation of this mutant with cloned actin genes can rescue the mutant phenotypes. Cotransformation of the double mutant ida5arg2 with the wild-type actin gene and arginino-succinate lyase gene that suppresses the arg2 mutation yielded several transformants that displayed increased motility. All of them were found to have acquired the introduced actin gene In the genome and the product actin in the flagella, and regained the missing inner-arm dyneins and wild-type motility. In addition, most transformants also became able to grow the fertilization tubule when mating reaction was induced. In addition to the wild-type actin gene, we also used a chimeric actin gene in which the N-terminal Y2 amino-acid sequence of Chlamydomonas actin was replaced by that of the greatly divergent Tetrahymena actin. Transformants with this gene also resulted in recovery of inner-arm dynein and 70-80% of the wild-type level of motility. These results established that the lack of inner-arm dynein and the fertilization tubule in ida5 are consequences of its loss of conventional actin. Furthermore, they demonstrate that Chlamydomonas offers an excellent experimental system with which to study the structure-function relationship of actin by means of mutant analysis.

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  • Highly divergent actin expressed in a Chlamydomonas mutant lacking the conventional actin gene Reviewed

    T Kato-Minoura, S Uryu, M Hirono, R Kamiya

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   251 ( 1 )   71 - 76   1998.10

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACADEMIC PRESS INC  

    The Chlamydomonas mutant ida5 is deficient in the conventional actin gene and its axoneme lacks a subset of inner dynein arms that contain actin as a subunit. However, this mutant retains some other inner dynein arms because a novel protein (NAP) is expressed as a substitute for actin. In this study, we show by sequence analysis that NAP is identical to a putative actin-related protein, the cDNA sequence of which has recently been reported and shown to have 64% amino acid identity with conventional actin. A polyclonal antibody raised against a synthetic polypeptide corresponding to the NH2-terminal sequence of this protein specifically reacted with the spot corresponding to NAP in two-dimensional electrophoresis patterns. NAP apparently can substitute for conventional actin in some, but not all, cellular functions, and therefore can be regarded as a highly divergent actin. This unconventional actin appears to be expressed only when conventional actin is absent. (C) 1998 Academic Press.

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  • Chlamydomonas inner-arm dynein mutant, ida5, has a mutation in an actin-encoding gene Reviewed

    T KatoMinoura, M Hirono, R Kamiya

    JOURNAL OF CELL BIOLOGY   137 ( 3 )   649 - 656   1997.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ROCKEFELLER UNIV PRESS  

    Chlamydomonas flagellar inner-arm dynein consists of seven subspecies (a-g), of which all but f contain actin as subunits. The mutant ida5 and a new strain, ida5-t, lack four subspecies (a, c, d, and e). These mutants were found to have mutations in the conventional actin gene, such that its product is totally lost; ida5 has a single-base deletion that results in a stop codon at a position about two-thirds from the 5' end of the coding region, and idn5-t lacks a large portion of the entire actin gene. Two-dimensional gel electrophoresis patterns of the axonemes and inner-arm subspecies b and g of ida5 lacked the spot of actin (isoelectric point [pI] = similar to 5.3) but had two novel spots with pIs of similar to 5.6 and similar to 5.7 instead. Western blot with different kinds of anti-actin antibodies suggested that the proteins responsible for the two novel spots and conventional actin are different but share some antigenicity. Since Chlamydomonas has been shown to have only a single copy of the conventional actin gene, it is likely that the novel spots in ida5 and ida5-t originated from another gene(s) that codes for a novel actin-like protein(s) (NAP), which has hitherto been undetected in wildtype cells. These mutants retain the two inner-arm subspecies b and g, in addition to f, possibly because NAP can functionally substitute for the actin in these subspecies while they cannot in other subspecies. The net growth rate of ida5 and ida5-t cells did not differ from that of wild type, but the mating efficiency was greatly reduced. This defect was apparently caused by deficient growth of the fertilization tubule. These results suggest that NAP can carry out some, but not all, functions performed by conventional actin in the cytoplasm and raise the possibility that Chlamydomonas can live without ordinary actin.

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  • CHARACTERIZATION OF A MINUS END-DIRECTED KINESIN-LIKE MOTOR PROTEIN FROM CULTURED-MAMMALIAN-CELLS Reviewed

    R KURIYAMA, M KOFRON, R ESSNER, T KATO, S DRAGASGRANOIC, CK OMOTO, A KHODJAKOV

    JOURNAL OF CELL BIOLOGY   129 ( 4 )   1049 - 1059   1995.5

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：ROCKEFELLER UNIV PRESS  

    Using the CHO2 monoclonal antibody raised against CHO spindles (Sellitto, C., M. Kimble, and R. Kuriyama. 1992. Cell Motil. Cytoskeleton. 22:7-24) we identified a 66-kD protein located at the interphase centrosome and mitotic spindle. Isolated cDNAs for the antigen encode a 622-amino acid polypeptide. Sequence analysis revealed the presence of 340-amino acid residues in the COOH terminus, which is homologous to the motor domain conserved among other members of the kinesin superfamily. The protein is composed of a central alpha-helical portion with globular domains at both NH2 and COOH termini, and the epitope to the monoclonal antibody resides in the central alpha-helical stalk. A series of deletion constructs were created for in vitro analysis of microtubule interactions. While the microtubule binding and bundling activities require both the presence of the COOH terminus and the alpha-helical domain, the NH2-terminal half of the antigen lacked the ability to interact with microtubules. The full-length as well as deleted proteins consisting of the COOH-terminal motor and the central alpha-helical stalk supported microtubule gliding, with velocity ranging from 1.0 to 8.4 mu m/minute. The speed of microtubule movement decreased with decreasing lengths of the central stalk attached to the COOH-terminal motor. The microtubules moved with their plus end leading, indicating that the antigen is a minus end-directed motor. The CHO2 sequence shows 86% identify to HSET, a gene located at the centromeric end of the human MHC region in chromosome 6 (Ando, A., Y. Y. Kikuti, H. Kawata, N. Okamoto, T. Imai, T. Eki, K. Yokoyama, E. Soeda, T. Ikemura, K. Abe, and H. Inoko. 1994. Immunogenetics. 39:194-200), indicating that HSET might represent a human homologue of the CHO2 antigen.

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  • ISOLATION OF 2 SPECIES OF CHLAMYDOMONAS-REINHARDTII FLAGELLAR MUTANTS, IDA5 AND IDA6, THAT LACK A NEWLY IDENTIFIED HEAVY-CHAIN OF THE INNER DYNEIN ARM Reviewed

    T KATO, O KAGAMI, T YAGI, R KAMIYA

    CELL STRUCTURE AND FUNCTION   18 ( 6 )   371 - 377   1993.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPAN SOC CELL BIOLOGY  

    Two novel Chlamydomonas mutants, ida5 and ida6, that lack subsets of inner-arm dynein have been isolated and mapped to discrete loci on the right arm of linkage group XIV. Of the seven different inner-arm dynein subspecies (a, b, c, d, e, f and g) identified by ion-exchange chromatography, ida5 lacks a, c, d and e, while ida6 lacks e alone; these are the only mutants that have been shown to lack subspecies e. Both strains can swim, albeit more slowly than the wild type. Hence, subspecies e must contribute-to flagellar movement although it is unnecessary for the generation of undulating movement.

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Books

  • 分子細胞生物学

    箕浦 高子（ Role： Sole author）

    デザインエッグ社  2023.5  （ ISBN:4815003823 ）

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    Total pages：194   Language：Japanese  

    ASIN

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  • 分子遺伝学: Molecular Genetics

    箕浦 高子（ Role： Sole author）

    デザインエッグ社  2023.2  （ ISBN:4815003203 ）

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    Total pages：170   Language：Japanese  

    ASIN

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  • ベーシックマスター細胞生物学

    尾張部克志, 神谷律, 共編, 植松淳, 奥野誠, 小保方潤一, 加藤幹男, 上村慎治, 小林剛, 塩尻信義, 鈴木信太郎, 坪井貴司, 久本直毅, 廣野雅文, 古橋潔, 升田裕久, 箕浦高子（ Role： Edit第8章 細胞骨格と細胞運動）

    オーム社  2009.8 

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    Total pages：327   Responsible for pages：164-192   Language：Japanese   Book type：Scholarly book

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  • Introductory Course in Life Science Experiments 2nd edition

    東京大学教養学部基礎生命科学実験編集委員会（ Role： Edit）

    東京大学出版会  2009.2 

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    Total pages：224   Language：Japanese   Book type：Scholarly book

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  • Introductory Course in Life Science Experiments

    東京大学教養学部基礎生命科学実験編集委員会（ Role： Edit主要分担執筆およびDVD編集：第Ⅱ編 細胞の動的構造と機能、実験5 単細胞生物の構造と細胞小器官の機能-ゾウリムシの観察、実験6 繊毛運動と生体エネルギー-ゾウリムシの細胞モデル）

    東京大学出版会  2007.1 

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    Total pages：205   Language：Japanese   Book type：Scholarly book

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MISC

  • アカデミックハラスメントの現状と、今私たちにできること

    箕浦高子

    つくば生物ジャーナル   2 ( 9 )   316 - 317   2003.9

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (other)   Publisher：筑波大学生物学類  

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  • A novel actin family in volvocalean

    T Kato-Minoura, M Okumura, M Hirono, R Kamiya

    MOLECULAR BIOLOGY OF THE CELL   12   32A - 32A   2001.11

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    Language：English   Publishing type：Research paper, summary (international conference)   Publisher：AMER SOC CELL BIOLOGY  

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  • 年会参加のハードルを下げる:保育室設置の試み

    箕浦高子

    生物物理   41 ( 2 )   117 - 119   2001.4

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (other)   Publisher：日本生物物理学会  

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  • Chlamydomonas flagellar inner-arm dynein mutant, ida5, expresses a highly diverged actin

    T KatoMinoura, S Uryu, M Hirono, R Kamiya

    MOLECULAR BIOLOGY OF THE CELL   8   212 - 212   1997.11

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    Language：English   Publishing type：Research paper, summary (international conference)   Publisher：AMER SOC CELL BIOLOGY  

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Presentations

  • Dosage compensation of the Chlamydomonas tubulin genes

    Shiori Uchiyama, Kaho Higuchi, Takako Kato-Minoura

    2024.1 

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    Event date： 2024.1 　 　

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Dosage compensation of the Chlamydomonas tubulin genes

    Shiori Uchiyama, Kaho Higuchi, Ritsu Kamiya, Takako Kato-MInoura

    2023.9 

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    Event date： 2023.9 　 　

    Language：Japanese   Presentation type：Oral presentation (general)  

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  • Disturbance of motor activity of outer-arm dynein by a point mutation in intermediate chain 2

    Yusuke Kondo, Tomoka Ogawa, Emiri Kanno, Masafumi Hirono, Takako Kato-Minoura, Stephan M. King, Ritsu Kamiya, Toshiki Yagi

    The 20th International Conference on the Cell and Molecular Biology of Chlamydomonas  2023.6 

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    Event date： 2023.6 　 　

    Language：English   Presentation type：Poster presentation  

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  • The effect of the HSP40 chaperone Apm1 on Chlamydomonas flagellar assembly

    Momoka Yamaguchi, Takako Kato-Minoura

    2023.3 

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    Event date： 2023.3 　 　

    Language：Japanese   Presentation type：Poster presentation  

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  • Relative abundance of Chlamydomonas tubulin and Apm2/Apm1 (an Hsp70/40 chaperone) in different subcellular compartments under the influence of anti-tubulin drugs

    Karen Furukawa, Takako Kato-Minoura

    2023.3 

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  • Inhibition of deflagellation by KCA1, a calcium-activated potassium channel

    Rina Takahashi, Yuma Asahina, Akitoshi Usami, Shiori Uchiyama, Takako Kato-Minoura, Kenjiro Yoshimura, Toru Hisabori, Ken-ichi Wakabayashi

    2023.3 

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  • Interaction of microtubules and Apm2/Apm1, an Hsp70/40 chaperone in Chlamydomonas

    Miyu Kawase, Karen Furukawa, Momoka Yamaguchi, Takako Kato-Minoura

    2023.3 

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  • Interaction of microtubules and Apm2/Apm1, an Hsp70/40 chaperone in Chlamydomonas

    Miyu Kawase, Karen Furukawa, Momoka Yamaguchi, Takako Kato-Minoura

    2023.1 

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  • Cooperative interactions between axonemal dyneins are regulated by the intermediate chain 2 of outer-arm dynein

    Yusuke Kondo, Tomoka Ogawa, Emiri Kanno, Masafumi Hirono, Takako Kato-Minoura, Ritsu Kamiya, Toshiki Yagi

    The 60th Annual Meeting of the Biophysical Society of Japan  2022.9 

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  • Cooperative interactions between outer-arm dyneins are regulated by the dynein intermediate chain 2

    Yusuke Kondo, Tomoka Ogawa, Emiri Kanno, Masafumi Hirono, Takako Kato-Minoura, Ritsu Kamiya, Toshiki Yagi

    The 93rd Annual Meeting of the Zoological Society of Japan  2022.9 

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  • Isolation of Chlamydomonas tubulin mutants resistant to a tubulin depolymerization inhibitor

    Kaoru Haneda, Ritsu Kamiya, Takako Kato-Minoura

    第15回クラミドモナス研究会  2022.3 

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  • A novel Chlamydomonas mutant harboring a point mutation in Intermediate Chain 2 of outer-arm dynein displays lowered motility International conference

    Yusuke Kondo, Tomoka Ogawa, Emiri Kanno, Masafumi Hirono, Takako Kato-Minoura, Ritsu Kamiya, Toshiki Yagi

    Dynein 2021 International Workshop  2021.9 

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  • Efficient isolation of Chlamydomonas tubulin mutants using strains that have single alpha- and beta-tubulin genes International conference

    Takako Kato-Minoura, Yutaro Ogiwara, Hideya Fukuzawa, Takashi Yamano, Ritsu Kamiya

    19th International Conference on the Cell & Molecular Biology of Chlamydomonas  2021.8 

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  • Efficient isolation of Chlamydomonas tubulin mutants using strains that have single alpha- and beta-tubulin genes

    Takako Kato-Minoura, Yutaro Ogiwara, Takashi Yamano, Hideya Fukuzawa, Ritsu Kamiya

    第14回クラミドモナス研究会  2021.3 

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  • Efficient isolation of Chlamydomonas tubulin mutants using strains that have single alpha- and beta-tubulin genes

    Takako Kato-Minoura, Yutaro Ogiwara, Takashi Yamano, Hideya Fukuzawa, Ritsu Kamiya

    第14回クラミドモナス研究会  2021.3 

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  • Possible regulation of tubulin/microtubules by an HSP70/40 molecular chaperone Apm1/Apm2

    Karen Furukawa, Momoka Yamaguchi, Miyu Kawase, Kaho Kiuchi, Minami Horie, Takako Kato-Minoura

    第14回クラミドモナス研究会  2021.3 

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  • Mutations in Chlamydomonas Apm1 (a member of HSP40 family) confer resistance to anti-tubulin agents.

    Takako Kato-Minoura, Ritsu Kamiya

    2020年生体運動研究合同班会議  2020.1 

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  • ボルボックス系列異型配偶ユードリナの性フェロモンによる配偶子誘導

    豊岡 博子, 浜地 貴志, 西村 芳樹, 宮城島 進也, 箕浦 高子, 野崎 久義

    The 83rd Annual Meeting of the Botanical Society of Japan  2019.9 

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  • Sex pheromone-induced gametogenesis in anisogamous Eudorina International conference

    Hiroko Kawai-Toyooka, Takashi Hamaji, Yoshiki Nishimura, Shin-ya Miyagishima, Takako Kato-Minoura, Hisayoshi Nozaki

    5th International Volvox Conference  2019.7 

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  • Efficient isolation of Chlamydomonas tubulin mutants from strains lacking one each of its two alpha-tubulin and two beta-tubulin genes International conference

    Takako Kato-Minoura, Yutaro Ogiwara, Ryosuke Suzuki, Mami Ishigaki, Yidong Tang, Ritsu Kamiya

    5th International Volvox Conference  2019.7 

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  • Roles of a molecular chaperone, Apm1 that associates with resistance to tubulin polymerization inhibitors in Chlamydomonas.

    Fumika Tanaka, Miki Takenaka, Ritsu Kamiya, Takako Kato-Minoura

    The 71st Annual Meeting of the Kanto Branch of Zoological Society of Japan  2019.3 

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  • Roles of high-molecular-weight glycoproteins, FAP113 and FMG-1B that contributes to gliding motiliy in Chlamydomonas flagella.

    Ken'ya, Miyazaki, Misaki Ikeda, Ritsu Kamiya, Takako Kato-Minoura

    The 71st Annual Meeting of the Kanto Branch of Zoological Society of Japan  2019.3 

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  • Isolation of Chlamydomonas alpha- or beta-tubulin mutants from a strain that lacks one each of the two alpha- or two beta-tubulin genes.

    Takako Kato-Minoura, Ritsu Kamiya

    2019年生体運動研究合同班会議  2019.1 

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  • A point mutation in intermediate chain gene reduces motor activity of outer-arm dynein.

    Yusuke Kondo, Tomoka Ogawa, Emiri Kanno, Masafumi Hirono, Takako Kato-Minoura, Ritsu Kamiya, Toshiki Yagi

    The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

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  • ボルボックス系列異型配偶ユードリナにおける配偶子誘導要因の解析

    豊岡 博子, 浜地 貴志, 西村 芳樹, 宮城島 進也, 箕浦 高子, 野崎 久義

    The 82nd Annual Meeting of the Botanical Society of Japan  2018.9 

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  • Properties of Chlamydomonas mutants that lack one of the two alpha- or two beta-tubulin genes International conference

    Takako Kato-Minoura, Yutaro Ogiwara, Ryosuke Suzuki, Hideya Fukuzawa, Takashi Yamano, Ritsu Kamiya

    18th International Conference on the Cell & Molecular Biology of Chlamydomonas  2018.7 

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  • Properties of Chlamydomonas mutants that lack one each of the two alpha- or two beta-tubulin genes.

    Kato-Minoura, Takako

    2018年生体運動研究合同班会議  2018.1 

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  • Rearrangement of actin cytoskeleton during primary ciliogenesis in Xenopus epithelial cells.

    Riho Ishigami, Takako Kato-Minoura

    The 88th Annual Meeting of the Zoological Society of Japan  2017.9 

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  • A novel Chlamydomonas mutant harboring a point mutation in an intermediate chain gene of outer-arm dynein displays lowered motility.

    Tomoka Ogawa, Emiri Kanno, Yusuke Kondo, Masafumi Hirono, Takako Kato-Minoura, Ritsu Kamiya, Toshiki Yagi

    The 88th Annual Meeting of the Zoological Society of Japan  2017.9 

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  • A novel Chlamydomonas mutant harboring a point mutation in an intermediate chain gene of outer-arm dynein displays lowered motility.

    Tomoka Ogawa, Emiri Kanno, Yusuke Kondo, Masafumi Hirono, Takako Kato-Minoura, Ritsu Kamiya, Toshiki Yagi

    The 55th Annual Meeting of the Biophysical Society of Japan  2017.9 

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  • Characteristic actin structures found in Xenopus epithelial cells.

    Ishigami, Riho, Kato-Minoura, Takako

    第12回細胞運動研究会  2017.9 

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  • Surface motility in eukaryote cilia/flagella: Generality and membrane protein dynamics

    Ritsu Kamiya, Kogiku Shiba, Kazuo Inaba, Takako Kato-Minoura

    The 54th Annual Meeting of the Biophysical Society of Japan  2016.11 

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  • Isolation of Chlamydomonas mutants that lack one of the two alpha-tubulin or two beta-tubulin genes

    Takako Kato-Minoura, Hideya Fukuzawa, Takashi Yamano

    The 22nd International Congress of Zoology  2016.11 

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  • Ciliogenesis in Xenopus epithelial cells may depend on novel actin- containing structures formed at the basal region

    Riho Ishigami, Takako Kato-Minoura

    The 22nd International Congress of Zoology  2016.11 

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  • Isolation of Chlamydomonas mutants that lack one of the two alpha-tubulin or two beta-tubulin genes

    Takako Kato-Minoura, Hideya Fukuzawa, Takashi Yamano

    EMBO conference on Cilia 2016  2016.10 

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  • Phylogenetic analysis of NAP, an unconventional actin of the Volvocales

    Takako Kato-Minoura, Seishiro Aoki

    17th International Conference on the Cell & Molecular Biology of Chlamydomonas  2016.6 

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  • N-terminal sequence of actin is critical for the assembly of flagellar inner-arm dyneins

    Takako Kato-Minoura, Yuko Horikoshi, Kaori Imaeda

    The 86th Annual Meeting of the Zoological Society of Japan  2015.9 

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  • N-terminal sequence of actin is critical for the assembly of flagellar inner-arm dyneins

    Takako Kato-Minoura, Yuko Horikoshi, Kaori Imaeda

    第12回クラミドモナス研究会  2015.9 

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  • Phylogenetic analysis of NAP, an unconventional actin of the Volvocales

    Takako Kato-Minoura, Kumiko Karino, Nobuyuki Akimoto, Norito Yoshiga, Mika Ehara, Seishiro Aoki

    第11回クラミドモナス研究会  2014.10 

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  • 緑藻ボルボックス目における光反応行動調節機構

    植木紀子, 箕浦高子, 若林憲一

    第11回クラミドモナス研究会  2014.10 

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  • Evaluation of actin subdomains responsible for formation of the inner-arm dyneins

    Kaori Imaeda, Yuko Horikoshi, Takuya Satomura, Takako Kato-Minoura

    第11回クラミドモナス研究会  2014.10 

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  • Evaluation of actin subdomains responsible for formation of the inner-arm dyneins

    Kaori Imaeda, Yuko Horikoshi, Takuya Satomura, Takako Kato-Minoura

    第９回細胞運動研究会  2014.9 

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  • Evaluation of actin subdomains responsible for formation of the inner-arm dyneins

    Kaori Imaeda, Yuko Horikoshi, Takako Kato-Minoura

    The 85th Annual Meeting of the Zoological Society of Japan  2014.9 

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  • Dynamics of double helix DNA under shear flow

    Yosuke Fujita, Nobumasa Nakazawa, Takako Kato-Minoura, Hiroyuki Iwamoto, Shinji Kamimura

    The 52nd Annual Meeting of the Biophysical Society of Japan  2014.9 

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  • Effects of an actin gene with an altered regulatory region on the flagellar formation and motility in Chlamydomonas

    Yoshito Masuda, Takako Kato-Minoura

    The 84th Annual Meeting of the Zoological Society of Japan  2013.9 

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  • Molecular Evolution of Volvocalean Actin Genes

    Takako Kato-Minoura, Mika Ehara, Kumiko Karino, Nobuyuki Akimoto, Norito Yoshiga, Seishiro Aoki

    The 84th Annual Meeting of the Zoological Society of Japan  2013.9 

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  • Molecular evolution of volvocalean actin genes

    Takako Kato-Minoura, Mika Ehara, Kumiko Karino, Nobuyuki Akimoto, Norito Yoshiga, Seishiro Aoki

    The 2nd International Volvox Conference  2013.8 

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  • The effect of Nano Bubbles on the growth of amphibian cutured cells

    Haruka Makino, Takako Kato-Minoura, Shinji Kamimura, Masayuki Ohmori

    The 83th Annual Meeting of the Zoological Society of Japan  2012.9 

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  • Phylogenic analyses have revealed a unique origin of NAP, a Volvocales unconventional actin, in Chlolophyta

    Takako Kato-Minoura, Kumiko Karino, Nobuyuki Akimoto, Norito Yoshiga, Seishiro Aoki

    Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology  2012.5 

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  • Extremely low polymerizability of a highly-divergent Chlamydomonas actin (NAP) as examined by ectopic expression in amphibian cultured cells

    Kato-Minoura, Takako

    The American Society for Cell Biology 2011 Annual Meeting  2011.12 

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  • Molecular evolution of volvocalean-specific actin homologs (NAP).

    Takako Kato-Minoura, Seishiro Aoki

    The 82nd Annual Meeting of the Zoological Society of Japan  2011.9 

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  • A FRAP analysis of ectopically expressed Chlamydomonas unconventional actin, NAP.

    Kato-Minoura, Takako

    The 63rd Annual Meeting of the Japan Society for Cell Biology  2011.6 

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  • A FRAP analysis of ectopically expressed Chlamydomonas unconventional actin, NAP.

    Kato-Minoura, Takako

    2011年生体運動研究合同班会議  2011.1 

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  • NAP, an unconventional actin in Volvocales.

    Takako Kato-Minoura, Seishiro Aoki

    第8回クラミドモナスワークショップ  2010.12 

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  • FRAP analysis of ectopically expressed NAP, a Chlamydomonas unconventional actin

    Kato-Minoura, Takako

    The 81st Annual Meeting of the Zoological Society of Japan  2010.9 

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  • Polymerization activity of ectopically expressed NAP, a Chlamydomonas unconventional actin

    Takako Kato-Minoura

    The 80th Annual Meeting of the Zoological Society of Japan  2009.9 

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  • Chlamydomnas unconventional actin, NAP, can polymerize in vivo.

    Kato-Minoura, Takako

    The 61st Annual Meeting of the Japan Society for Cell Biology  2009.6 

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  • NAP, an unconventional actin in green algae.

    Kato-Minoura, Takako

    2009年生体運動研究合同班会議  2009.1 

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  • Observation of the Contractile Ring using Photoconvertible GFP Variants

    Takako Kato-Minoura, Issei Mabuchi

    The 78th Annual Meeting of the Zoological Society of Japan  2007.9 

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  • Introductory course in life science experiments at the University of Tokyo: Development of a unique program for natural sciences.

    Fujiwara, M, Aoki, S, Katayama, M, Sakayama, H, Shibao, H, Sekimoto, H, Nagata, Y, Fukui, A, Mizusawa, N, Michiue, T, Kato-Minoura, T

    The 48th Annual Meeting of the Japanese Society of Plant Physiologists  2007.3 

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  • Introductory course in life science experiments at the University of Tokyo: Development of experimental items and DVD materials.

    Mizusawa, N, Aoki, S, Katayama, M, Sakayama, H, Shibao, H, Sekimoto, H, Nagata, Y, Fukui, A, Fujiwara, M, Michiue, T, Kato-Minoura, T

    The 48th Annual Meeting of the Japanese Society of Plant Physiologists  2007.3 

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  • 東京大学における基礎生命科学実験:「ゆとり 教育第一世代」の大学新入生への開講に向けて

    柴尾晴信, 青木誠志郎, 片山光徳, 坂山英俊, 福井彰雅, 藤原誠, 水澤直樹, 道上達男, 箕浦高子

    日本動物行動学会第２５回大会  2006.10 

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  • Introductory course in laboratory experiments (Basic life sciences) at the University of Tokyo (1): Separation of photosynthesis-related proteins by SDS-PAGE.

    Mizusawa, N, Katayama, M, Sakayama, H, Shibao, H, Fukui, A, Fujiwara, M, Michiue, T, Kato-Minoura, T

    The 70th Annual Meeting of the Botanical Society of Japan  2006.9 

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  • Introductory course in laboratory experiments (Basic life sciences) at the University of Tokyo (2): Diversity of plant reproduction processes.

    Aoki, S, Katayama, M, Sakayama, H, Shibao, H, Fukui, A, Fujiwara, M, Mizusawa, N, Michiue, T, Kato-Minoura, T

    The 70th Annual Meeting of the Botanical Society of Japan  2006.9 

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  • Introductory Course in Laboratory Experiments (Basic Life Sciences) at the University of Tokyo: Developing a new curriculum.

    Kato-Minoura, T, Aoki, S, Katayama, M, Sakayama,H, Shibao, H, Nagata, Y, Fukui, A, Fujiwara, M, Mizusawa, N, Michiue, T

    The 77th Annual Meeting of the Zoological Society of Japan  2006.9 

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  • 私の仕事と後輩へのメッセージ

    箕浦高子

    名古屋大学女子学生エンカレッジセミナー  2006.1 

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  • 平成18年度からの基礎実験について（生物部会）

    箕浦高子

    東京大学教養学部附属教養教育開発機構公開シンポジウム「東京大学教養学部における基礎実験教育-平成18年度に向けた取り組み-」  2005.12 

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  • テトラヒメナ繊毛形成に関与する新奇アクチン関連タンパク質tArp

    Takako Kato-Minoura, Sayaka Kuribara, Mai Kato, Osamu Numata

    The 43rd Annual Meeting of the Biophysical Society of Japan  2005.11 

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  • A novel class XIX, myosins from Tetrahymena thermophila.

    Iwataki, Y, Gonda, K, Kato-Minoura, T, Numata, O

    The 76th Annual Meeting of the Zoological Society of Japan  2005.10 

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  • Identification of a novel actin-related protein in Tetrahymena cilia.

    Kato-Minoura, T, Kuribara, S, Kato, M, Numata, O

    The 76th Annual Meeting of the Zoological Society of Japan  2005.10 

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  • A novel class XIX, unconventional myosins from Tetrahymena thermophila

    Iwataki, Y, Someya, S, Gonda, K, Kato-Minoura, T, Numata, O

    The 57th Annual Meeting of the Japan Society for Cell Biology  2004.5 

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  • A novel family of unconventional actins in volvocalean algae

    Kato-Minoura, T, Okumura, M, Hirono, M, Kamiya, R

    11th International Conference on the Cell and Molecular Biology of Chlamydomonas  2004.5 

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  • テトラヒメナの新規ミオシンの探索

    岩滝仁範, 染矢晋太郎, 権田幸祐, 箕浦高子, 沼田治

    日本原生動物学会第３６回大会  2003.11 

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  • テトラヒメナ新規アクチン関連タンパク質（tArp）の繊毛形成における機能

    Takako Kato-Minoura

    The 74th Annual Meeting of the Zoological Society of Japan  2003.9 

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  • Distribution of sterol-rich membrane domains in Tetrahymena during cytokinesis.

    Kuribara, S, Kato-Minoura, T, Matsumira, T, Numata, O

    The 74th Annual Meeting of the Zoological Society of Japan  2003.9 

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  • Identification of novel actin-related proteins in Tetrahymena thermophila.

    Kato-Minoura, T, Kato, M, Kuribara, S, Numata, O

    The 56th Annual Meeting of the Japan Society for Cell Biology  2003.5 

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  • Identification of novel actin-related proteins and a small G protein in Tetrahymena thermophila.

    Kato-Minoura T, Kato M, Kuribara S, Numata, O

    The 73rd Annual Meeting of the Zoological Society of Japan  2002.9 

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  • Expression of novel unconventional actin upon deflagellation.

    Hirono, M, Uryu, S, Kato-Minoura, T, Kamiya, R

    The 73rd Annual Meeting of the Zoological Society of Japan  2002.9 

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  • A novel actin family in volvocalean.

    Kato-Minoura, T, Okumura, M, Hirono, M, Kamiya, R

    The 55th Annual Meeting of the Japan Society for Cell Biology  2002.5 

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  • A novel actin family in volvocalean

    Kato-Minoura, T, Okumura, M, Hirono, M, Kamiya, R

    The American Society for Cell Biology Forty-first Annual Meeting  2001.12 

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  • Effects of the two Chlamydomonas actin genes expressed with upstream regions exchanged.

    箕浦高子, 広野雅文, 神谷 律

    The 54th Annual Meeting of the Japan Society for Cell Biology  2001.5 

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  • クラミドモナスの非保守的アクチン:発現と鞭毛への局在

    広野雅文, 瓜生さと美, 尾崎 心, 箕浦高子, 神谷 律

    日本原生動物学会第３３回大会  2000.11 

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  • Expression and localization of a divergent actin in Chlamydomonas

    Hirono, M, Uryu, S, Ozaki, K, Kato-Minoura, T, Kamiya, R

    Ninth International Conference on the Cell and Molecular Biology of Chlamydomonas  2000.5 

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  • クラミドモナスの非保守的アクチン（NAP）の発現と細胞内局在．

    Masafumi Hirono, Satomi Uryu, Takako Kato-Minoura, Ritsu Kamiya

    The 52nd Annual Meeting of the Japan Society for Cell Biology  1999.8 

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  • Recovery of flagellar inner-arm dynein and the fertilization tubule in Chlamydomonas ida5 mutant by transformation with actin genes

    Hirono, M, Ohara, A, Kato-Minoura, T, Kamiya, R

    COE International Conference "Molecular Mechanism of Intracellular Transports: The Roles of Kinesin and Dynein Superfamily Protein"  1998.8 

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  • Increased expression of an actin-like protein in the Chlamydomonas mutant ida5 that lacks the conventional actin gene

    Kato-Minoura, T, Ohara, A, Uryu, S, Hirono, M, Kamiya, R

    Eighth International Conference on the Cell and Molecular Biology of Chlamydomonas  1998.6 

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  • Chlamydomonas flagellar inner-arm dynein mutant, ida5, expresses a highly diverged actin

    Kato-Minoura, T, Uryu, S, Hirono, M, Kamiya, R

    The American Society for Cell Biology Thirty-seventh Annual Meeting  1997.11 

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  • A novel actin in Chlamydomonas.

    Kato-Minoura, T. Uryu, S, Hirono, M, Kamiya, R

    The 50th Annual Meeting of the Japan Society for Cell Biology  1997.9 

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  • Rescue of a Chlamydomonas flagellar inner-arm dynein mutant, ida5, by the wild-type actin gene.

    Ohara, A, Kato-Minoura, T, Kamiya, R, Hirono, M

    The 50th Annual Meeting of the Japan Society for Cell Biology  1997.9 

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  • Absence of conventional actin in the Chlamydomonas inner-arm dynein mutant ida5.

    Kato-Minoura, Hirono, M, Kamiya, R

    The 49th Annual Meeting of the Japan Society for Cell Biology  1996.10 

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  • Chlamydomonas inner-arm dynein mutant, ida5, has a mutation in the unique actin-encoding gene

    Kato-Minoura, T, Hirono, M, Kamiya, R

    Seventh International Conference on the Cell and Molecular Biology of Chlamydomonas  1996.5 

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  • Chlamydomonas inner-arm dynein mutant, ida5, has a mutation in the actin-encoding gene.

    Kato, T, Hirono, M, Kamiya, R

    The 48th Annual Meeting of the Japan Society for Cell Biology  1995.10 

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  • Chlamydomonas inner-arm dynein mutant, ida5, has altered actin.

    Takako Kato, Yasuko Sugase, Masafumi Hirono, Ritsu Kamiya

    The 66th Annual Meeting of the Zoological Society of Japan  1995.9 

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  • A novel kinesin-like protein with a C-terminal motor domain in mammalian mitotic spindles is a minus-end directed motor

    Kuriyama, R, Kofron, M, Essner, R, Omoto, C. K, Dragas-Graonic, S, Kato, T, Khodjakov, A. L

    The American Society for Cell Biology Thirty-fourth Annual Meeting  1994.11 

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  • Isolation of two flagellar mutants, ida5 and ida6, that lack subsets of inner-arm dynein heavy chains

    Kato, T, Kagami, O, Yagi, T, Kamiya, R

    Sixth International Conference on the Cell and Molecular Biology of Chlamydomonas  1994.5 

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  • Novel Chlamydomonas mutants lacking subsets of inner-arm dynein heavy chains.

    Takako Kato, Osamu Kagami, Toshiki Yagi, Ritsu Kamiya

    The 64th Annual Meeting of the Zoological Society of Japan  1993.11 

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  • Identification of a novel kinesin-like protein with C-terminal motor domain from mitotic spindles

    Dragas-Graonic, S, Kato, T, Khodjakov, A. L, Kofron, M, Kuriyama, R

    The American Society for Cell Biology Thirty-third Annual Meeting  1993.10 

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  • クラミドモナスの新しいダイニン内腕欠失変異株

    Takako Kato, Osamu Kagami, Toshiki Yagi, Ritsu Kamiya

    The 31st Annual Meeting of the Biophysical Society of Japan  1993.9 

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  • Molecular cloning of the CHO2 antigen - an essential component of the centrosome for nucleation of microtubule in vitro

    Khodjakov, A. L, Kato, T, Kuriyama, R

    The American Society for Cell Biology Thirty-second Annual Meeting  1992.9 

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Awards

  • Olympus BioScape 2014 Competition Honorable Mentions

    2014.12   Olympus  

    Kentaro Kabuki, Noriko Ueki, Takako Kato-Minoura

  • Zoological Science Award

    2006.9   The Zoological Society of Japan   "Impaired flagellar regeneration due to uncoordinated expression of two divergent actin genes in Chlamydomonas. Zoological Science. 22 (5), pp. 571-577"

    Kato-Minoura,Takako

  • 平成15年度社団法人日本動物学会 女性研究者奨励ＯＭ賞

    2003.9   The Zoological Society of Japan   テトラヒメナ新奇アクチン関連タンパク質(tArp)の繊毛形成における機能

    Kato-Minoura, Takako

  • CSF Award

    1999.8   Japan Society for Cell Biology   "Recovery of flagellar inner-arm dynein and the fertilization tubule in Chlamydomonas ida5 mutant by transformation with actin genes. Cell Structure and Function. 23 (5), pp. 273-281"

    Ohara,A, Kato-Minoura,T, Kamiya,R, Hirono, M

Research Projects

  • クラミドモナスを用いた改変型細胞骨格のin vivo機能解析モデルの開発

    2018.4 - 2020.3

    2018年度 中央大学特定課題研究費 

    箕浦高子

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    Grant type：Competitive

    Grant amount： \2350000

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  • チューブリン遺伝子破壊株による微小管機能の順遺伝学・逆遺伝学的研究

    2017.4 - 2020.3

    2017年度理工学研究所共同研究第１類 

    箕浦高子

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    Grant type：Competitive

    Grant amount： \4676200

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  • Dynamic changes of tubulin dimer configuration on a scale of sub-second revealed by high-flux X-ray fiber diffraction analysis

    Grant number：16K07328  2016.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)  Chuo University

    Kamimura Shinji

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    Grant amount： \4940000 （ Direct Cost: \3800000 、 Indirect Cost： \1140000 ）

    Microtubules are assembled from tubulin dimers that are arranged in a semi-crystal lattice with high regularity. Dynamics of tubulin dimer within microtubules is thus expected to be directly affecting the stability of microtubules, however, it has been difficult to quantitatively describe such properties. By analyzing the time course of pattern changes in X-ray fiber diffractions from aligned microtubules with a high-flux synchrotron beam of BL40XU (SPring-8), we found microtubules showed rapid elongation in the axial tubulin repeat and the concomitant increase of structural flexibility with a time constant of about 0.2 s after applying paclitaxel, microtubule-stabilizer. Contrasting effects were found for laulimalide, another type of microtubules stabilizer.

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  • 細胞にアクチンは必要かークラミドモナスを用いた検証ー

    2014.4 - 2017.3

    文部科学省  科学研究費助成事業 挑戦的萌芽研究 

    箕浦 高子

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    Grant type：Competitive

    Grant amount： \3109249

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  • 微小管ドラッグ感受性突然変異株によるチューブリン機能ドメインの同定

    2013.4 - 2016.3

    2013年度理工学研究所共同研究第１類 

    箕浦高子

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount： \3480000

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  • 緑藻類に存在する非保守的アクチン（NAP）の進化的位置づけと機能の解明

    2011.4 - 2013.3

    2011年度 中央大学特定課題研究費 

    箕浦高子

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount： \1000000

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  • 緑藻クラミドモナスの新奇アクチン(NAP)の分子特性

    2010.4 - 2012.3

    2010年度理工学研究所共同研究第１類 

    箕浦高子

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount： \2500000

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  • 真核生物の鞭毛・繊毛形成の制御機構の解明（遺伝子発現と鞭毛内輸送IFTの連関について）

    2008.9 - 2009.9

    日揮・実吉奨学会研究助成金(民間助成金) 

    箕浦高子

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount： \1000000

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  • 収縮環におけるアクチン細胞骨格のダイナミクス

    2006.4 - 2009.3

    文部科学省  科学研究費補助金（日本学術振興会・文部科学省）－若手研究(B) 

    箕浦高子

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount： \3850000

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  • プロフィリンによる収縮環形成制御機構の解明

    2006.8 - 2007.7

    成茂動物科学振興基金 

    箕浦高子

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount： \500000

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  • Xenopus培養細胞へのsiRNA技術適用

    2004.4 - 2005.3

    東京大学21世紀COE「融合科学創成ステーション」奨励研究 

    箕浦高子

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount： \500000

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  • クラミドモナス新奇アクチン(NAP)の進化的位置づけと機能の解明

    Grant number：14780553  2002 　 　

    日本学術振興会  科学研究費助成事業  若手研究(B)  岡崎国立共同研究機構

    箕浦 高子

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    Grant amount： \1100000 （ Direct Cost: \1100000 ）

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  • クラミドモナス新奇アクチンの発現制御と細胞内機能の解明

    Grant number：12780544  2000 - 2001

    日本学術振興会  科学研究費助成事業  奨励研究(A)  岡崎国立共同研究機構

    箕浦 高子

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    Grant amount： \2400000 （ Direct Cost: \2400000 ）

    クラミドモナスには保守的配列のアクチンと、極めてdivergentな配列のNAPの2種のアクチン遺伝子が存在し、互いに異なる発現制御を受けている。両者の機能的差異と相関を理解することが重要と考えられるが、NAP独自の機能やその起源は謎である。今年度は昨年に引き続き、キメラ遺伝子による両者の人為的発現制御の効果を検討するとともに、オオヒゲマワリ目におけるNAPホモログを同定し、分子系統的解析を行った。
    1.キメラ遺伝子の導入によるNAP・アクチンの人為的発現制御
    翻訳開始点(ATG)を境に、NAPの上流領域(発現制御領域)とアクチンのタンパク質コード領域をつなげたキメラ遺伝子(pNAPΔpA7)、およびアクチンの上流領域とNAPのコード領域をつなげた遺伝子(pA7ΔpNAP)をそれぞれ作成し、アクチン欠損株に導入した。前者導入株では鞭毛形成能が低下した。一方、後者導入株では顕著な鞭毛形成活性を示した。また、前者導入株をさらにmutagenizeした株ではNAPの発現量が極端に低下し、鞭毛形成能も元株よりさらに低下した。以上から、NAPがタンパク量依存的に、あるいはup-regulationに伴って鞭毛形成に関与する可能性が示唆された。以上は日本細胞生物学会第54回大会にて発表した。
    2.オオヒゲマワリ目におけるアクチンファミリーの存在様式とNAPの起源
    C. moewusii,とV. carteriについて、NAPホモログを同定した。前者については未同定だったアクチンホモログも同定した。分子系統解析の結果、NAPと2種のNAPホモログがアクチンとは起源を異にする、新グループを形成することが判った。V. carterのNAPはC. reinhardtiiのNAPと高い相同性を示し、両種がきわめて近縁であるとする説を支持した。以上は米国細胞生物学会第41回年会にて発表した。

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  • フラミドモナスの新奇アクチン(NAP)の構造と機能

    Grant number：10780447  1998 - 1999

    日本学術振興会  科学研究費助成事業  奨励研究(A)  岡崎国立共同研究機構

    箕浦 高子

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    Grant amount： \1900000 （ Direct Cost: \1900000 ）

    クラミドモナスには骨格筋アクチンと90%のアミノ酸相同性を持つアクチンと、それとは非常に異なる配列を持つNAP(アクチンとの相同性64%)の、二種のアクチン遺伝子が存在し、互いに排他的に発現している。一生物種内に保守的なアクチンと非保守的なNAPが共存している例はこれまでに報告がない。今年度は2種のアクチンの機能的差異を検討するために、分裂酵母発現系を用いた機能解析を行った。また、他生物種におけるNAPホモログの有無を検討した。
    1.アクチンとNAPの酵母発現モデルの作成・解析
    NAP、及びアクチン遺伝子を分裂酵母の発現用ベクター(pREP1、pREP81)に導入した。NAP-pREP1で形質転換した分裂酵母株は細胞質分裂が著しく阻害され、多核の長い細胞が多く現れた。2割程度の細胞では抗NAP抗体による染色で分列環に似たリング状構造が確認され、リング内に少量の内在性アクチンが共局在した。
    2.他生物種におけるNAPの存在の有無
    近縁種のボルボックスを用い、NAP遺伝子ホモログの有無をサザンブロット法により検討した。NAPのcDNAコード領域の中間部をプローブに用い、ハイブリダイゼーションを行ったところ、反応するバンドは全く検出されなかった。徐々にストリンジェンシーを下げると、ボルボックスのアクチン遺伝子に相当するバンドが薄く検出された。ボルボックス・アクチンとNAPのアミノ酸配列相同性は65%である。実験結果はこの生物に少なくともアクチン以上にNAPと相同性を持つようなNAPホモログは存在しないことを強く示唆した。これは両種のアクチンが98%の高い相同性を持つこととは対照的である。

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Allotted class

  • 2024   Molecular Cell Biology   Department

  • 2024   Molecular Genetics   Department

  • 2024   Graduation Thesis Ⅰ   Department

  • 2024   Graduation Thesis Ⅱ   Department

  • 2024   Applied Biology   Department

  • 2024   Basic Biotechnology   Department

  • 2024   English for Bioscience - Advanced Course 1   Department

  • 2024   English for bioscience - advanced course 2   Department

  • 2024   English for bioscience - basic course   Department

  • 2024   Experimental Course of Molecular Genetics   Department

  • 2024   Doctoral Research Ⅰ   Graduate school

  • 2024   Doctoral Research Ⅱ   Graduate school

  • 2024   Doctoral Research Ⅲ   Graduate school

  • 2024   Doctoral Research Ⅳ   Graduate school

  • 2024   Doctoral Research Ⅴ   Graduate school

  • 2024   Doctoral Research Ⅵ   Graduate school

  • 2024   Special lecture on Biological Sciences   Graduate school

  • 2024   Master's Research Ⅰ   Graduate school

  • 2024   Master's Research Ⅲ   Graduate school

  • 2024   Master's Research Ⅱ   Graduate school

  • 2024   Master's Research Ⅳ   Graduate school

  • 2024   Regulation of Cell Function   Graduate school

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